Helicobacter pylori Hemagglutinins

  • T. Wadström


Studies in the past decade on a number of mucosal surface microbial pathogens have shown that they usually colonize mucosal surfaces by binding to glycoconjugate receptors in the mucin layer of epithelial cells (Wadström et al. 1985). Different bacterial surface proteins (adhesins) or hair-like surface appendages (fimbriae or pili) as well as nonfimbrial adhesins have been reported to be involved in such colonization processes. Pioneering studies by Duguid and Old (1980) showed that many fimbriae of enteric pathogens hemagglutinate erythrocytes of various animal species and can be classified as bacterial surface hemagglutinins or bacterial surface lectins (Wadström and Trust 1984). Despite the great interest in how Helicobacter pylori specifically colonizes human gastric epithelium, very few studies have dealt with how this pathogen penetrates the gastric mucus layer, and penetrates into this epithelium to get access to submucosal tissues. Emödy et al. (1988), Evans et al. (1988) and recently also Nakazawa et al. (1989) have reported on the hemagglutination properties of H. pylori. We have also recently characterized a sialic-acid-specific hemagglutinin of H. pylori (Carlsson et al. 1989), and our data on hemagglutination profiles suggest the presence of two more hemagglutinins in H. pylori.


Surface Hydrophobicity Pylorus Strain Cell Surface Hydrophobicity Hydrophobic Interaction Chromatography Gastric Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Albertsson P-A (1978) Partition between polymer phases. J Chromatogr 159:111–122CrossRefGoogle Scholar
  2. Bode G, Malfertheiner P, Ditschuneit P (1988) Pathogenic implications of ultrastructural findings; Campylobacter pylori related to gastrointestinal disease. Scand J Gastroenterol 23: suppl 142:25–39CrossRefGoogle Scholar
  3. Booth BA, Sciono CV, Finkelstein RA (1986) Adhesins of Vibrio cholerae. In: Mirelman D (ed) Microbial lectins and agglutinins. Wiley, New York, pp:169–182Google Scholar
  4. Carlsson Å, Emödy L, Ljungh Å, Wadström T (1989) Carbohydrate receptor specificity of haemagglutinins of Campylobacter pylori. In: Megraud F, Lamonliatte H (eds) Gastroduodenal pathology and Campylobacter pylori. Excerpta Medica Int Congress Series Nr 847, Elsevier, Amsterdam, pp:375–378Google Scholar
  5. Duguid J, Old DC (1980) Adhesive properties of Enterobacteriaceae. In: Beachey E (ed) Bacterial adherence. Receptors and recognition. Chapman and Hall, pp:186–217Google Scholar
  6. Emödy L, Carlsson Å, Ljungh Å, Wadström T (1988) Mannose resistant haemagglutination of Campylobacter pylori. Scand J Infect Dis 20:353–354PubMedCrossRefGoogle Scholar
  7. Evans DJ Jr, Moulds JJ, Graham DY (1988) N-Acetylneuraminyllactose-binding fibrillar haemagglutinin of Campylobacter pylori: a putative colonization factor antigen. Infect Immun 56:2896–2906PubMedGoogle Scholar
  8. Gonzalez EA, Blanco J, Baloda S, Wadström T (1988) Relative cell surface hydrophobicity of Escherichia coli strains with various recognized fimbrial antigens and without recognized fimbriae. Bact Hyg 269:218–236Google Scholar
  9. Hazell SL, Lee A, Brady L, Hennesey W (1986) Campylobacter and gastritis association with intracellular species and adaption to an environment of mucus as important facts in colonization of gastric epithelium. J Infect Dis 153:658–663PubMedCrossRefGoogle Scholar
  10. Höök M, Switalski L, Wadström T, Lindberg M (1988) Interactions of pathogenic microorganisms with fibronectin. In: Mosher P (ed) Fibronectin. Academic, New York, pp:295–308Google Scholar
  11. Karlsson KA (1989) Animal glycosphingolipids as membrane attachment sites to bacteria. Annu Rev Biochem 58:309–350PubMedCrossRefGoogle Scholar
  12. Knutton S, Baldwin T, Williams PH, McNeish AS (1989) Actin accumulation at sites of bacterial adhesion to tissue culture cells: the basis of a new diagnostic test for enteropathogenic and enterohemmorhagic Escherichia coli. Infect Immun 57:1290–1298PubMedGoogle Scholar
  13. Labigne-Rousell A, Falkow S (1988) Distribution and degree of heterogenity of the afimbrialadhesin-encoding operon (afa) among pathogenic Escherichia coli. Infect Immun 56:640–648Google Scholar
  14. Lichtenberger LM (1987) Membranes and barriers: With a focus on the gastric mucosal barrier. Clin Invest Med 10:181–188PubMedGoogle Scholar
  15. Lindahl M, Brossmer R, Wadström T (1987) Carbohydrate receptors specific to K99 fimbriae of enterotoxigenic Escherichia coli. Glycoconjugate J 4:51–58CrossRefGoogle Scholar
  16. Lindahl M, Faris A, Wadström T, Hjerten S (1981) A new test based on “salting out” to measure relative surface hydrophobicity of bacterial cells. Biochim Biophys Acad 677:471–476CrossRefGoogle Scholar
  17. Lingwood CA, Law H, Pellizzari A, Sherman P, Drumm B (1989) Gastric glycerolipid as a receptor for Campylobacter pylori. Lancet II:238–241CrossRefGoogle Scholar
  18. Nakazawa T, Kennishi H, Takemoto P, Shigeda M, Kochiyama T (1989) Haemagglutination properties of Campylobacter pylori. In Takemoto T, Kawai K, Shimoyama T (eds) Campylobacter pylori and gastrointestinal diseases. Tokyo International Symposium on Campylobacter pylori, Taisho Pharmaceutical Co, pp:87–91Google Scholar
  19. Qadri F, Hossain SA, Cizar I, Haider K, Ljungh Å, Wadström T, Sack DA (1988) Congo red binding and salt agglutination as indicators of virulence in Shigella species. J Clin Microbiol 26:1343–1348PubMedGoogle Scholar
  20. Sjöberg PO, Lindahl M, Fröman G, Porath J, Wadström T (1988) Isolation and characterization of CS 2 sialic acid specific lectons of enterotoxigenic Escherichia coli. Biochem J 255:105–111PubMedGoogle Scholar
  21. Wadström T (1987) Aeromonas and Plesiomonas-enteric infections and fecal carriage. Experientia 43:362–364CrossRefGoogle Scholar
  22. Wadström T (1988) Adherence traits and mechanisms of microbiological adhesion in the gut. Bailleies Clin Trop Med Commun Dis 3:417–433Google Scholar
  23. Wadström T, Aleljung P (1989) Molecular mechanisms of bowel colonization. In: Midvedt T, Grubb R, and Nolin E (eds) “The regulative and protective role of the normal microflora”. Macmillan, Houndsmills, London, pp:35–46Google Scholar
  24. Wadström T, Baloda SB (1986) Molecular aspects on small bowel colonization by enterotoxigenic Escherichia coli. Microeco Ther 16:243–255Google Scholar
  25. Wadström T, Sjöberg P-O, Lindahl M (1984) Sialic acid specific lectins of enterotoxigenic Escherichia coli. In: Bog-Hansen TC, Breborowicz (eds) Lectins, vol 4, de Gruyter, Berlin, pp:417–423Google Scholar
  26. Wadström T, Switalski LM, Speziale P, Rubin K, Ryden C, Fröman G, Faris A, Lindberg M, Hökk M (1985) Binding of microbial pathogens to connective tissue fibronectin: an early step in the localized and invasive infections. In: Jackson GG, Thomas H (eds) Bayer-Symposium VIII, Springer, Berlin Heidelberg New York, pp:193–207Google Scholar
  27. Wadström T, Trust TJ (1984) Bacterial surface lectins. In: Jeljaszewicz J, Easman CSF (eds) Medical microbiology, vol 4. Academic, London, pp:287–334Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • T. Wadström

There are no affiliations available

Personalised recommendations