Hepatitis B Virus DNA — Identification by Polymerase Chain Reaction (PCR) and Correlation with Serological HBV Markers
The demonstration of hepatitis B virus DNA (HBV DNA) in blood is considered as an indication of virus replication and of a patient’s infectivity , The sensitivity of standard hybridization methods (e.g. dot-blot) which is in the range of 30.000 HBV viruses is not sufficient to recognize a minimal virus replication and can therefore not distinguish infectious from non infectious patients. The application of PCR techniques may improve the detection of HBV DNA. The purpose of the present investigation is to compare the results of the PCR method with conventional dot-blot hybridization in 418 blood samples of patients with HBV infections and of healthy persons.
KeywordsMigration HPLC Hepatitis DMSO Agarose
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