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Cryopreservation and Infusion of Peripheral Blood Stem Cells

  • P. I. Warkentin
  • J. D. Jackson
  • A. Kessinger

Abstract

Peripheral blood stem cells (PBSC) may be obtained by apheresis, processed to reduce the volume and the mature blood cell contamination, cryopreserved, and then thawed for autologous infusion [1–3]. Eligible patients include those whose marrow is likely to be contaminated with malignant cells or who have received prior radiation therapy involving the iliac crests traditionally used for marrow harvest [4–6]. This paper describes the methods for processing, cryopreservation, storage, and infusion of thawed PBSC at the University of Nebraska Medical Center.

Keywords

Peripheral Blood Stem Cell Concentrate Product Freezing Medium Nebraska Medical Hematopoietic Reconstitution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • P. I. Warkentin
    • 1
    • 2
  • J. D. Jackson
    • 1
  • A. Kessinger
    • 3
  1. 1.Department of Pathology/MicrobiologyUniversity of Nebraska Medical CenterOmahaUSA
  2. 2.Department of PediatricsUniversity of Nebraska Medical CenterOmahaUSA
  3. 3.Department of Internal MedicineUniversity of Nebraska Medical CenterOmahaUSA

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