PCR Topics pp 66-68 | Cite as

False Positive Reactions in PCR

  • Erik C. Böttger

Abstract

The polymerase chain reaction (PCR) is a recently developed powerful method with many applications in molecular biology and medical diagnostics. The exquisite sensitivity of PCR is its power, but also its weakness. Even the smallest amount of contaminating DNA can be amplified, resulting in misleading or ambiguous results. Accordingly, it is necessary to take strict precautions to avoid DNA contamination when using PCR [1,2].

Keywords

Agarose Bromide Ethidium 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Erik C. Böttger

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