Recombination of the c-myc Gene with IgH Enhancer-Sμ Sequences in a Murine Plasmacytoma (DCPC 21) Without Visible Chromosomal Translocations

  • S. Ohno
  • S. Migita
  • S. Murakami
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 166)

Abstract

Murine plasmacytomas (MPCs) consistently exhibit chromosomal translocations involving chromosome 15 (t(12;15) or t(6;15)) (Ohno et al. 1979, 1984, 1988). These translocations of chromosomes juxtapose the proto-oncogene c-myc to the one of Ig gene loci (Cory 1986) . The juxtaposition of c-myc and the Ig gene strongly suggests that they play a critical genomic role in the development of MPCs probably by deregulating the c-myc gene expression.

Keywords

Sucrose Lymphoma Leukemia Recombination Agarose 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Committee on Standardized Genetic Nomenclature for Mice (1972) Standard karyotype of the mouse, Mus musculus. J Hered 63: 69–72Google Scholar
  2. Corcoran LM, Cory S, Adams J (1985) Transposition of the immunoglobulin heavy chain enhancer to the myc oncogene in a murine plasmacytoma. Cell 40: 71–79PubMedCrossRefGoogle Scholar
  3. Cory S (1986) Activation of cellular oncogene in hemopoietic cells by chromosome translocation. Adv Cancer Res 47: 189–234PubMedCrossRefGoogle Scholar
  4. Dreazen O, Rassool F, Sparkes RS, Klisak I, Goldman JM, Gale RP (1987) Do oncogenes determine clinical features in chronic myeloid leukemia? Lancet 1: 1402–1405PubMedCrossRefGoogle Scholar
  5. Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13PubMedCrossRefGoogle Scholar
  6. Gillies SD, Morrison SL, Oi VT, Tonegawa S (1983) A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. Cell 33: 717–728PubMedCrossRefGoogle Scholar
  7. Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83: 431–439PubMedCrossRefGoogle Scholar
  8. Kakkis E, Riggs KJ, Gillespie W, Calame K (1989) A transcriptional repressor of c-myc. Nature 339: 718–721PubMedCrossRefGoogle Scholar
  9. Klein G (1983) Specific chromosomal translocations and the genesis of B-cell-derived tumors in mice and men. Cell 32: 311–315PubMedCrossRefGoogle Scholar
  10. Marcu KB, Lang RB, Stanton LW, Harris LJ (1982) A model for the molecular requirements of immunoglobulin heavy chain class switching. Nature 298: 87–89PubMedCrossRefGoogle Scholar
  11. Merwin RM, Redmon LW (1963) Induction of plasma cell tumors and sarcoma in mice by diffusion chambers placed in the peritoneal cavity. J Natl Cancer Inst 31: 998–1007Google Scholar
  12. Morris CM, Reeve AE, Fitzgerald PH, Hollings PE, Beard MEJ, Heaton DC (1986) Genomic diversity correlates with clinical variation in Ph’-negative chronic myeloid leukemia. Nature 320: 281–283PubMedCrossRefGoogle Scholar
  13. Nesbitt MN, Francke U (1973) A system of nomenclature for band patterns of mouse chromosomes. Chromosoma 41: 145–158PubMedCrossRefGoogle Scholar
  14. Nikaido T, Nakai S, Honjo T (1981) Switch region of immunoglobulin Cμ gene is composed of single tandem repetitive sequences. Nature 292: 845–848PubMedCrossRefGoogle Scholar
  15. Ohno S, Babonits M, Wiener F, Spira J, Klein G, Potter M (1979) Nonrandom chromosome changes involving the Ig gene-carrying chromosomes 12 and 6 in pristane-induced mouse plasmacytomas. Cell 18 1001–1007PubMedCrossRefGoogle Scholar
  16. Ohno S, Migita S, Wiener F, Babonits M, Klein G, Mushinski, JF, Potter (1984) Chromosomal translocations activating myc sequences and transduction of v-abl are critical events in the rapid induction of plasmacytomas by pristane and Abelson virus. J Exp Med 159: 1762–1777PubMedCrossRefGoogle Scholar
  17. Ohno S, Takahashi R, Mihara K, Chen H-L, Migita S, Sugiyama T (1988) An atypical t(12;15) translocation of chromosomes found in murine plasmacytomas and activation of the c-myc oncogene. Int J Cancer 41: 595–600PubMedCrossRefGoogle Scholar
  18. Ohno S, Migita S, Murakami S (1989) C-myc-Sμ rearrangement in a murine plasmacytoma without visible chromosomal translocations. Oncogene 4: 1513–1517PubMedGoogle Scholar
  19. Rosenberg SM (1985) EcoK restriction during in vitro packaging of coliphage lambda DNA. Gene 39: 313–315PubMedCrossRefGoogle Scholar
  20. Sakano H, Maki R, Kurosawa Y, Roeder W, Tonegawa S (1980) Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature 286: 676–683PubMedCrossRefGoogle Scholar
  21. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467PubMedCrossRefGoogle Scholar
  22. Stanton LW, Yang J-Q, Eckhard LA, Harris LJ, Birshtein BK, Marcu KB (1984) Products of a reciprocal chromosome translocation involving tl c-myc gene in a murine plasmacytoma. Proc Natl Acad Sci USA 81: 829–833PubMedCrossRefGoogle Scholar
  23. Taub R, Kirsch I, Morton C, Lenoir G, Swan D, Tronick S, Aaronson S, Leder P (1982) Translocation of the c-myc gene into the immunoglobul: heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc Natl Acadc Sci USA 79: 7837–7841CrossRefGoogle Scholar
  24. Wang HC, Fedoroff S (1972) Banding in human chromosomes treated with trypsin. Nature (New Biol.) 235: 52–54CrossRefGoogle Scholar
  25. Zabarovsky ER, Allikmets RL (1986) An improved technique for the efficient construction of gene libraries by partial filling-in of cohesive ends. Gene 42: 119–123PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • S. Ohno
    • 1
  • S. Migita
    • 1
  • S. Murakami
    • 2
  1. 1.Department of Molecular Immunology, Cancer Research InstituteKanazawa UniversityKanazawaJapan
  2. 2.Division of Biophysics, Cancer Research InstituteKanazawa UniversityKanazawaJapan

Personalised recommendations