Bunyavirus Protein Transport and Assembly
One of the distinctive features of the Bunyaviridae is their intracellular maturation process, which occurs by budding at smooth-surfaced membranes in the Golgi region (Murphy et al. 1973). Virions bud into the Golgi cisternae and are then transported and released at the cell surface probably by a vesicular transport process utilizing the exocytic pathway. Before assembly of the virus particles, all structural components including proteins as well as viral genome segments must reach the site where virus particles are formed. Recent studies of the envelope glycoproteins have emphasized their role in determining the site of virus maturation. Bunyavirus glycoproteins accumulate at the membranes of the Golgi apparatus prior to virus assembly, indicating that the glycoproteins may serve to direct other structural components to the site of maturation. Recently it has become evident that bunyavirus glycoproteins expressed from cloned cDNAs are retained in the Golgi complex and thus behave similarly to resident proteins of the Golgi complex.
KeywordsCodon Fractionation Polypeptide Oligosaccharide Sorting
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- Alonso-Caplen FV, Matsuoka Y, Compans RW (1984) Transport of viral glycoproteins and its modulation by monensin. In: Compans RW, Bishop DHL (eds) Segmented negative strand viruses. Academic, New York, pp 179–188Google Scholar
- Hashimoto K, Erdei S, Karanen S, Saraste J, Kaarianen L (1981) Evidence for a separate signal sequence for the carboxy-terminal envelope glycoprotein E1 of Semliki Forest virus. J Virol 38: 24–40Google Scholar
- Lenard J, Compans RW (1974) The membrane structure of lipid-containing viruses. Biochim Biophys Acta 334: 51–94Google Scholar
- Parker MD, Smith JF, Dalrymple JM (1984) Rift Valley fever virus intracellular RNA: a functional analysis. In: Compans RW, Bishop DHL (eds) Segmented negative strand viruses. Academic, Orlando, pp 21–28Google Scholar
- Pensiero MN, Jennings GB, Schmaljohn CS, Hay J (1988) Expression of the Hantaan virus M genome segment by using a vaccinia virus recombinant. J Virus 62: 696–702Google Scholar
- Persson R, Wikstrom L, Pettersson RF (1989) Dimerization and intracellular transport of two Golgi-specific bunyavirus membrane glycoproteins. In: Compans RW, Helenius A, Oldstone MBA (eds) UCLA symposium on cell biology of virus entry, replication and pathogenesis. Liss, New York, pp 229–242Google Scholar
- Pugsley AP (1989) Protein targeting. Academic, San Diego Rice CM, Strauss JH (1981) Nucleotide sequence of the 265 mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins. Proc Natl Acad Sci USA 78: 2062–2066Google Scholar
- Schmaljohn CS, Patterson JL (1990) Bunyaviridae and their replication. In: Fields BN et al. (eds) Virology. Raven, New York, pp 1175–1194Google Scholar
- Wikstrom L, Persson R, Pettersson RF (1989) Intracellular transport of the G1 and G2 membrane glycoproteins of Uukuniemi virus. In: Kolakofsky D, Many B (eds) Genetics and pathogenicity of negative strand virus. Elsevier, New York, pp 33–41Google Scholar