Advertisement

PCR Topics pp 136-141 | Cite as

Detection of Epstein-Barr Virus Genomes in Lymphoproliferative Diseases

  • Michael Hummel
  • Hermann Herbst
  • Gerald Niedobitek
  • Michael Kneba
  • Teresa Finn
  • Ioannis Anagnostopoulos
  • Michael Bergholz
  • Gerhard Krieger
  • Harald Stein

Abstract

Epstein-Barr virus (EBV), the etiologic agent of infectious mononucleosis, has a clearly established association with endemic Burkitt’s lymphoma (BL), nasopharyngeal carcinoma (NPC), and secondary B-cell proliferations in immunosuppressed individuals [1,2]. Epidemiologic studies have linked Hodgkin’s disease (HD), which accounts for 30–50% of malignant lymphomas in Western countries, to previous infectious mononucleosis [3]. Increased EBV-specific antibody titers were frequently observed in HD patients [4,6]. Recently, DNA filter hybridization studies pointed to an association of EBV with up to 20% of isolated cases of HD [7,9]. In EBV-positive HD cases studied in more detail, the viral DNA was monoclonal in origin and confined to Hodgkin and Reed-Sternberg (H and RS) cells that constitute the malignant cell population of HD [10,11]. However, since H and RS cells often account for less than 1% of the total cell number in HD-afflicted lymph nodes, it is possible that EBV escaped detection by filter hybridization methods in many HD cases. Therefore, we screened 198 HD cases, 151 non-Hodgkin lymphomas (NHL), and 34 non-malignant lymph node biopsies, all of which were clinically unrelated to HIV-infection, with the highly sensitive polymerase chain reaction (PCR) [12].

Keywords

Infectious Mononucleosis Chronic Lymphatic Leukemia Sensitive Polymerase Chain Reaction Specific Polymerase Chain Reaction Product Malignant Cell Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Zur Hausen H, Schulte-Holthausen H, Klein G, Henle W, Henle G, Clifford P, Santesson L: Nature 1970, 228:1056–1058PubMedCrossRefGoogle Scholar
  2. 2.
    Cleary ML, Warnke R, Sklar J: Lancet 1984, 2:489–493PubMedCrossRefGoogle Scholar
  3. 3.
    Gutensohn NM, Cole P: Sem Oncol 1980, 7:92–102Google Scholar
  4. 4.
    Johansson B, Klein G, Henle W, Henle G: Int J Cancer 1970, 6:450–462PubMedCrossRefGoogle Scholar
  5. 5.
    Hesse J, Andersen E, Levine HP, Ebbesen P, Halberg P, Reisher JI: Int J Cancer 1973, 11:237–343PubMedCrossRefGoogle Scholar
  6. 6.
    Mueller N, Evans A, Harris NL, Comstock GW, Jellum E, Magnus K, Orentreich N, Polk F, Vogelman J: N Engl J Med 1989, 320:689–695PubMedCrossRefGoogle Scholar
  7. 7.
    Weiss LM, Strickler JG, Warnke RA, Purtilo DT, Sklar J: Am J Pathol 1987, 129:86–91PubMedGoogle Scholar
  8. 8.
    Staal SP, Ambinder R, Beschorner WE, Hayward G, Mann R: Am J Clin Pathol 1989, 91:1–5PubMedGoogle Scholar
  9. 9.
    Herbst H, Tippelmann G, Anagnostopoulos, Gerdes J, Schwarting R, Boehm T, Pileri S, Jones DB, Stein H: Leuk Res 1989, 13:103–116PubMedCrossRefGoogle Scholar
  10. 10.
    Weiss LM, Movahed AM, Warnke RA, Sklar J: N Engl J Med 1989, 320:502–506PubMedCrossRefGoogle Scholar
  11. 11.
    Anagnostopoulos I, Herbst H, Niedobitek G, Stein H: Blood 1989, 74:810–816PubMedGoogle Scholar
  12. 12.
    Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA: Science 1988, 239:487–491PubMedCrossRefGoogle Scholar
  13. 13.
    Polack A, Hartl G, Zimber U, Freese UK, Laux G, Takaki K, Hohn B, Gissmann L, Bornkamm GW: Gene 1984, 27:279–288PubMedCrossRefGoogle Scholar
  14. 14.
    Cheung A, Kieff E: J Virol 1982, 44:286–291PubMedGoogle Scholar
  15. 15.
    Niedobitek G, Hamilton-Dutoit S, Herbst H, Finn T, Vetner M, Pallesen G, Stein H: Hum Pathol 1989, 20:796–799PubMedCrossRefGoogle Scholar
  16. 16.
    Weiss LM, Movahed LA: Am J Pathol 1989, 134:651–659PubMedGoogle Scholar
  17. 17.
    Hamilton-Dutoit SJ, Pallesen G, Karkov J, Skinhoj P, Franzmann MV, Pedersen C: Lancet 1989, 1:554–555PubMedCrossRefGoogle Scholar
  18. 18.
    Katz BZ, Raab-Traub N, Miller G: J Infect Dis 1989, 160:589–598PubMedCrossRefGoogle Scholar
  19. 19.
    Epstein MA, Morgan AJ: In: Epstein MA, Achong BG (eds), The Epstein-Barr Virus: Recent Advances. Heinemann, London, 1986, pp 271–289Google Scholar
  20. 20.
    Morgan AJ, Finerty S, Lovgren K, Scullion FT, Morein B: J gen Virol 1988, 69:2093–2096PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Michael Hummel
  • Hermann Herbst
  • Gerald Niedobitek
  • Michael Kneba
  • Teresa Finn
  • Ioannis Anagnostopoulos
  • Michael Bergholz
  • Gerhard Krieger
  • Harald Stein

There are no affiliations available

Personalised recommendations