Cryopreservation and Infusion of Peripheral Blood Stem Cells
Peripheral blood stem cells (PBSC) may be obtained by apheresis, processed to reduce the volume and the mature blood cell contamination, cryopreserved, and then thawed for autologous infusion [1–3]. Eligible patients include those whose marrow is likely to be contaminated with malignant cells or who have received prior radiation therapy involving the iliac crests traditionally used for marrow harvest [4–6]. This paper describes the methods for processing, cryopreservation, storage, and infusion of thawed PBSC at the University of Nebraska Medical Center.
KeywordsPeripheral Blood Stem Cell Concentrate Product Freezing Medium Nebraska Medical Hematopoietic Reconstitution
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