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Immortalization of Primary Murine B Lymphocytes with Oncogene-Containing Retroviral Vectors

  • R. W. Overell
  • K. E. Weisser
  • B. Hess
  • R. Goodwin
  • L. Clark
  • K. H. Grabstein
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 166)

Abstract

Various oncogenes have been implicated in B cell neoplasia, and the use of transgenic mouse models and in vivo experiments with oncogene-containing retroviral vectors has started to assign cooperative functions to these oncogenes in B cell transformation (Adams et al.,1985; Potter et al.,1987). In addition, much has been learned through the in vitro transformation of pre-B cells by retrovirally transferred oncogenes (Schwartz et al.,1986). However, the ability of oncogenes to immortalize primary mature (slg+) B cells has not been systematically explored. Indeed, it has been proposed that mature lymphocytes are refractory to infection with retroviruses. In these studies we have shown that mature B cells purified from murine spleen are readily infectable with ecotropic retroviruses, and have assayed a panel of oncogene-containing constructs for their ability to immortalize these primary cells in vitro.

Keywords

Murine Spleen Cell Surface Phenotype Drug Resistance Marker Immunoglobulin Enhancer Lower Infection Frequency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • R. W. Overell
    • 1
  • K. E. Weisser
    • 1
  • B. Hess
    • 1
  • R. Goodwin
    • 1
  • L. Clark
    • 1
  • K. H. Grabstein
    • 1
  1. 1.Immunex CorporationSeattleUSA

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