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Sequencing of PCR Products — Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes

  • Jean-Marie Buerstedde
  • Steve S. Sommer

Abstract

The polymerase chain reaction facilitates DNA sequence analysis due to the simplicity and high sensitivity of the procedure. In many cases the detection of a previously characterized sequence, restriction site or sequence polymorphism is the only information required. This information is most conveniently obtained either by determination of the size of the amplified product on an agarose gel, analysis after restriction enzyme digestion or hybridization using allele-specific oligonucleotides as probes.

Keywords

Gene Conversion Carrier Testing Light Chain Gene Splice Site Junction Light Chain Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Jean-Marie Buerstedde
  • Steve S. Sommer

There are no affiliations available

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