Relationship of Cytochrome caa3 from Thermus thermophilus to Other Heme- and Copper-Containing Terminal Oxidases
Cytochrome oxidases are a key component of the energy metabolism of most aerobic organisms from mammals to bacteria. They are the final enzyme of the membrane-associated respiratory chain responsible for converting the chemical energy of reduced substrates to a transmembrane electrochemical potential, which is used by the cell for a wide variety of energy-requiring processes. The most widely studied oxidase is the cytochrome c oxidase (cytochrome aa3 oxidase) of the mammalian mitochondrion. This complex, integral membrane protein contains 13 subunits and 4 canonical metal centers : heme centers, a and a3 ; copper centers, CuA and CuB. It is responsible for electron transfer from reduced cytochrome c to dioxygen with the concomitant reduction of dioxygen to water and the coupled vectorial transfer of protons across the mitochondrial membrane (see Chan and Li 1990; Palmer 1987 for recent reviews).
KeywordsCysteine Carbon Monoxide Bacillus Polypeptide Methionine
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