Abstract
A rapid diagnostic test for the detection of Listeria monocytogenes (Lm) using the polymerase chain reaction (PCR) was developed using primers flanking a sequence in the structural gene for the thiol-activated haemolysin, listeriolysin O.The sequence detected was specific for Lm and was not found in other haemolytic or non-haemolytic Listeria species, or in species of other genera known to produce thiol-activated cytolysins. This technique detected Lm in acute-phase sera from patients with Lm bacteraemia but not in samples of sera from patients with bacteraemia associated with the isolation of other bacterial species.
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© 1991 Springer-Verlag Berlin Heidelberg
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Kite, P., Kerr, K.G., Taylor, G., Millar, M.R., Michael, R. (1991). Rapid Diagnosis of Human Listeriosis Using the Polymerase Chain Reaction. In: Rolfs, A., Schumacher, H.C., Marx, P. (eds) PCR Topics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75924-6_38
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DOI: https://doi.org/10.1007/978-3-642-75924-6_38
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-52934-7
Online ISBN: 978-3-642-75924-6
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