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Detection of Chlamydia Trachomatis by PCR

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PCR Topics

Abstract

Chlamydia trachomatis, an obligate intracellular parasite, is a common agent of sexually transmitted diseases, and the causative agent of several human clinical conditions including urethritis, cervititis, salpingitis, female infertility, lymphogranuloma venereum and trachoma. The diagnosis of C. trachomatis as a pathogen is currently difficult and expensive, because laboratory diagnosis is achieved by isolation in cell culture, a technique which is both cumbersome and time-consuming.

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References

  1. Dutilh B, Bebear C, Rodriguez P, Vekris A, Bonnet J, Garret M (1989). Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction. Res Microbiol 140: 7–16

    Article  PubMed  CAS  Google Scholar 

  2. Comanducci M, Ricci S, Ratti G (1988). The structure of a plasmid of Chlamydia trachomatis believed to be required for growth within mammalian cells. Mol Microbiol 2: 531–538

    Article  PubMed  CAS  Google Scholar 

  3. Griffais R, Thibon M (1989). Detection of Chlamydia trachomatis by the polymerase chain reaction. Res microbiol 140: 139–141

    Article  PubMed  CAS  Google Scholar 

  4. Hatt C, Ward ME, Clarke IN (1988). Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1 evidence for involvement in DNA replication. NUCL Acids Res 16: 4053–4067

    Article  PubMed  CAS  Google Scholar 

  5. Palmer L, Falfow S (1986). A common plasmid of the genus Chlamydia trachomatis. Plasmid 16: 52–62

    Article  PubMed  CAS  Google Scholar 

  6. Pollard DR, Tyler SD, Ngo CW, Rozee KR (1989). A polymerase chain reaction (PCR) protocol for the specific detection of Chlamydia spp. Mol Cell Probes 3: 383–389

    Article  PubMed  CAS  Google Scholar 

  7. Ripa KV, Mardh PA (1977). Cultivation of Chlamydia trachomatis in cyclohexamidetreated Mc Coy cells. J Clin Microbiol 6: 329–331

    Google Scholar 

  8. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Ehrlich HA (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491

    Article  PubMed  CAS  Google Scholar 

  9. Schachter J, Martin DH (1987). Failure of multiples passages to increase chlamydial recovery. J Clin Microbiol 25: 1851–1853

    PubMed  CAS  Google Scholar 

  10. Sriprakash KS, Mac Avoy ES (1987). Characterisation and sequence of a plasmid from the trachoma biovar of Chlamydia trachomatis. Plasmid 18: 205–214

    Article  PubMed  CAS  Google Scholar 

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© 1991 Springer-Verlag Berlin Heidelberg

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Petit, M.C., Francois, M.H., Berriche, S., Burckel, A., Lucotte, G. (1991). Detection of Chlamydia Trachomatis by PCR . In: Rolfs, A., Schumacher, H.C., Marx, P. (eds) PCR Topics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75924-6_36

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  • DOI: https://doi.org/10.1007/978-3-642-75924-6_36

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-52934-7

  • Online ISBN: 978-3-642-75924-6

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