Abstract
We and others have speculated that elevated expression of c-myc plays an important role in the establishment of the transformed follicle, a preneoplastic lesion essential for the development of the ALV-induced bursal-dependent lymphoma (Baba and Humphries 1985, Clurman and Hayward 1989, Neiman et al 1985). The discovery of base substitutions and missense mutations in some tumor c- myc alleles raises the possibility that qualitative as well as quantitative alterations within the chicken c-myc proto-oncogene may play a role in the genesis and/or progression of bursal lymphomas (Westaway et al 1984). In order to assess the contribution that deregulated expression of an unaltered c-myc allele makes during ALV lymphomagenesis (Hayward et al 1981) , we have constructed a recombinant avian retrovirus, SNVc-myc, that encodes the second and third exons of normal chicken c-myc and expresses only the smaller of the two c-myc proto-oncogene products, pp62c-myc and pp59c-myc (Hann et al 1988). Ultimately, this virus will enable the effects of specific mutations within the context of wild-type pp59c-myc to be assessed.
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References
Adams JM, Harris AV, Pinkert CA, Corcoran LM, Alexander WS, Cory S, Palmieri RD and Brinster RL (1985) The c-myc oncogene driven by immunoglobulin enhancers induced lymphoid malignancy in transgenic mice. Nature 318:533–538
Baba TW and Humphries EH (1985) Formation of a transformed follicle is necessary but not sufficient for development of an avian leukosis virus-induced lymphoma. Proc Natl Acad Sci USA 82:213–216
Baumbach WR, Keath EJ and Cole MD (1986) A mouse c-myc retrovirus transforms established fibroblast lines in vitro and induces monocyte-macrophage tumors in vivo. J Virol 59:276–283
Bister K, Hayman MJ and Vogt PK (1977) Defectiveness of avian myelocytomatosis virus, MC29: Isolation of long term nonproducer cultures and analysis of virus specific polypeptide synthesis. Virology 82:431–448
Chen ISY, Mak TK, O’Rear JJ and Temin HM (1981) Characterization of reticuloendotheliosis virus Strain T DNA and isolation of a novel variant of reticuloendotheliosis virus Strain T by molecular cloning. J Virol 40:800–811
Clurman BE and Hayward WS (1989) Multiple proto-oncogene activations in avian leukosis virus-induced lymphomas: Evidence for stage-specific events. Mol Cell Biol 9:2657–2664
Dotto GP, Parada LF and Weinberg RA (1985) Specific growth response of ras-transformed embryo fibroblasts to tumor promoters. Nature 318:472–475.
Hann SR, King MW, Bentley DL, Anderson CW and Eisenmann RN (1988) A non-AUG translational initiation in c-myc exon 1 generates a N-terminally distinct protein whose synthesis is disrupted in Burkitt’s Lymphomas. Cell 52:185–195
Hayward WS, Neel BG, and Astrin SM (1981) Activation of a cellular one gene by promoter insertion. Nature 290:475–480
La Rocca SA, Grossi M, Falcone G, Alema S and Tato F (1989) Interaction with normal cells suppresses the transformed phenotype of v-myc-transformed quail muscle cells. Cell 58:123–131
Martin P, Henry C, Ferre F, Duterque-Coquillaud M, Lagrou C, Ghysdael J, Debuire B, Stehelin D and Saule S (1986) Transformation of quail embryo fibroblasts by a retrovirus carrying a normal human c-myc gene. Eur Mol Biol Org Jrnl 5:1529–1533
Neiman PE, Wolf C, Enrietto PJ and Cooper GM (1985) A retroviral myc gene induces preneoplastic transformation of lymphocytes in a bursal transplantation assay. Proc Natl Acad Sci USA 82:222–226
Ramsay G, Enrietto PJ, Graf T and Hayman MJ (1982) Recovery of myc-specific sequences by a partially transformation defective mutant of avian myelocytomatosis virus, MC29, correlates with the restoration of transforming activity. Proc Natl Acad Sci USA 79:6885–6889
Stoker MGP, Shearer M and O’Neill C (1966) Growth inhibition of polyoma transformed cells by contact with static normal fibroblasts. J Cell Sci 1:297–310
Vennstrom B, Sheiness D, Zabielski J and Bishop JM (1982) Isolation and characterization of c-myc, a cellular homologue of the oncogene (v-myc) of avian myelocytomatosis virus Strain 29. J Virol 42:773–779
Watanabe S and Temin HM (1983) Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Mol Cell Biol 3:2241–2249
Watson DK, Premkumar Reddy E, Duesberg PH and Papas TS (1983) Nucleotide sequence analysis of the chicken c-myc gene reveals homologous and unique coding regions by comparison with the transforming gene of avian myelocytomatosis virus MC29, Δgag-myc*. Proc Natl Acad Sci USA 80:2146–2150
Weiss RA (1970) The influence of normal cells on the proliferation of tumor cells in culture. Exp Cell Res 63:1–18
Westaway D, Payne G and Varmus HE (1984) Proviral deletions and oncogene base-substitutions in insertionally mutagenized c-myc alleles may contribute to the progression of avian bursal tumors. Proc Natl Acad Sci USA 81:843–847
Zhou R-P and Duesberg PH (1988) Myc proto-oncogene linked to retroviral promoter but not to enhancer, transforms embryo cells. Proc Natl Acad Sci USA 85:2924–2928
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© 1990 Springer-Verlag Berlin Heidelberg
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Humphries, E.H., Filardo, E.J. (1990). The Transforming Activity of PP59C-MYCis Weaker Than That of v-myc . In: Potter, M., Melchers, F. (eds) Mechanisms in B-Cell Neoplasia 1990. Current Topics in Microbiology and Immunology, vol 166. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75889-8_32
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DOI: https://doi.org/10.1007/978-3-642-75889-8_32
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