In Vitro Evaluation of the Effects of Macrophages and Synthetic Materials on the Fibroblast Proliferation and Collagen Synthesis

  • Jeong OK Lim
  • C. C. Chu
  • Max Appel


The essential role of macrophages in the biological defense system has been well illustrated in many studies using foreign materials of either biological or synthetic nature.1 – 5 The presence of synthetic foreign materials whether because of necessity like modern surgical devices for the reconstruction of injured or diseased tissues/organs (e.g., vascular grafts, surgical mesh fabrics, artificial knee joint, etc.), or because of incidences like silica asbestos particles, eventually lead to a chronic inflammatory response followed by fibrous capsule formation around the synthetic foreign materials. A recent study of the ultra-structure of large accumulated cells immediately adjacent to synthetic foreign materials (i.e., a biodegradable wound closure material) indicated that macrophages played a major role in the phagocytosis of wound debris, and subsequent degradation, invasion, and phagocytosis of the synthetic foreign material.6


Tissue Culture Plate Polypropylene Mesh Fibroblast Proliferation Synthetic Substrate Synthetic Biomaterial 
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  1. 1.
    Bitterman PB, Rennard SK, Hunninghake GW, Crystal RG (1982) Human alveolar macrophage growth factor for fibroblasts Regulation and partial characterization, J Clin Invest, 70 (4): 806–22CrossRefGoogle Scholar
  2. 2.
    Wahl SM, Wahl LM, McCarthy JB, Chedid L (1979) Macrophage activation by mycobacterial water soluble compounds and synthetic muramyl dipeptide, J Immunol, 122 (6): 2226–31Google Scholar
  3. 3.
    Heppleston AG, Styles JA (1967) Activity of a macrophage factor in collagen formation by silica, Nature, 214: 521–522CrossRefGoogle Scholar
  4. 4.
    Anderson JM, Miller KM (1984) Biomaterial biocompatibility and the macrophage, Biomaterials, 5 (1): 5–10CrossRefGoogle Scholar
  5. 5.
    Van Furth R (1975) Mononuclear Phagocytes in Immunity, Infection, and Pathology, Blackwell Sci Publ, OxfordGoogle Scholar
  6. 6.
    Matlaya BJ, Salthouse TN (1983) Ultrastructural observations of cells at the interface of a biodegradable polymer: Polyglactin 910, J Biomed Mater Res, 17 (1): 185–97CrossRefGoogle Scholar
  7. 7.
    Miller K, Handfield RI, Kagan E (1978) The effect of different mineral dusts on the mechanism of phagocytosis: A scanning electron microscope study, Environ Res, 15, 139–154CrossRefGoogle Scholar
  8. 8.
    Leake ES, Wright MJ (1979) Variations on the form of attachment of rabbit alveolar macrophages to various substrata as observed by scanning electron microscopy, J Reticuloendothel Soc, 25, 417–441Google Scholar
  9. 9.
    Rasp FL, Clawson CC, Hoidal JR, and Repine JE (1979) Quantitation and scanning electron microscopic comparison of human alveolar macrophage and polymorphonuclear leukocyte adherence to nylon fibers in vitro, J Reticuloendothel Soc, 25, 101–109Google Scholar
  10. 10.
    Berhard B, Manolescu N, Simonescu N, Ciocnitu V (1983) Changes concerning the external structure of peritoneal macrophages, due to the DQ-12 standard dust, Environ Res, 31, 256–265CrossRefGoogle Scholar
  11. 11.
    Leake ES, Wright MJ, Myrvik QN (1975) Differences in surface morphology of alveolar macrophages attached to glass and to millipore filters: A scanning electron microscope study, J Reticuloendothel Soc, 17, 370–379Google Scholar
  12. 12.
    Spilizewski KL, Marchant RE, Anderson JM, Hiltner A (1987) In vivo leukocyte interactions with the NHLBI-DTB primary reference materials: polyethylene and silica-free polydimethylsiloxane, Biomaterials, 8, 12–17CrossRefGoogle Scholar
  13. 13.
    Sipe JD (1985) Interleukin 1 as a key factor in the acute-phase response, in The Acute-Phase Response to Injury and Infection, Chapter 2, Gordon and Koj (Eds), Elsevier Science Publishers, The Netherlands, 23–35Google Scholar
  14. 14.
    Dinarello CA (1984) Interleukin 1, Rev Infect Dis, 6, 51–95CrossRefGoogle Scholar
  15. 15.
    Leibovich SJ, Ross RA (1976) A macrophage dependent factor that stimulates the proliferation of fibroblasts in vitro, Am J Pathol, 84 (3): 501–14Google Scholar
  16. 16.
    Martin BM, Gimbrone MA, Unanue ER, Cotran RS (1981) Stimulation of nonlymphoid mesenchymal cell proliferation by a macrophage-derived growth factor, J Immunol, 126 (4): 1510–5Google Scholar
  17. 17.
    Schmidt JA, Mizel SB, Cohen D, Green I (1982) Interleukin 1, a potential regulator of fibroblast proliferation, J Immunol, 128 (5): 2177–82Google Scholar
  18. 18.
    Klaus GGB (1987) Lymphocytes - A Practical Approach, IRL Press, Washington, DC, pp 211–212Google Scholar
  19. 19.
    Postlethwaite AE, Lachman LB, Mainardi CL, Kang AH (1983) Interleukin 1 stimulation of collagenase production by cultured fibroblasts, J Exp Med, 157 (2): 801–6CrossRefGoogle Scholar
  20. 20.
    Besedovsky HO, del Ray A, Sorkin E, Dinarello CA (1986) Immunoregulatory Feedback Between Interleukin-1 and Glucocorticoid Hormones, Science 233: 652–654CrossRefGoogle Scholar
  21. 21.
    Gery I (1982) Production and Assay of Interleukin-1 (IL-1), In: Isolation, Characterization, and Utilization of T Lymphocyte Clones, Academic Press, p 41Google Scholar
  22. 22.
    Kenney JS, Masada MP, Eugui EM, Delustro BM, Mulkins MA, Allison AC (1987) Monoclonal Antibodies to Human Recombinant Interleukin 1 (IL 1): Quantitation of IL 1 and Inhibition of Biological Activity, J Immunol, 138: 4236Google Scholar
  23. 23.
    Spencer H (1977) Pathology of the Lung, W.B. Sanders, PhiladelphiaGoogle Scholar
  24. 24.
    Bitterman PB, Adelberg S, Crystal RG (1983) Mechanisms of pulmonary fibrosis spontaneous release of the alveolar macrophage derived growth factor in the interstitial lung disorders, J Clin Invest, 72 (5): 1801–13CrossRefGoogle Scholar
  25. 25.
    Lemaire I, Beaudoin H, Masse S, Grondin C (1986) Alveolar macrophage stimulation of lung fibroblast growth in asbestos - included pulmonary fibrosis, Am J Pathol, 122 (2): 205–11Google Scholar
  26. 26.
    Miller KM, Anderson JM (1988) Human monocyte/macrophage activation and Interleukin 1 generation by biomedical polymers, J Biomed Mater Res, 22: 713CrossRefGoogle Scholar
  27. 27.
    Stea S, Ciapetti G, Pizzoferrato A (1984) The release of macrophage-dependent fibroblast stimulating activity (MFS) from macrophages treated with powdered biomaterials, Biointeractions - Materials/Interactions, City University, London, January 4–6, p 11Google Scholar
  28. 28.
    Gregg S, Sing K (1967) Adsorption, Surface Area and Porosity, Academic Press, New YorkGoogle Scholar
  29. 29.
    Bergman I, Loxley R (1963) Two improved and simplified methods for the spectrophotometric determination of hydroxyproline, Analytic Chem, 35 (12): 1961–65CrossRefGoogle Scholar
  30. 30.
    Switzer BR, Summer GK (1971) Improved method for hydroxyproline analysis in tissue hydrolyzates, Analytical Biochem, 39: 487CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Jeong OK Lim
    • 1
  • C. C. Chu
    • 1
  • Max Appel
    • 1
  1. 1.Department of Textiles & Apparel, Martha Van Rensselaer HallCornell UniversityIthacaUSA

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