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Analysis of Epstein-Barr Virus Gene Expression in Lymphomas Derived from Normal Human B Cells Grafted into SCID Mice

  • M. Rowe
  • L. S. Young
  • A. B. Rickinson
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 166)

Abstract

The recent demonstration that C.B-17scid/scid mice (hereafter referred to as SCID mice) could be grafted successfully with human haematopoietic cells (Hosier et al., 1988; McCune et al., 1988; Kamel-Reid and Dick, 1988) has opened the way to the use of the SCID-hu mouse as a model for studying human immune-function in vivo. However, if SCID-hu mice are generated by intraperitoneal injection of about 5×107 human peripheral blood lymphocytes (PBLs), then consideration must be given to the fact that human B cell lymphomas regularly develop between 2–4 months post-engraftment. The causal role of Epstein-Barr virus (EBV) in the development of these lymphomas has been demonstrated (Mosier et al., 1988). Thus, all of the lymphomas contained EBV DNA and, furthermore, lymphomas did not develop in mice engrafted with PBLs from donors where there was no serological evidence of EBV infection. In this preliminary report we have further characterised these EBV-positive lymphomas in order to determine whether they might be used as a model for EBV-associated malignancies.

Keywords

SCID Mouse Latent Membrane Protein Viral Capsid Antigen Virus Latent Gene Expression Human Haematopoietic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • M. Rowe
    • 1
  • L. S. Young
    • 1
  • A. B. Rickinson
    • 1
  1. 1.Department of Cancer Studies, Cancer Research Campaign Laboratories, School of Medical SciencesUniversity of BirminghamBirminghamUK

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