Advertisement

Detection and Typing of Genital Papillomaviruses: Nucleic Acid Hybridization and Type-Specific Antigen

  • A. Schneider
  • G. Schlunck
Conference paper

Abstract

Human papillomaviruses (HPV) are classified on the basis of the relatedness of their nucleic acids (Coggin and zur Hausen 1979). Different hybridization techniques allow the discrimination of 23 genital HPV types, of which HPV 6, 11, 16, and 18 are most prevalent (zur Hausen and Schneider 1987). Serologic tests had been of no value for the taxonomic discrimination of genital HPVs since the genital lesions produce only a limited amount of viral antigen with a concentration too low for detection by immunization tests. Now that the complete DNA sequences of several genital HPV types are known, type-specific viral proteins have recently become available. The viral antigens are synthesized in bacterial expression vectors, and antisera are produced by immunization of animals (Seedorf et al. 1987).

Keywords

Human Papilloma Virus Cervical Intraepithelial Neoplasia Southern Blot Hybridization Sandwich Hybridization Nucleic Acid Strand 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Banks L, Spence P, Androphy E, Hubbert N, Matlashewski G, Murray A, Crawford L (1987a) Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas. J Gen Virol 68: 1351–1359PubMedCrossRefGoogle Scholar
  2. Banks L, Matlashewski G, Pim D, Churcher M, Roberts C, Crawford L (1987b) Expression of human papillomavirus type 6 and type 16 capsid proteins in bacteria and their antigenic characterization. J Gen Virol 68: 3081–3089PubMedCrossRefGoogle Scholar
  3. Beckmann AM, Meyerson D, Daling JR, Kiviat NB, Fenoglio CM, McDougall JK (1985) Detection and localisation of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes. J Med Virol 16: 265–273PubMedCrossRefGoogle Scholar
  4. Bernard HU, Oltersdorf T, Seedorf K (1987) Expression of the human papillomavirus type 18 E7 gene by a cassette-vector system for the transcription and translation of open reading frames in eukaryotic cells. EMBO J 6: 133–138PubMedGoogle Scholar
  5. Brandsma J, Burk R, Lancaster W, Pfister H, Schiffman MH (1989) Inter-laboratory variation as an explanation for varying prevalence estimates of human papillomavirus infection. Int J Cancer 43: 260–262PubMedCrossRefGoogle Scholar
  6. Brown DR, Chin MT, Strike DG (1988) Identification of human papillomavirus type 11 E4 gene products in human tissue implants from athymic mice. Virology 165: 262–267PubMedCrossRefGoogle Scholar
  7. Browne HM, Churcher MJ, Stanley MA, Smith GL, Minson AC (1988) Analysis of the L1 gene product of human papillomavirus type 16 by expression in a vaccinia virus recombinant. J Gen Virol 69: 1263–1273PubMedCrossRefGoogle Scholar
  8. Burk RD, Kadish AS, Calderin S, Romney SL (1986) Human papillomavirus infection of the cervix detected by cervicovaginal lavage and molecular hybridization: correlation with biopsy results and Papanicolaou smear. Am J Obstet Gynecol 154: 982–989PubMedGoogle Scholar
  9. Burns J, Graham AK, Frank C, Fleming KA, Evans MF, McGee JOD (1987) Detection of low-copy human papilloma virus DNA and m-RNA in routine paraffin sections of cervix by non-isotopic in situ hybridization. J Clin Pathol 40: 858–864PubMedCrossRefGoogle Scholar
  10. Coggin R, zur Hausen H (1979) Workshop on papillomaviruses and cancer. Cancer Res 39: 545–546Google Scholar
  11. Cox KH, de Leon DV, Angerer LM, Angerer RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101: 485–502Google Scholar
  12. Crum CP, Nadai N, Levine RU, Silverstein S (1986) In situ hybridization analysis of HPV 16 DNA sequences in early cervical neoplasia. Am J Pathol 123: 174–182PubMedGoogle Scholar
  13. Crum CP, Nuovo G, Friedman D, Silverstein SJ (1988) A comparison of biotin and isotope-labelled ribonucleic acid probes for in situ detection of HPV-16 ribonucleic acid in genital precancers. Lab Invest 58: 354–359PubMedGoogle Scholar
  14. De Villiers E-M, Wagner D, Schneider A, Wesch H, Miklaw H, Wahrendorf J, Papendick U, zur Hausen H (1987) Human papillomavirus infections in women without and with abnormal cervical cytology. Lancet 1: 703–705CrossRefGoogle Scholar
  15. Firzlaff JM, Kiviat NB, Beckmann AM, Jenison SA, Galloway DA (1988) Detection of human papillomavirus capsid antigens in various squamous epithelial lesions using antibodies directed against the L1 and L2 open reading frames. Virology 164: 467–477PubMedCrossRefGoogle Scholar
  16. Galloway DA, Jenison SA (1990) Characterization of the humoral immune response to genital papillomaviruses. Mol Biol Med 7: 59–72PubMedGoogle Scholar
  17. Gissmann L, Schwarz E (1985) Cloning of papillomavirus DNA. In: Becker Y (ed) Recombinant DNA research and virus. Nijhoff, Boston, pp 173–197CrossRefGoogle Scholar
  18. Gissmann L, Forbes B, Pawlita M, Schneider A (1986) Filter in situ hybridization–a sensitive method to detect papillomavirus DNA single cell. In: Lerman LS (ed) DNA probes, applications in genetic and infectious disease and cancer. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 157–162Google Scholar
  19. Gissmann L, Kirchhoff T, von Knebel-Döberitz C, Jochmus-Kudielka I, Meinhardt G, Schneider A (1989) Analysis of repeated cervical swabs from Pap-negative women for the presence of HPV 16 DNA. J Cell Biochem [Suppl] 13C: 171Google Scholar
  20. Jenison SA, Firzlaff JM,_Langenberg A, Galloway DA (1988) Identification of immunoreactive antigens of human papillomavirus type 6b by using Escherichia con-expressed fusion proteins. J Virol 62: 2115–2123Google Scholar
  21. Jochmus-Kudielka I, Schneider A, Braun R, Kimmig R, Koldovsky U, Schneweis KE, Seedorf K, Gissmann L (1989) Antibodies against the human papillomavirus type 16 early proteins in human sera: correlation of anti-E7 reactivity with cervical cancer. J Natl Cancer Inst 81: 1698–1704PubMedCrossRefGoogle Scholar
  22. Kafatos FC, Jones CW, Estratiadis A (1979) Determination of nucleic acid sequence homologies and relative concentration by a dot blot hybridization procedure. Nucleic Acids Res 7: 1541–1552PubMedCrossRefGoogle Scholar
  23. Landegren U, Kaiser R, Caskey CT, Hood L (1988) DNA diagnostics–molecular techniques and automation. Science 242: 229–237PubMedCrossRefGoogle Scholar
  24. Li CH, Shah KV, Seth A, Gilden RV (1987) Identification of the human papillomavirus 6b L1 open reading frame protein in condylomas and corresponding antibodies in human sera. J Virol 61: 2684–2690PubMedGoogle Scholar
  25. Li CH, Gilden RV, Showalter SD, Shah K (1988) Identification of the human papillomavirus E2 protein in genital tract tissues. J Virol 62: 606–609PubMedGoogle Scholar
  26. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
  27. Martinez J, Smith R, Framer M, Resau J, Alger L, Daniel R, Gupta J, Shah K, Naghashfar Z (1988) High prevalence of genital tract papillomavirus infection in female adolescents. Paediatrics 82: 604–608Google Scholar
  28. McCance DJ, Campion MJ, Clarkson PK, Chesters PM, Jenkins D, Singer A (1985) Prevalence of human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and invasive carcinoma of the cervix. Br J Obstet Gynaecol 92: 1101–1105PubMedCrossRefGoogle Scholar
  29. McConaughy BR, Laird CD, McCarthy BJ (1969) Nucleic acid reassociation in formamide. Biochemistry 8: 3289–3295PubMedCrossRefGoogle Scholar
  30. Mifflin TE, Bruns DE, Savory J (1988) Detection of papillomavirus DNA. Clin Chem 34: 1359PubMedGoogle Scholar
  31. Nagai N, Nuovo G, Friedman D, Crum CP (1987) Detection of papillomavirus nucleic acids in genital precancers with the in situ hybridization technique. Int J Gynecol Pathol 6: 366–379PubMedCrossRefGoogle Scholar
  32. Oltersdorf T, Seedorf K, Röwekamp W, Gissmann L (1987) Identification of human papillomavirus type 16 E7 protein by monoclonal antibodies. J Gen Virol 68: 2933–2938PubMedCrossRefGoogle Scholar
  33. Parkkinen S (1988) Nucleic acid sandwich hybridization in detection of HPV 16 DNA: technique and its clinical application. J Virol Methods 19: 69–77PubMedCrossRefGoogle Scholar
  34. Parkkinen S, Mäntyjärvi R, Syrjänen K, Ranki M (1986) Detection of human papillomavirus DNA by the nucleic acid sandwich hybridization method from cervical scraping. J Med Virol 20: 279–288PubMedCrossRefGoogle Scholar
  35. Pratili MA, LeDoussal V, Harvey P, Laval C, Bertrand F, Jibard N, Croissant O, Orth G (1986) Human papillomaviruses in the epithelial cells of the cervix uteri: frequency of types 16 and 18. Preliminary results of a clinical, cytologic and viral study. J Gynecol Obstet Biol Reprod (Paris) 15: 45–50Google Scholar
  36. Rigby PJW, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–252PubMedCrossRefGoogle Scholar
  37. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354PubMedCrossRefGoogle Scholar
  38. Schneider A, Oltersdorf T, Schneider V, Gissmann L (1987a) Distribution pattern of human papillomavirus 16 genome in cervical neoplasia by molecular in situ hybridization of tissue sections. Int J Cancer 39: 717–721PubMedCrossRefGoogle Scholar
  39. Schneider A, Hotz M, Gissmann L (1987b) Prevalence of genital HPV-infections in pregnant women. Int J Cancer 40: 198–201PubMedCrossRefGoogle Scholar
  40. Seedorf K, Oltersdorf T, Krämmer G, Röwekamp W (1987) Identification of early proteins of the human papilloma viruses type 16 (HPV 16) and type 18 (HPV 18) in cervical carcinoma cells. EMBO J 6: 139–144PubMedGoogle Scholar
  41. Shibata DK, Arnheim N, Martin WJ (1988) Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. J Exp Med 167: 225–230PubMedCrossRefGoogle Scholar
  42. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517PubMedCrossRefGoogle Scholar
  43. Stoler M, Broker TR (1986) In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma. Hum Pathol 17: 1250–1258PubMedCrossRefGoogle Scholar
  44. Syrjänen S, Partanen P, Mäntyjärvi R, Syrjänen K (1988) Sensitivity of in situ hybridization techniques using biotin-and 35 S-labelled human papilloma virus ( HPV) DNA probes. J Virol Methods 19: 225–238PubMedCrossRefGoogle Scholar
  45. Thomas PS (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201–5205PubMedCrossRefGoogle Scholar
  46. Tomita Y, Shirasawa H, Sekine H, Simizu B (1987a) Expression of the human papillomavirus type 6b L2 open reading frame in Escherichia coli: L2-ß-galactosidase fusion proteins and their antigenic properties. Virology 158: 8–14PubMedCrossRefGoogle Scholar
  47. Tornita Y, Shirasawa H, Simizu B (1987b) Expression of human papillomavirus types 6b and 16 L1 open reading frames in Escherichia coli: detection of a 56,000-Dalton polypeptide containing genus-specific (common) antigens. J Virol 61: 2389–2394Google Scholar
  48. Wagner D, Ikenberg H, Boehm N, Gissmann L (1984) Identification of human papillomavirus in cervical swabs by deoxyribonucleic acid in situ hybridization. Obstet Gynecol 64: 767–772PubMedGoogle Scholar
  49. Walboomers JM, Melchers WJ, Mullink H, Meijer CJ, Struyk A, Quint WG, van der Noordaa J, ter Schegget J (1988) Sensitivity of in situ detection with biotinylated probes of human papilloma virus type 16 DNA in frozen tissue sections of squamous cell carcinomas of the cervix. Am J Pathol 131: 587–594PubMedGoogle Scholar
  50. Wickenden C, Steele A, Malcolm AD, Coleman DV (1985) Screening for wart virus infection in normal and abnormal cervices by DNA hybridization of cervical scrapes. Lancet 1: 65–67PubMedCrossRefGoogle Scholar
  51. Wickenden C, Malcolm AD, Byrne M, Smith C, Anderson MC, Coleman DV (1987) Prevalence of HPV DNA and viral copy numbers in cervical scrapes from women with normal and abnormal cervices. J Pathol 153: 127–135PubMedCrossRefGoogle Scholar
  52. Zur Hausen H, Schneider A (1987) The role of papillomaviruses in human ano-genital cancer. In: Howley P, Salzmann NP (eds) The Papovaviridae, the papillomaviruses. Plenum, New York, pp 245–263Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. Schneider
  • G. Schlunck

There are no affiliations available

Personalised recommendations