Analysis of Epstein-Barr Virus Gene Expression in Lymphomas Derived from Normal Human B Cells Grafted into SCID Mice
The recent demonstration that C.B-17scid/scid mice (hereafter referred to as SCID mice) could be grafted successfully with human haematopoietic cells (Hosier et al., 1988; McCune et al., 1988; Kamel-Reid and Dick, 1988) has opened the way to the use of the SCID-hu mouse as a model for studying human immune-function in vivo. However, if SCID-hu mice are generated by intraperitoneal injection of about 5×107 human peripheral blood lymphocytes (PBLs), then consideration must be given to the fact that human B cell lymphomas regularly develop between 2–4 months post-engraftment. The causal role of Epstein-Barr virus (EBV) in the development of these lymphomas has been demonstrated (Mosier et al., 1988). Thus, all of the lymphomas contained EBV DNA and, furthermore, lymphomas did not develop in mice engrafted with PBLs from donors where there was no serological evidence of EBV infection. In this preliminary report we have further characterised these EBV-positive lymphomas in order to determine whether they might be used as a model for EBV-associated malignancies.
KeywordsSCID Mouse Latent Membrane Protein Viral Capsid Antigen Virus Latent Gene Expression Human Haematopoietic Cell
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