Bunyavirus Protein Transport and Assembly
One of the distinctive features of the Bunyaviridae is their intracellular maturation process, which occurs by budding at smooth-surfaced membranes in the Golgi region (Murphy et al. 1973). Virions bud into the Golgi cisternae and are then transported and released at the cell surface probably by a vesicular transport process utilizing the exocytic pathway. Before assembly of the virus particles, all structural components including proteins as well as viral genome segments must reach the site where virus particles are formed. Recent studies of the envelope glycoproteins have emphasized their role in determining the site of virus maturation. Bunyavirus glycoproteins accumulate at the membranes of the Golgi apparatus prior to virus assembly, indicating that the glycoproteins may serve to direct other structural components to the site of maturation. Recently it has become evident that bunyavirus glycoproteins expressed from cloned cDNAs are retained in the Golgi complex and thus behave similarly to resident proteins of the Golgi complex.
KeywordsGolgi Complex Vesicular Stomatitis Virus Rift Valley Fever Semliki Forest Virus Golgi Membrane
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