Sequencing of PCR Products — Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes
The polymerase chain reaction facilitates DNA sequence analysis due to the simplicity and high sensitivity of the procedure. In many cases the detection of a previously characterized sequence, restriction site or sequence polymorphism is the only information required. This information is most conveniently obtained either by determination of the size of the amplified product on an agarose gel, analysis after restriction enzyme digestion or hybridization using allele-specific oligonucleotides as probes.
KeywordsGene Conversion Carrier Testing Light Chain Gene Splice Site Junction Light Chain Locus
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- 9.Koeberl, D. D., Bottema, C. D. K., Ketterling, R. P., Bridges, P. J., Lillicrap, D. P., and Sommer, S. S. (1990). Mutations causing hemophilia B: direct estimate of the underlying rates of spontaneous germline transitions, trans versions, and deletions in a human gene. Am. J. Hum. Genet., (in press).Google Scholar
- 10.Bottema, C. D., Koeberl, D. D., and Sommer, S. S. (1989). Direct carrier testing in 14 Families with Hemophilia B. Lancet, 526–529.Google Scholar