In Vitro Culture of a Primary Plasmacytoma that has Retained Its Dependence on Pristane Conditioned Microenvironment for Growth

Abstract

In BALB/cAn mice, intraperitoneal implantation of plastics (Merwin and Redmon 1963), paraffin oils or the pure alkane pristane (2,6,10,14 tetrermethylpentadecane), induces the formation of plasmacytomas (PCT) that arise in the peritoneal inflammatory tissues evoked by these agents (Potter and MacCardle 1964). The paraffin oils and pristane are known to induce the formation of a chronic oil granulomatous (OG) tissue on peritoneal surfaces (Potter and MacCardle 1964, Anderson et al 1985). This tissue contains predominantly macrophages and neutrophiles that have ingested or surrounded oil droplets and become fixed to peritoneal surfaces (Anderson et al 1985). The OG tissue is vascularized by angiogenesis from mesenteric blood vessels and covered by mesothelium. In addition, the OG tissue contains a rich supportive stroma of fibroblasts and reticular fibroblasts.