Nitrite Reduction in The Roots And Leaves of Pisum Sativum

  • D. P. Hucklesby
  • M. J. Emes
  • C. G. Bowsher
  • R. Cammack

Abstract

The location of the nitrate/nitrite reducing mechanisms in plants is distributed between the root and the shoot in proportions which vary according to species. At one extreme, nitrate may be reduced almost entirely in the shoot, e.g. Beta and Chenopodium, while at the other extreme most reduction occurs in the root system as in some woody species. In the majority of species, however, including many important crop plants, both root and shoot participate in reduction. This subject has been discussed in some detail (Pate 1973). The mechanisms of function of both nitrate and nitrite reduction are better understood in leaves than roots. Root nitrogen assimilation has been reviewed by Oaks and Hirel (1985). Shortly after methods became available for the extraction and assay of nitrite reductase (Hageman et al. 1962), the enzyme was shown to be active in tomato roots (Sanderson and Cocking 1964). A few years previously an interesting study by Butt and Beevers (1961), approaching the subject from the direction of carbon-nitrogen relations had shown that glucose-6-phosphate could increase the rate of disappearance of nitrite in contact with preparations from maize roots. The association of both nitrite reductase and the enzymes of the pentosephosphate pathway (PPP) with root plastids, has been developed in a number of studies in the intervening years (see, e.g. Emes and Fowler 1979 a,b). The present paper describes a dual approach to the subject of nitrite reduction by roots, in which studies were made of the purified nitrite reductase (NIR) enzyme and of carbohydratenitrite relationships as found in root plastids. Pea was chosen for this work as a species in which nitrate was thought to be reduced in roots, and from which well authenticated root plastids can be extracted. A large number of labelling techniques was used in this study. Details of these may be found in Bowsher et al. (1988,1989).

Keywords

Nitrite Assimilation NADPH Dehydrogen NADP 

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References

  1. Aparicio PJ, Knaff DB, Malkin R (1975) The role of an iron-sulphur centre and siroheme in spinach nitrite reductase. Arch Biochem Biophys 169: 102–107PubMedCrossRefGoogle Scholar
  2. Bowsher CG, Emes MJ, Cammack R, Hucklesby DP (1988) Purification and properties of nitrite reductase from roots of pea. Planta 175: 334–340CrossRefGoogle Scholar
  3. Bowsher CG, Hucklesby DP, Emes MJ (1989) Nitrite reduction and carbohydrate metabolism in plastids purified from roots of Pisum sativum L. Planta 177: 359–366CrossRefGoogle Scholar
  4. Butt VS, Beevers H (1961) The regulation of pathways of glucose metabolism in maize roots. Biochem J 80: 21–27PubMedGoogle Scholar
  5. Cammack R, Hucklesby DP, Hewitt EJ (1978) Electron paramagnetic resonance studies of the mechanism of leaf nitrite reductase. Signals from the iron-sulphur centre and haem under turnover conditions. Biochem J 171: 519–536PubMedGoogle Scholar
  6. Dalling MJ, Hucklesby DP, Hageman RH (1973) A comparison of nitrite reductase enzymes from green leaves, scutella and roots of corn (Zea mays L.). Plant Physiol 51: 481–484PubMedCrossRefGoogle Scholar
  7. Hageman RH, Cresswell CF, Hewitt EJ (1962) Reduction of nitrate, nitrite and hydroxylamine to ammonia by enzymes extracted from higher plants. Nature 193: 247–250PubMedCrossRefGoogle Scholar
  8. Hucklesby DP, Dalling MJ, Hageman RH (1972) Some properties of two forms of nitrite reductase from corn (Zea mays L.) scutellum. Planta 104: 220–233CrossRefGoogle Scholar
  9. Ida S, Mori E, Morita Y (1974) Purification, stabilization and characterization of nitrite reductase from barley roots. Planta 121: 213–224CrossRefGoogle Scholar
  10. Nagaoka S, Hirasawa M, Fukushima K, Tamura G (1984) Methyl viologen-linked nitrite reductase from bean roots. Agricultural Biol Chem 48: 1179–1188CrossRefGoogle Scholar
  11. Ninomiya Y, Sato S (1984) A ferredoxin-like electron-carrier from non-green cultured tobacco cells. Plant Cell Physiol 25: 453–458Google Scholar
  12. Oaks A, Hirel B (1985) Nitrogen metabolism in roots. Ann Rev Plant Physiol 36: 345–364CrossRefGoogle Scholar
  13. Pate JS (1973) Uptake, assimilation and transport of nitrogen compounds by plants. Soil Biol Biochem 5: 109–119CrossRefGoogle Scholar
  14. Sanderson GW, Cocking EC (1964) Enzymic assimilation of nitrate in tomato plants I. Reduction of nitrate to nitrite. Plant Physiol 39: 416–422PubMedCrossRefGoogle Scholar
  15. Suzuki A, Jacquot JP, Vidal J, Oaks A (1985) An electron-transport system in maize roots for reactions of glutamate synthase and nitrite reductase. Physiological and immunological properties of the electron carrier and pyridine nucleotide reductase. Plant Physiol 78: 374–378PubMedCrossRefGoogle Scholar
  16. Wada K, Onda M, Matsubara H (1986) Ferredoxin isolated from plant non-photosynthetic tissues. Purification and characterization. Plant Cell Physiol 27: 407–415Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • D. P. Hucklesby
    • 1
  • M. J. Emes
    • 2
  • C. G. Bowsher
    • 3
  • R. Cammack
    • 4
  1. 1.Department of Molecular BiologyLong Ashton Research StationLong Ashton, BristolUK
  2. 2.School of Biological SciencesUniversity of ManchesterManchesterUK
  3. 3.Dept. of Molecular StudiesUniversity of GuelphGuelphCanada
  4. 4.Kings CollegeUniversity of LondonLondonUK

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