Stimulation of Granulocyte Macrophage Progenitors via Monocyte/Macrophage Activation: A Fundamental Regulatory Pathway of Terminal Differentiation

  • E. Wunder

Abstract

The basic reason for testing a graft is to assure in advance its capacity to completely restore hematopoiesis, including early recovery and long-term production of mature cells of all lineages. Colonies growing in agar or methylcellulose after external stimulation indicate the most advanced, committed progenitors, which in vivo produce blood cells in the early recovery phase. Less differentiated precursor and multipotential cells capable of producing long-term recovery currently cannot be detected by simple assay systems in routine testing; probably they occur less frequently and must attach to feeder cells for self-renewal and production of advanced progenitors (see previous chapter). The feeder layer may represent an equivalent to their natural microenvironment in bone marrow, which provides not only attachment sites but also all necessary growth and differentiation factors.

Keywords

Agar Leukemia Anemia Assure Melphalan 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sovalat H, Liang H, Wunder E, Henon PR (1992) Flow cytometry characterization of CD34+ cells in bone marrow, cytapheresis products and cord blood at birth. Int J Cell Clon 10 [Suppl.1]: 20–22CrossRefGoogle Scholar
  2. 2.
    Wunder E, Sowala H, Lepers M, Henon PR (1990) The role of monocytes/macrophages in blood stem cell maturation; studies with highly purified precursor (CD34+) cells. B Marr Transpl 5 [Suppl. 1]: 11–12Google Scholar
  3. 3.
    Wunder E, Thing-Mortensen B, Sovalat H, Henon PR, Charbord P (1993) Role of mononuclear macrophages in the regulation of haemopoiesis: in vitro experiments and observations in vivo. In: van Furth R (ed) Hemopoietic growth factors and mononuclear phagocytes. Karger, BaselGoogle Scholar
  4. 4.
    Wunder E, Thing-Mortensen B, Schilling F, Henon PR (1993) Anomalous plasma concentrations and impaired secretion of growth factors in Fanconi’s anemia. Stem Cells 11 [Suppl. 2]: 144–149PubMedCrossRefGoogle Scholar
  5. 5.
    Wunder E, Sovalat H, Liang H, Eisenmann JC, Henon PR (1992) Mobilization of peripheral stem cells after high dose chemotherapy in multiple myeloma. Int J Cell Clon 10 [Suppl.1]: 38–40CrossRefGoogle Scholar
  6. 6.
    Wunder E, Baerenzung M, Mortensen BT (1992) Monocyte activation in hematopoietic stress. Int J Cell Clon 10 [Suppl. 1]: 12–15CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • E. Wunder
    • 1
  1. 1.Centre Hospitalier de MulhouseInstitut de Recherche en Hématologie et TransfusionMulhouseFrance

Personalised recommendations