Detection and Typing of Genital Papillomaviruses: Nucleic Acid Hybridization and Type-Specific Antigen
Human papillomaviruses (HPV) are classified on the basis of the relatedness of their nucleic acids (Coggin and zur Hausen 1979). Different hybridization techniques allow the discrimination of 23 genital HPV types, of which HPV 6, 11, 16, and 18 are most prevalent (zur Hausen and Schneider 1987). Serologic tests had been of no value for the taxonomic discrimination of genital HPVs since the genital lesions produce only a limited amount of viral antigen with a concentration too low for detection by immunization tests. Now that the complete DNA sequences of several genital HPV types are known, type-specific viral proteins have recently become available. The viral antigens are synthesized in bacterial expression vectors, and antisera are produced by immunization of animals (Seedorf et al. 1987).
KeywordsHuman Papilloma Virus Cervical Intraepithelial Neoplasia Southern Blot Hybridization Sandwich Hybridization Nucleic Acid Strand
Unable to display preview. Download preview PDF.
- Coggin R, zur Hausen H (1979) Workshop on papillomaviruses and cancer. Cancer Res 39: 545–546Google Scholar
- Cox KH, de Leon DV, Angerer LM, Angerer RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101: 485–502Google Scholar
- Gissmann L, Forbes B, Pawlita M, Schneider A (1986) Filter in situ hybridization–a sensitive method to detect papillomavirus DNA single cell. In: Lerman LS (ed) DNA probes, applications in genetic and infectious disease and cancer. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 157–162Google Scholar
- Gissmann L, Kirchhoff T, von Knebel-Döberitz C, Jochmus-Kudielka I, Meinhardt G, Schneider A (1989) Analysis of repeated cervical swabs from Pap-negative women for the presence of HPV 16 DNA. J Cell Biochem [Suppl] 13C: 171Google Scholar
- Jenison SA, Firzlaff JM,_Langenberg A, Galloway DA (1988) Identification of immunoreactive antigens of human papillomavirus type 6b by using Escherichia con-expressed fusion proteins. J Virol 62: 2115–2123Google Scholar
- Jochmus-Kudielka I, Schneider A, Braun R, Kimmig R, Koldovsky U, Schneweis KE, Seedorf K, Gissmann L (1989) Antibodies against the human papillomavirus type 16 early proteins in human sera: correlation of anti-E7 reactivity with cervical cancer. J Natl Cancer Inst 81: 1698–1704PubMedCrossRefGoogle Scholar
- Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
- Martinez J, Smith R, Framer M, Resau J, Alger L, Daniel R, Gupta J, Shah K, Naghashfar Z (1988) High prevalence of genital tract papillomavirus infection in female adolescents. Paediatrics 82: 604–608Google Scholar
- Pratili MA, LeDoussal V, Harvey P, Laval C, Bertrand F, Jibard N, Croissant O, Orth G (1986) Human papillomaviruses in the epithelial cells of the cervix uteri: frequency of types 16 and 18. Preliminary results of a clinical, cytologic and viral study. J Gynecol Obstet Biol Reprod (Paris) 15: 45–50Google Scholar
- Tornita Y, Shirasawa H, Simizu B (1987b) Expression of human papillomavirus types 6b and 16 L1 open reading frames in Escherichia coli: detection of a 56,000-Dalton polypeptide containing genus-specific (common) antigens. J Virol 61: 2389–2394Google Scholar
- Walboomers JM, Melchers WJ, Mullink H, Meijer CJ, Struyk A, Quint WG, van der Noordaa J, ter Schegget J (1988) Sensitivity of in situ detection with biotinylated probes of human papilloma virus type 16 DNA in frozen tissue sections of squamous cell carcinomas of the cervix. Am J Pathol 131: 587–594PubMedGoogle Scholar
- Zur Hausen H, Schneider A (1987) The role of papillomaviruses in human ano-genital cancer. In: Howley P, Salzmann NP (eds) The Papovaviridae, the papillomaviruses. Plenum, New York, pp 245–263Google Scholar