Abstract
PCR primers covalently labeled with biotin and a fluorescent dye allow immobilization and separation of the products which, subsequently, can be quantitatively analysed. The procedure we have developed circumvents electrophoretic separation and radioactive labeling. Exact quantitative analysis of reaction products is feasible during the logarithmic phase of amplification and with internal standardization. PCR is carried out in a newly developed thermocycler with exceptional heating and cooling rates. The thermocycler performs 30 cycles in 2 hours.
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© 1991 Springer-Verlag Berlin Heidelberg
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Landgraf, A., Pingoud, A., Reckmann, B. (1991). Quantitative Analysis of Polymerase Chain Reaction (PCR) Products by Means of Fluorescence Detection. In: Rolfs, A., Schumacher, H.C., Marx, P. (eds) PCR Topics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75924-6_11
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DOI: https://doi.org/10.1007/978-3-642-75924-6_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-52934-7
Online ISBN: 978-3-642-75924-6
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