Ex-Vivo Treatment of Graft by Long-Term Culture

  • J. Chang
  • T. M. Dexter


To establish human long-term bone marrow cultures (LTBMC), 2 X 107 nucleated bone marrow cells are suspended in 10 ml of Iscove’s medium containing 10% (vol: vol) fetal calf serum, 10% (vol: vol) horse serum and 5x101M hydrocortisone and added to flat bottomed plastic flasks which have been gassed with a mixture of 5% C02 in air. The cultures are maintained at 33 °C and at weekly intervals half the medium is removed and replaced with fresh medium. The cells in the overlying medium contain differentiated haematopoietic cells, mainly macrophages, neutrophils and progenitor cells, which can be assayed in soft gels for their ability to form colonies. Haematopoiesis and the production of progenitor cells in LTBMC can be maintained for 8 weeks or more [6].


Acute Myeloid Leukaemia Haematopoietic Stem Cell Leukaemic Cell Haematopoietic Cell Acute Myeloid Leukaemia Patient 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • J. Chang
    • 1
  • T. M. Dexter
    • 1
  1. 1.Department of Experimental HaematologyPaterson Institute, Christie HospitalManchesterUK

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