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Cytotoxicity Tests in the Biological Evaluation of Medical Devices

  • Wolfgang G. K. Müller-Lierheim
Conference paper

Abstract

Since the early sixties mammalian and human cell cultures have been used as sensitive tool in the biological evaluation of medical and dental materials and devices. The three main areas of interest for the application of cell culture tests are:
  • cytotoxicity tests

  • mutagenicity tests, and

  • cell models for studying the interaction between device surface and surrounding tissue.

Cytotoxicity tests are frequently used in the development, preclinical testing and quality assurance and are now subject to international standards. Based on the experience derived from testing more than 1000 medical and dental devices over a period of nine years some general remarks about cytotoxicity testing and examples of cytotoxicity test protocols are given.

Keywords

Primary Cell Culture Cytotoxicity Test Dental Material Biological Endpoint Optical Absorption Measurement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature

  1. (1).
    W. L. Guess, S.A. Rosenbluth, B. Schmidt, and J. Autian, “Agar Diffusion Method for Toxicity Screening of Plastics on Cultured Cell Monolayers”, J. Pharm. Sci., 54, 1545–1547 (1965)CrossRefGoogle Scholar
  2. (2).
    Elwood O. Dillingham, “Primary Acute Toxicity Screen for Biomaterials: Rationale, In Vitro/In Vivo Relationship and Interlaboratory Performance”, in: Cell-Culture Test Methods, ASTM Special Technical Publication 810, pp. 51–70, S.A. Brown ed., Philadelphia 1983.CrossRefGoogle Scholar
  3. (3).
    O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R.J. Randall, “Protein Measurement with Folin Phenol Reagent”, J. Biol. Chem. 193, 265–275 (1951)Google Scholar
  4. (4).
    S. Roßberger, “Standardverfahren für die Zytotoxizitätsprüfung: Der MTT-Test”, Z. Zahnärztl. Implantol. IV, 251–253 (1988)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Wolfgang G. K. Müller-Lierheim
    • 1
  1. 1.Biological LaboratoriesDr. Müller-Lierheim AGPlaneggGermany

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