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Urease and Catalase as Virulence Factors of Helicobacter pylori

  • Conference paper
Helicobacter pylori 1990

Abstract

Since the first report of the urease activity of Helicobacter pylori [1], this enzyme has captured a large amount of attention from researchers interested in the detection and pathogenicity of this gastric bacterium. This interest is in part aroused by the extraordinary urease activity seen in H. pylori [2–5]. Another enzyme of H. pylori noted for its vigor is catalase [6]. While not the subject of intense investigation to date, this latter enzyme may be as important to H. pylori as urease with respect to virulence and pathogenicity.

Stuart Hazell is a H.J. Martin Fellow supported by the National Health and Medical Research Council of Australia. This work was supported in part by Veterans Affairs and in part by grant DK- 39919 from the National Institute of Digestive and Kidney Diseases

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References

  1. Langenberg ML, Tytgat GN, Schipper MEI, Rietra PJGM, Zanen HC (1984) Campylobacter- like organisms in the stomach of patients and healthy individuals. Lancet I:1348

    Article  Google Scholar 

  2. Owen RJ, Martin SR, Borman P (1985) Rapid urea hydrolysis by gastric Campylobacters. Lancet I:111

    Article  Google Scholar 

  3. Hazell SL, Lee A, Brady L, Hennessy W (1986) Campylobacter pyloridis and gastritis: association with intercellular spaces and adaption to an environment of mucus as important factors in colonization of the gastric epithelium. J Infect Dis 153:658–663

    Article  PubMed  CAS  Google Scholar 

  4. Mobley HLT, Cortesia MJ, Rosenthal LE, Jones BD (1988) Characterization of urease from Campylobacter pylori. J Clin Microbiol 26:831–836

    PubMed  CAS  Google Scholar 

  5. Ferrero RL, Hazell SL, Lee A (1988) The urease enzymes of Campylobacter pylori and a related bacterium. J Med Microbiol 27:33–40

    Article  PubMed  CAS  Google Scholar 

  6. Marshall BJ, Royce H, Annear DI, Goodwin CS, Pearman JW, Warren JR, Armstrong JA (1984) Original isolation of Campylobacter pyloridis from human gastric mucosa. FEMS Microbios Lett 25:83–88

    Google Scholar 

  7. Taylor MB, Goodwin CS, Karim QN (1988) Two urease activities with different pH optima in Campylobacter pylori and similar organisms. FEMS Microbiol Lett 55:259–262

    Article  CAS  Google Scholar 

  8. Taylor MB, Karim QN (1988) Urease activity of Campylobacter pylori. J Clin Pathol 41:112

    Article  PubMed  CAS  Google Scholar 

  9. McNulty CAM, Wise R (1985) Rapid diagnosis of Campylobacter-associated gastritis. Lancet 1:1443–1444

    Article  PubMed  CAS  Google Scholar 

  10. Marshall BJ, Warren JR, Francis GJ, Langton SR, Goodwin CS, Blincow ED (1987) Rapid urease test in the management of Campylobacter pyloridis-associated gastritis. Am J Gastroenterol 82:200–210

    PubMed  CAS  Google Scholar 

  11. Hazell SL, Borody TJ, Gal A, Lee A (1987) Campylobacter pyloridis gastritis I: detection of urease as a marker of bacterial colonization and gastritis. Am J Gastroenterol 82:292–296

    PubMed  CAS  Google Scholar 

  12. Graham DY, Klein PD, Evans DJ, Evans DG, Alpert LC, Opekun AR, Boutton TW (1987) Campylobacter pyloridis detected noninvasively by the 13C-urea breath test. Lancet I:1174–1177

    Article  Google Scholar 

  13. Marshall BJ, Surveyor I (1988) Carbon-14 urea breath test for the diagnosis of Campylobacter pylori associated gastritis. J Nucl Med 29:11–16

    PubMed  CAS  Google Scholar 

  14. Evans DJ Jr, Evans DG, Graham DY, Klein PD (1989) A sensitive and specific serologic test for detection of Campylobacter pylori infection. Gastroenterology 96:1004–1008

    PubMed  Google Scholar 

  15. Dent JC, McNulty CAM, Uff JS, Gear MWL, Wilkinson SP (1988) Campylobacter pylori urease: a new serological test. Lancet I:1002

    Article  Google Scholar 

  16. Mobley HLT, Haussinger RP (1989) Microbial ureases: significance, regulation, and molecular characterization. Microbiol Rev 53:85–108

    PubMed  CAS  Google Scholar 

  17. Wallace RJ, Cheng K-J, Dinsdale D, Orskov ER (1979) An independent microbial flora of the epithelium and its role in the ecomicrobiology of the rumen. Nature 279:424–426

    Article  PubMed  CAS  Google Scholar 

  18. Javorsky P, Rybosova E, Havassy I, Horsky K, Kmet V (1987) Urease activity of adherent bacteria and rumen fluid bacteria. Physiol Bohemoslov 36:75–81

    PubMed  CAS  Google Scholar 

  19. Bode G, Malfertheiner P, Nilius M, Lehnhardt G, Ditschuneit H (1989) Ultrastructural localisation of urease in outer membrane and periplasm of Campylobacter pylori. J Clin Pathol 42:778–779

    Article  PubMed  CAS  Google Scholar 

  20. Gatermann S, John J, Marre R (1989) Staphylococcus saprophyticus urease: characterization and contribution to uropathogenicity in unobstructed urinary tract infection of rats. Infect Immun 57:110–116

    PubMed  CAS  Google Scholar 

  21. Nickel JC, Olson M, McLean RJ, Grant SK, Costerton JW (1987) An ecological study of infected urinary stone genesis in an animal model. Br J Urol 59:21–30

    Article  PubMed  CAS  Google Scholar 

  22. McLean RJ, Nickel JC, Cheng KJ, Costerton JW (1988) The ecology and pathogenicity of urease producing bacteria in the urinary tract. CRC Crit Rev Microbiol 16:37–79

    Article  CAS  Google Scholar 

  23. Goodwin CS, Armstrong JA, Marshall BJ (1986) Campylobacter pyloridis, gastritis, and peptic ulceration. J Clin Pathol 39:353–365

    Article  PubMed  CAS  Google Scholar 

  24. Korff RW, Glick D (1951) Role of urease in the gastric mucosa II: in vitro studies with isotopic urea on frog mucosa. Am J Physiol 165:688–694

    Google Scholar 

  25. Bronsdon MA, Schoenknecht FD (1988) Campylobacter pylori isolated from the stomach of the monkey, Macaca nemestrina. J Clin Microbiol 26:1725–1728

    PubMed  CAS  Google Scholar 

  26. Baskerville A, Newell DG (1988) Naturally occurring chronic gastritis and H. pylori infection in the rhesus monkey: a potential model for gastritis in man. Gut 29:465–472

    Article  PubMed  CAS  Google Scholar 

  27. Lee A, Hazell SL, O’Rourke J, Kouprach S (1988) Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun 56:2843–2850

    PubMed  CAS  Google Scholar 

  28. Lee A, Hazell SL (1988) Campylobacter pylori in health and disease: an ecological perspective. Microb Ecology Health Dis 1:1–16

    Article  Google Scholar 

  29. Phillips MW, Lee A, Leach WD (1978) The mucosa associated microflora of the rat intestine: a study of normal distribution and magnesium sulphate induced diarrhoea. Aust J Exp Biol Med Sci 56:649–662

    Article  PubMed  CAS  Google Scholar 

  30. Casiano-Colon A, Marquis RE (1988) Role of the arginine deiminase system in protecting oral bacteria and an enzymatic basis for acid tolerance. Appl Environ Microbiol 54:1318–1324

    PubMed  CAS  Google Scholar 

  31. MacNab RM, Castle AM (1987) A variable stoichiometry model for pH homeostasis in bacteria. Biophys J 52:637–647

    Article  PubMed  CAS  Google Scholar 

  32. Marquis RE, Bender GR, Murray DR, Wong A (1987) Arginine deiminase system and bacterial adaptation to acid environments. Appl Environ Microbiol 53:198–200

    PubMed  CAS  Google Scholar 

  33. Melin AM, Carbonneau MA, Rebeyrotte N (1986) Fatty acids and carbohydrate-containing lipids in four Micrococcaceae strains. Biochimie 68:1201–1209

    Article  PubMed  CAS  Google Scholar 

  34. Bender GR, Sutton SV, Marquis RE (1986) Acid tolerance, proton permeabilities, and membrane ATPases of oral streptococci. Infect Immun 53:331–338

    PubMed  CAS  Google Scholar 

  35. Harper DS, Loesche WJ (1984) Growth and acid tolerance of human dental plaque bacteria. Arch Oral Biol 29:843–848

    Article  PubMed  CAS  Google Scholar 

  36. Costerton JW, Rozee KR, Cheng KJ (1983) Colonization of particulates, mucus, and intestinal tissue. Prog Food Nutr Sci 7:91–105

    PubMed  CAS  Google Scholar 

  37. Hazell SL, Lee A (1986) Campylobacter pyloridis, urease, hydrogen ion back diffusion, and gastric ulcers. Lancet II:15–17

    Article  Google Scholar 

  38. Luck JM, Seth TN (1923) Gastric urease. Biochem J 18:1227–1231

    Google Scholar 

  39. Fitzgerald O, Murphy P (1950) Studies on the physiological chemistry and clinical significance of urease and urea with special reference to the stomach. Ir J Med Sci 292:97–159

    Google Scholar 

  40. Kornberg HL, Davies RE (1955) Gastric urease. Physiol Rev 35:169–177

    PubMed  CAS  Google Scholar 

  41. Fleshier B, Gabuzda G (1965) Effect of ammonium chloride and urea infusions on ammonium levels and acidity of gastric juice. Gut 6:349–356

    Article  Google Scholar 

  42. Murakami M, Yoo JK, Inada M, Miyake T (1988) Effect of ammonia on the gastric mucosa in rats: pathophysiological importance of urease in gastric ulcer disease. Jpn J Pharmacol 47:330–332

    Article  PubMed  CAS  Google Scholar 

  43. Steer HW (1985) The gastro-duodenal epithelium in peptic ulceration. J Pathol 146:355–362

    Article  PubMed  CAS  Google Scholar 

  44. Wyatt JI, Rathbone BJ, Dixon MF, Heatley RV (1987) Campylobacter pylori and acid induced gastric metaplasia in the pathogenesis of duodenitis. J Clin Pathol 40:841–848

    Article  PubMed  CAS  Google Scholar 

  45. Caselli M, Bovolenta MR, Aleotti A, Trevisani L, Stabellini G, Ricci N (1988) Epithelial morphology of duodenal bulb and Campylobacter-like organisms. J Submicrosc Cytol 20:237–242

    CAS  Google Scholar 

  46. Carrick J, Lee A, Hazell S, Ralston M, Daskalopoulos G (1989) Campylobacter pylori, duodenal ulcer, and gastric metaplasia: possible role of functional heterotopic tissue in ulcerogenesis. Gut 30:790–797

    Article  PubMed  CAS  Google Scholar 

  47. Barer MR, Elliott TS, Berkeley D, Thomas JE, Eastham EJ (1988) Cytopathic effects of Campylobacter pylori urease. J Clin Pathol 41:597

    Article  PubMed  CAS  Google Scholar 

  48. Burns TR, Mace ML, Greenberg SD, Jachimczyk JA (1985) Ultrastructure of acute ammonia toxicity in the human lung. Am J Forensic Med Pathol 6:204–210

    Article  PubMed  CAS  Google Scholar 

  49. Gregorios JB, Mozes LW, Norenberg LO, Norenberg MD (1986) Effect of cyclic AMP on ammonia-induced alterations in primary astrocyte cultures. J Neuropathol Exp Neurol 45:396–407

    Article  PubMed  CAS  Google Scholar 

  50. Renau-Piqueras J, O’Connor JE, Baguena-Cervellera R, Grisolia S (1986) Ammonium chlorideinduced alterations in growth kinetics and ultrastructure of murine neuroblastoma cells. A flow cytometric and stereologic study. Virchows Arch [B] 50:271–283

    Article  CAS  Google Scholar 

  51. Klucinski W, Targowski SP (1984) Ammonia toxicity for mammalian and avian lymphocytes from blood. Immunopharmacology 8:47–52

    Article  PubMed  CAS  Google Scholar 

  52. Visek WJ (1984) Ammonia: its effects on biological systems, metabolic hormones, and reproduction. J Dairy Sci 67:481–498

    Article  PubMed  CAS  Google Scholar 

  53. Ölender EJ, Woods D, Kozol R, Fromm D (1986) Salicylate effects on proton gradient dissipation by isolated gastric mucosal surface cells. Proc Soc Exp Biol Med 183:177–185

    PubMed  Google Scholar 

  54. Perona R, Serrano R (1988) Increased pH and tumorigenicity of fibroblasts expressing a yeast proton pump. Nature 334:438–440

    Article  PubMed  CAS  Google Scholar 

  55. de Groot AP, Feron VJ, Immel HR (1988) Induction of hyperplasia in the bladder epithelium of rats by a dietary excess of acid or base: implications for toxicity/carcinogenicity testing. Food Chem Toxicol 26:425–434

    Article  PubMed  Google Scholar 

  56. Smith CE (1984) Hyperplastic lesions of the primitive meninx of fathead minnows, Pimephales promelas, induced by ammonia: species potential for carcinogen testing. Natl Cancer Inst Monogr 65:119–125

    PubMed  CAS  Google Scholar 

  57. Rathbone BJ, West AP, Wyatt JI, Johnson AW, Tompkins DS (1986) Campylobacter pyloridis, urease, and gastric ulcers. Lancet II:400–401

    Article  Google Scholar 

  58. Fauchere JL, Rosenau A, Bonneville F (1989) Virulence factors of Campylobacter pylori. Gastroenterol Clin Biol 13:59B-64B

    PubMed  CAS  Google Scholar 

  59. Richardson SE, Karmali MA, Becker LE, Smith CR (1988) The histopathology of the hemolytic uremic syndrome associated with verocytotoxin producing Escherichia coli infections. Hum Pathol 19:1102–1108

    Article  PubMed  CAS  Google Scholar 

  60. Grisham MB, Jefferson MM, Melton DF, Thomas EL (1984) Chlorination of endogenous amines by isolated neutrophils. Ammonia-dependent bactericidal, cytotoxic, and cytolytic activities of the chloramines. J Biol Chem 259:10404–10413

    PubMed  CAS  Google Scholar 

  61. Wilson CB, Weaver WM (1985) Comparative susceptibility of group B streptococci and Staphylococcus aureus to killing by oxygen metabolites. J Infect Dis 152:323–329

    Article  PubMed  CAS  Google Scholar 

  62. Beaman BL, Black CM, Doughty F, Beaman L (1985) Role of superoxide dismutase and catalase as determinants of pathogenicity of Nocardia asteroides: importance in resistance to microbicidal activity of human polymorphonuclear neutrophils. Infect Immun 47:135–141

    PubMed  CAS  Google Scholar 

  63. Schwartz CE, Krall J, Norton L, McKay K, Kay D, Lynch RE (1983) Catalase and superoxide dismutase in Escherichia coli. J Biol Chem 258:6277–6281

    PubMed  CAS  Google Scholar 

  64. Hazell SL, Markesich DC, Evans DJ, Evans DG, Graham DY (1989) Influence of media supplements on the growth and survival of Campylobacter pylori. Eur J Clin Microbiol Infect Dis 8:597–602

    Article  PubMed  CAS  Google Scholar 

  65. Hochman A, Shemesh A (1987) Purification and characterization of a catalase-peroxidase from the photosynthetic bacterium Rhodopseudomonas capsulata. J Biol Chem 262:6871–6876

    PubMed  CAS  Google Scholar 

  66. Meir E, Yagil E (1985) Further characterization of the two catalases in Escherichia coli. Curr Microbiol 12:315–320

    Article  CAS  Google Scholar 

  67. Goldberg I, Hochman A (1989) Three different types of catalase in Klebsiella pneumoniae. Arch Biochem Biophys 268:124–128

    Article  PubMed  CAS  Google Scholar 

  68. Wayne LG, Diaz GA (1982) Serological, taxonomic, and kinetic studies of the T and M classes of mycobacterial catalase. Int J Syst Bacteriol 32:296–304

    Article  CAS  Google Scholar 

  69. Smith GW, Tasman-Jones C, Wiggins PM, Lee SP (1985) Pig gastric mucus: a one-way barrier for H+. Gastroenterology 89:1313–1318

    PubMed  CAS  Google Scholar 

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Authors
  1. S. L. Hazell
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Editors and Affiliations

  1. Medizinische Klinik II, Krankenanstalten der Stadt Remscheid, Akademisches Lehrkrankenhaus der Universität Bonn, Hans-Potyka-Str. 28, 5630, Remscheid, Germany

    H. Menge

  2. Abteilung für Innere Medizin mit Schwerpunkt Gastroenterologie, Klinikum Steglitz der Freien Universität Berlin, Hindenburgdamm 30, 1000, Berlin 45, Germany

    M. Gregor

  3. Department of Gastroentero-Hepatology, Academisch Ziekenhuis bij de Universiteit van Amsterdam, Academisch Medisch Centrum, Meibergdreef 9, 1105 AZ, Amsterdam Zuidoost, The Netherlands

    G. N. J. Tytgat

  4. Division of Gastroenterology, Department of Internal Medicine, University of Virginia, Charlottesville, Virginia, 22908, USA

    B. J. Marshall

  5. Public Health Laboratory, Gloucestershire Royal Hospital, Great Western Road, Gloucestershire, GL1 3NN, UK

    C. A. M. McNulty

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© 1991 Springer-Verlag Berlin Heidelberg

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Hazell, S.L. (1991). Urease and Catalase as Virulence Factors of Helicobacter pylori . In: Menge, H., Gregor, M., Tytgat, G.N.J., Marshall, B.J., McNulty, C.A.M. (eds) Helicobacter pylori 1990. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75726-6_1

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  • DOI: https://doi.org/10.1007/978-3-642-75726-6_1

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