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Radioenzymatic Assays in Biological Fluids

  • Chapter
Histamine and Histamine Antagonists

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 97))

Abstract

The radioenzymatic assay of histamine (Snyder et al. 1966) provided a potentially specific and sensitive procedure for the measurement of histamine in small samples of biological fluids. A major advantage was that, unlike the biological or fluorometric assays, prior isolation of histamine from the sample was not required. The principle of the assay was simple. The sample was incubated with a radiolabeled methyl donor, S -adenosyl-L -methionine (SAMe) ([14C]- or [3H]methyl) and the enzyme histamine methyltransferase (EC 2.1.1.8) (HMT) in sodium phosphate buffer (pH 7.9) to convert the histamine in the sample to Nτ-[14C]- or [3H]methylhistamine. After the addition of NaOH, the radiolabeled methylhistamine was extracted from the reaction mixture into chloroform whereas the labeled donor and other reaction products remained in the aqueous phase. Because chloroform quenched the scintillation process the chloroform phase was evaporated to dryness and then scintillation cocktail was added for the assay of radioactivity.

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References

  • Almeida AP, Flye W, Deveraux D, Horakova Z, Beaven MA (1980) Distribution of histamine and histaminase (diamine oxidase) in blood of various species. Comp Biochem Physiol [C] 67C:187–190

    Article  CAS  Google Scholar 

  • Baxter JH, Beaven MA, Horakova Z (1974) Effects of adrenergic agents, theophylline and other drugs on dextran edema and histamine release in rats. Biochem Pharmacol 23:1211–1217

    Article  PubMed  CAS  Google Scholar 

  • Beaven MA, Cunha-Melo, JR (1988) Membrane phosphoinositide-activated signals in mast cells and basophils. Progr Allergy 42:123–184

    CAS  Google Scholar 

  • Beaven MA, Horakova Z (1978) The enzymatic isotopic assay of histamine. In: Roche e Silva M (ed) Histamines and antihistaminics. Spinger, Berlin Heidelberg New York, pp 151–173 (Handbook of experimental pharmacology, vol 18/2)

    Google Scholar 

  • Beaven, MA, Jacobsen S, Horakova Z (1972) Modification of the enzymatic isotopic assay of histamine and its application to measurement of histamine in tissues, serum and urine. Clin Chim Acta 37:91–103

    Article  PubMed  CAS  Google Scholar 

  • Beaven MA, Woldemussie E (1984) Histamine in body fluids: its measurements in different clinical states. New Engl Reg Allergy Proc 5:300–310

    Article  CAS  Google Scholar 

  • Beaven MA, Robinson-White A, Roderick NB, Kauffman GL (1982) The demonstration of histamine release in clinical conditions: a review of past and present assay procedures. Klin Wochenschr 60:873–881

    Article  PubMed  CAS  Google Scholar 

  • Beaven MA, Aiken DL, Woldemussie E, Soil AH (1983) Changes in histamine synthetic activity, histamine content and responsiveness to compound 40/80 with maturation of rat peritoneal mast cells. J Pharmacol Exp Ther 224:620–626

    PubMed  CAS  Google Scholar 

  • Bowsher RR, Verburg KM, Henry DP (1983) Rat histamine n-methyltransferase. Quantification, tissue distribution, purification, and immunologic properties J Biol Chem 258:12215–12220

    PubMed  CAS  Google Scholar 

  • Brown MJ, Ind PW, Barnes PJ, Jenner DA, Dollery CT (1980) A sensitive and specific radiometric method for the measurement of plasma histamine in normal individuals. Anal Biochem 109:142–146

    Article  PubMed  CAS  Google Scholar 

  • Correa FM, Saavedra JM (1985) Femtomole isotopic-enzymatic microassay of histamine in microgram amounts of brain tissue. Braz J Med Biol Res 18:409–414

    PubMed  Google Scholar 

  • Dent C, Nilam F, Smith IR (1979) Application of thin layer chromatography to histamine radioenzymatic assay (Proceedings). Agents Actions 9:34–35

    Article  PubMed  CAS  Google Scholar 

  • Dyer J, Warren K, Merlin S, Metcalfe DD, Kaliner M (1982) Measurement of plasma histamine: description of an improved method and normal values. J Allergy Clin Immunol 70:82–87

    Article  PubMed  CAS  Google Scholar 

  • Haimart M, Launay JM, Zurcher G, Cauet N, Dreux C, Da PR (1985) Simultaneous determination of histamine and n alpha-methylhistamine in biological samples by an improved enzymatic single isotope assay. Agents Actions 16:71–75

    Article  PubMed  CAS  Google Scholar 

  • Harvima RJ, Harvima LT, Fraki JE (1988) Optimization of histamine radio enzyme assay with purified histamine-n-methyltransferase. Clin Chim Acta 171:247–256

    Article  PubMed  CAS  Google Scholar 

  • Horakova Z, Beaven MA (1974) Time course of histamine release and edema formation in the rat paw after thermal injury. Eur J Pharmacol 27:305–312

    Article  PubMed  CAS  Google Scholar 

  • Horakova Z, Keiser HR, Beaven MA (1972) Blood and urine histamine levels in normal and pathological states as measured by a radiochemical assay. Clin Chim Acta 79:447–456

    Article  Google Scholar 

  • Kaplan AP, Beaven MA (1976) In vivo studies of the pathogenesis of cold urticaria, cholinergic urticaria, and vibration-induced swelling. J Invest Dermatol 67 327-332

    Article  PubMed  CAS  Google Scholar 

  • Kaplan AP, Gray L, Shaff RE, Horakova Z, Beaven MA (1975) In vivo studies of mediator release in cold urticaria and cholinergic urticaria. J Allergy Clin Immunol 55:394–402

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi Y, Maudsley DV (1972) A single-isotope enzyme assay for histamine. Anal Biochem 46:85–90

    Article  PubMed  CAS  Google Scholar 

  • Metzger WJ, Kaplan AP, Beaven MA, Trons JS, Patterson R (1976) Hereditary vibratory angioedema: confirmation of histamine release in a type of physical hypersenitivity. J Allergy Clin Immunol 57:605–608

    Article  PubMed  CAS  Google Scholar 

  • Miller RL, McCord C, Sanda M, Bourne HR, Melmon KL (1970) Application of the enzymatic double isotope dilution assay for the study of histamine in plasma. J Pharmacol Exp Ther 175:228–234

    PubMed  CAS  Google Scholar 

  • Moss J, Fahmy NR, Sunder N, Beaven MA (1981a) Hormonal and hemodynamic profile of an anaphylactic reaction in man. Circulation 63:210–213

    Article  PubMed  CAS  Google Scholar 

  • Moss J, Rosow CE, Savarese JJ, Philbin DM, Kniffen KJ (1986) Role of histamine in the hypotensive action of d-tubocurarine in humans. Anesthesiology 55:19–25

    Article  Google Scholar 

  • Rauls DO, Ting S, Lund M (1986) Analysis of plasma histamine: a modification of the enzymatic isotopic assay. J Allergy Clin Immunol 77:673–676

    Article  PubMed  CAS  Google Scholar 

  • Roberts LJ, Aulsebrook KA, Oates JA (1985) Comparative evaluation of the radioen-zymatic method for the determination of urinary histamine with a mass spec-trometric assay. J Chromatogr 338:41–49

    Article  PubMed  CAS  Google Scholar 

  • Robinson-White A, Beaven MA (1982) Presence of histamine and histamine-metabolizing enzyme in rat and guinea pig microvascular endothelial cells. J Pharmacol Exp Ther 223:440–445

    PubMed  CAS  Google Scholar 

  • Shaff RE, Beaven MA (1979) Increased sensitivity of the enzymatic isotopic assay of histamine: measurement of histamine in plasma and serum. Anal Biochem 94: 425–430

    Article  PubMed  CAS  Google Scholar 

  • Snyder SH, Baldessarini RJ, Axelrod J (1966) A sensitive and specific enzymatic isotopic assay for tissue histamine. J Pharmacol Exp Ther 153:544–549

    PubMed  CAS  Google Scholar 

  • Soll AH, Lewin K, Beaven MA (1979) Isolation of histamine-containing cells from canine fundic mucosa. Gastroenterology 77:1283–1290

    PubMed  CAS  Google Scholar 

  • Soll AH, Lewin KJ, Beaven MA (1981) Isolation of histamine-containing cells from rat gastric mucosa: biochemical and morphologic differences from mast cells. Gastroenterology 80:717–727

    PubMed  CAS  Google Scholar 

  • Taylor KM, Snyder SH (1971) Brain histamine: rapid apparent turnover altered by restraint and cold stress. Science 172:1037–1039

    Article  PubMed  CAS  Google Scholar 

  • Verburg KM, Bowsher RR, Henry DP (1983) A new radioenzymatic assay for histamine using purified histamine n-methyltransferase. Life Sci 32:2855–2867

    Article  PubMed  CAS  Google Scholar 

  • Verburg KM, Bowsher RR, Henry DP (1984) Kinetic analysis of the histamine n-methyltransferase reaction as used in the histamine radioenzymatic assay: optimization of assay specificity. Life Sci 35:241–251

    Article  PubMed  CAS  Google Scholar 

  • Warren K, Dyer J, Merlin S, Kaliner M (1983) Measurement of urinary histamine: comparison of fluorometric and radioisotopic-enzymatic assay procedures. J Allergy Clin Immunol 71:206–211

    Article  PubMed  CAS  Google Scholar 

Further Reading

  • Atkins PC, Valenzano M, Zweiman B (1982) Plasma concentrations of histamine measured by radioenzymatic assay: effects of histaminase incubations. J Allergy Clin Immunol 69:39–45

    Article  PubMed  CAS  Google Scholar 

  • Barnes PJ, Ind PW, Brown MJ (1982) Plasma histamine and catecholamines in stable asthmatic subjects. Clin Sci 62:661–665

    PubMed  CAS  Google Scholar 

  • Barnes PJ, Brown MJ, Dollery CT, Fuller RW, Heavey DJ, Ind PW (1986) Histamine is released from skin by substance P but does not act as the final vasodilator in the axon reflex. Br J Pharmacol 88:741–745

    PubMed  CAS  Google Scholar 

  • Blumenthal MN, Roitman B, Carlson G, Fish LA (1974) Isotope assay of histamine release. Biochem Med 11:312–317

    Article  PubMed  CAS  Google Scholar 

  • Brown JK, Frey MJ, Reed BR, Leff AR, Shields R, Gold WM (1984) Radioenzymatic assay for measurement of tissue concentrations of histamine: adaptation to correct for adherence of histamine to mechanical homogenizers. J Allergy Clin Immunol 73:473–478

    Article  PubMed  CAS  Google Scholar 

  • Brown MJ, Ind PW, Causon R, Lee TH (1982) A novel double-isotope technique for the enzymatic assay of plasma histamine: application to estimation of mast cell activation assessed by antigen challenge in asthamtics. J Allergy Clin Immunol 69:20–24

    Article  PubMed  CAS  Google Scholar 

  • Bruce C, Taylor WH, Westwood A (1979) An improved radioenzymatic assay for histamine in human plasma, whole blood, urine, and gastric juice. Ann Clin Biochem 16:259–264

    PubMed  CAS  Google Scholar 

  • Faraj BA, Gottlieb GR, Camp VM, Kutner M, Lolies P (1984) Development of a sensitive radioassay of histamine for in vitro allergy testing. J Nucl Med 25:56–63

    PubMed  CAS  Google Scholar 

  • Gleich GJ, Hull WM (1980) A Measurement of histamine: a quality control study. J Allergy Clin Immunol 66:295–298

    Article  PubMed  CAS  Google Scholar 

  • Guilloux L, Hartmann D, Ville GA (1981) Enzymatic isotopic assay for human plasma histamine. Clin Chim Acta 116:269–275

    Article  PubMed  CAS  Google Scholar 

  • Harvima RJ, Neittaanmaki H, Harvima IT, Kajander EO, Fraki JE (1984) Histamine n-methyltransferase preparation used for histamine assay contains endogenous histamine. Clin Chim Acta 143:337–341

    Article  PubMed  CAS  Google Scholar 

  • Heavey DJ, Ind PW, Miyatake A, Brown MJ, Macdermot J, Dollery CT (1984) Histamine released locally after intradermal antigen challenge in man. Br J Clin Pharmacol 18:915–919

    PubMed  CAS  Google Scholar 

  • Heavey DJ, Kobza-Black A, Barrow SE, Chappell CG, Greaves MW, Dollery CT (1986) Prostaglandin D2 and histamine release in cold urticaria. J Allergy Clin Immunol 78:458–461

    Article  PubMed  CAS  Google Scholar 

  • Ind PW, Miyatake A, Heavey DJ, Dollery C (1984) Local histamine release after immunological and non-immunological mast cell degranulation in vivo. Agents Actions 14:417–419

    Article  PubMed  CAS  Google Scholar 

  • Ind PW, Barnes PJ, Brown MJ, Dollery C (1985) Plasma histamine concentration during propranolol induced bronchoconstriction. Thorax 40:903–909

    Article  PubMed  CAS  Google Scholar 

  • Levy DA, Widra M (1973) A microassay for studying allergic histamine release from human leukocytes using an enzymic-isotopic assay for histamine. J Lab Clin Med 81:291–297

    PubMed  CAS  Google Scholar 

  • Morgan DJ, Moodley I, Philips MJ, Davies R (1983) Plasma histamine in asthmatic and control subjects following exercise: influence of circulating basophils and different assay techniques. Thorax 38:771–777

    Article  PubMed  CAS  Google Scholar 

  • Morita Y, Matsumoto Y, Miyamoto T, Horiuchi Y (1978) A clinical study of concanavalin A-induced histamine release utilizing a single isotopic enzymatic assay of histamine. Ann Allergy 40:47–50

    PubMed  CAS  Google Scholar 

  • Roberts LJ, Sweetman BJ, Lewis RA, Austen KF, Oates JA (1980) Increased production of prostaglandin D2 in patients with systemic mastocytosis. N Engl J Med 306:1400–1404

    Article  Google Scholar 

  • Salberg DJ, Hough LB, Kaplan DE, Domino EA (1977) Reverse double-isotope enzymatic histamine assay: advantages over single-isotope methods. Life Sci 21:1439–1446

    Article  PubMed  CAS  Google Scholar 

  • Sheffer AL, Austen KF (1984) Exercise-induced anaphylaxis. J Allergy Clin immunol 73:699–703

    Article  PubMed  CAS  Google Scholar 

  • Sheffer AL, Soter NA, McFadden ER, Austen KF, (1983) Exercise-induced anaphylaxis: a distinct form of physical allergy. J Allergy Clin Immunol 71:311–316

    Article  PubMed  CAS  Google Scholar 

  • Sheffer AL, Tong AK, Murphy GF, Lewis RA, McFadden ER, Austen KF (1985) Exercise-induced anaphylaxis: a serious form of physical allergy associated with mast cell degranulation. J Allergy Clin Immunol 75:479–484

    Article  PubMed  CAS  Google Scholar 

  • Soter NA, Wasserman SI, Austen KF (1976) Cold urticaria: release into the circulation of histamine and eosinophil chemotactic factor of anaphylaxis during cold challenge. N Engl J Med 294:687–690

    Article  PubMed  CAS  Google Scholar 

  • Soter ΝΑ, Wasserman SI, Austen KF, McFadden ER (1980) Release of mast-cell mediators and alterations in lung function in patients with cholinergic urticaria. N Engl J Med 302:604–608

    Article  PubMed  CAS  Google Scholar 

  • Subramanian N, Mitznegg P, Domschke W (1978) A rapid and sensitive enzymatic-isotopic method for routine assay of histamine in blood, plasma and biopsy tissue: reevaluation. Acta Hepatogastroenterol (Stuttg) 25:456–458

    CAS  Google Scholar 

  • Yamatodani A, Fukuda H, Wada H, Iwaeda T, Watanabe T (1985) High-performance liquid chromatographic determination of plasma and brain histamine without previous purification of biological samples: cation-exchange chromatography coupled with post-column derivatization fluorometry. J Chromatogr 344:115–123

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. M. A. Beaven
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Editors and Affiliations

  1. Department of Pharmacology, Karolinska Institutet, Box 60400, S-104 01, Stockholm, Sweden

    Börje Uvnäs M.D.

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© 1991 Springer-Verlag Berlin Heidelberg

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Beaven, M.A. (1991). Radioenzymatic Assays in Biological Fluids. In: Uvnäs, B. (eds) Histamine and Histamine Antagonists. Handbook of Experimental Pharmacology, vol 97. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75840-9_5

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  • DOI: https://doi.org/10.1007/978-3-642-75840-9_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75842-3

  • Online ISBN: 978-3-642-75840-9

  • eBook Packages: Springer Book Archive

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