Detection and Typing of Genital Papillomaviruses: Nucleic Acid Hybridization and Type-Specific Antigen
Human papillomaviruses (HPV) are classified on the basis of the relatedness of their nucleic acids (Coggin and zur Hausen 1979). Different hybridization techniques allow the discrimination of 23 genital HPV types, of which HPV 6, 11, 16, and 18 are most prevalent (zur Hausen and Schneider 1987). Serologic tests had been of no value for the taxonomic discrimination of genital HPVs since the genital lesions produce only a limited amount of viral antigen with a concentration too low for detection by immunization tests. Now that the complete DNA sequences of several genital HPV types are known, type-specific viral proteins have recently become available. The viral antigens are synthesized in bacterial expression vectors, and antisera are produced by immunization of animals (Seedorf et al. 1987).
KeywordsFormaldehyde Phenol Manifold Electrophoresis Anemia
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