Skip to main content
SpringerLink
Log in

Ex Vivo and In Vivo Cytokine Studies in Irradiated Animals

  • Chapter
Peripheral Blood Stem Cell Autografts

  • 70 Accesses

Abstract

With the recent availability of a number of cloned colony-stimulating factors (CSF), preclinical studies of recombinant CSF in irradiated animals have been possible. These studies have been and will continue to be the prelude for clinical trials. This review will highlight previous studies which led to the first clinical trials and will discuss preclinical strategies for future clinical testing.

This work was supported in part by U.S. Public Health Service Grants T32-CA09445, P01-CA21737, R01-CA31618, R01-CA36725, K08-AM1509, N01-AI-85002, RFP N01-AI-85002, and R23-AII9254 awarded by the National Cancer Institute, DHHS, and the Minnesota Medical Foundation and Childres’s Cancer Research Fund. Center for Experimental Transplantation and Cancer Research publication # 64.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Donahue RE, Want EA, Stone DK, Kamen R, Wong GG, Sehgal PK, Nathan DG, Clark SC (1986) Stimulation of haematopoiesis in primates by continuous infusion of recombinant human GM-CSF. Nature 321: 872

    Article  PubMed  CAS  Google Scholar 

  2. Nienhuis AW, Donahue RE, Karisson S, Clark SC, Agricola B, Antinoff N, Pierce JE, Turner P, Anderson WF, Nathan DG (1987) Recombinant human granulocyte-macrophage colony-stimulating factor ( GM-CSF) shortens the period of neutropenia after autologous bone marrow transplantation in a primate model. J Clin Invest 80: 573

    Article  PubMed  CAS  Google Scholar 

  3. Monroy RL, Skelly RR, Mac Vittie TJ, Davis TA, Sauber JJ, Clark SC, Donahue RE (1987) The effect of recombinant GM-CSF on the recovery of monkeys transplanted with autologous bone marrow. Blood 70: 1696

    PubMed  CAS  Google Scholar 

  4. Gillio AP, Bonilla MA, Potter GK, Gabrilove JL, O’Reilly RJ, Souza LM, Welte K (1987) Effects of recombinant human granulocyte-colony stimulating factor on hematopoietic reconstitution after autologous bone marrow transplantation in primates. Transplant Proc 19: 153

    PubMed  CAS  Google Scholar 

  5. Vallera DA, Blazar BR (1989) T cell depletion for graft-versus-host-disease (GVHD) prophylaxis: a perspective on engraftment in mice and humans. Transplantation 47: 751

    Article  PubMed  CAS  Google Scholar 

  6. Blazar BR, Widmer MB, Soderling CCB, Gillis S, Vallera DA (1988) Enhanced survival but reduced engraftment in murine recipients of recombinant granulocyte/macrophage colony-stimulating factor follwing transplantation of T-cell-depleted histoincompatible bone marrow. Blood 72: 1148

    PubMed  CAS  Google Scholar 

  7. Blazar BR, Widmer MB, Cossman D, Sassenfeld HM, Vallera DA (1989) Improved survival and leukocyte reconstitution without detrimental effects on engraftment in murine recipients of human recombinant granulocyte colony-stimulating factor following transplantation of T-cell depleted histoincompatible bone marrow. Blood 74: 2264

    PubMed  CAS  Google Scholar 

  8. Okano A, Suzuki C, Takatsuki F, Akiyama Y, Loike K, Nakahata T, Hirano T, Kishimoto T, Ozawa K, Asano S (1989) Effect of interleukin-6 on hematopoiesis in bone marrow- transplanted mice. Transplantation 47: 738

    Article  PubMed  CAS  Google Scholar 

  9. Oppenheim J J, Neta R, Tiberghien P, Gress R, Kenny J J, Longo DL (1989) Interleukin-1 enhances survival of lethally irradiated mice treated with allogeneic bone marrow cells. Blood 74: 2257

    PubMed  CAS  Google Scholar 

  10. Socinski MA, Cannistra SA, Elias A, Antman KH, Schnipper L, Griffin JD (1988) Granulocyte-macrophage colony stimulating factor expands the circulating haemopoietic progenitor cell compartment in man. Lancet 1: 1194

    Article  PubMed  CAS  Google Scholar 

  11. Monroy RL, Davis TA, Donahue RE, Macvittie TJ (1989) Collection of enriched peripheral blood stem cells from primates treated with IL-1 and IL-3 in vivo. Blood (Suppl 1 ) 74: 75a

    Google Scholar 

  12. Donahue RE, Seehra J, Metzger M, Lefebvre D, Rock B, Carbone S, Nathan DG, Garnick M, Sehgal PK, Laston D, La Vallie E, McCoy J, Schendel PF, Norton C, Turner K, Yang Y-C, Clark SC (1988) Human IL-3 and GM-CSF act syngergistically in stimulating hematopoiesis in primates. Science 241: 1820

    Article  PubMed  CAS  Google Scholar 

  13. Fabian I, Bleiberg I, Riklis I, Kletter Y (1987) Enhanced reconstitution of hematopoietic organs in irradiated mice, following their transplantation with bone marrow cells pretreated with recombinant interleukin 3. Exp Hematol 15: 1140

    PubMed  CAS  Google Scholar 

  14. Blazar BR, Widmer MB, Soderling CCB, Urdal DL, Gillis S, Robison LL, Vallera DA (1988) Augmentation of donor bone marrow engraftment in histoincompatible murine recipients by granulocyte/macrophage colony-stimulating factor. Blood 71: 320

    PubMed  CAS  Google Scholar 

  15. Vallera DA, Blazar BR (1988) Depressed leukocyte reconstitution and engraftment in murine recipients of T cell-depleted histoincompatible marrow pretreated with interleukin 3. Transplantation 46: 616

    Article  PubMed  CAS  Google Scholar 

  16. Bodine DM, Karlsson S, Nienhuis AW (1989) Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells. Proc Natl Acad Sei USA 86: 8897

    Article  CAS  Google Scholar 

  17. Moreb J, Zucali JR, Gross MA, Weiner RS (1989) Protective effects of IL-1 on human hematopoietic progenitor cells treated in vitro with 4-hydroperoxycyclophosphamide. J Immunol 142: 1937

    PubMed  CAS  Google Scholar 

  18. Moreb J, Neta R, Zucali JR, Ledney GD, Weiner RS (1989) In vitro incubation with IL-1 protects pluripotent stem cells responsible for engraftment from 4-hydroperoxycyclo-phosphamide (4-HC). Blood (Suppl 1 ) 74: 148a

    Google Scholar 

  19. Blazar BR, Kersey JH, McGlave PB, Vallera DA, Lasky LC, Haake RJ, Bostrom B, Weisdorf DR, Epstein C, Ramasy NKC (1989) In vivo administration of recombinant human granulocyte/macrophage colony-stimulating factor in acute lymphoblastic leuke¬mia patients receiving purged autografts. Blood 73: 849

    PubMed  CAS  Google Scholar 

  20. Neta R, Oppenheim JJ, Douches SD (1988) Interdependence of the radioprotective effects of human recombinant interleukin la, tumor necrosis factor a, granulocyte colony- stimulating factor, and murine recombinant granulocyte-macrophage colony-stimulating factor. J Immunol 140: 108

    PubMed  CAS  Google Scholar 

  21. Uckun FM, Souza L, Waddick KG, Wick M, Song CW (1990) In vivo radioprotective effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) in lethally irradiated mice. Blood 75: 638

    PubMed  CAS  Google Scholar 

  22. Schuening FG, Storg R, Goehle S, Graham TC, Appelbaum FR, Hackman R, Souza LM (1989) Effect of recombinant human granulocyte colony-stimulating factor on hemato-poiesis of normal dogs and on hematopoietic recovery after otherwise lethal total body irradiation. Blood 74: 1308

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, Division of Bone Marrow Transplantation, University of Minnesota, Minneapolis, MN, 55455, USA

    B. R. Blazar

  2. Department of Therapeutic Radiology, Section on Experimental Cancer Immunology, University of Minnesota, Minneapolis, MN, 55455, USA

    D. Vallera

Authors
  1. B. R. Blazar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Vallera
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Service d’Hématologie et Institut de Recherche en Hématologie, Transfusion Hospital du Hasenrain, 87 av. d’Altkirch, 68051, Mulhouse Cedex, France

    Eckart W. Wunder M.D. (Médecin attaché Prof. assoc., Head of Stem Cell Laboratory) & Philippe R. Henon M.D. (Chef de Service Prof. assoc., Director of Research) (Médecin attaché Prof. assoc., Head of Stem Cell Laboratory) &  (Chef de Service Prof. assoc., Director of Research)

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Blazar, B.R., Vallera, D. (1993). Ex Vivo and In Vivo Cytokine Studies in Irradiated Animals. In: Wunder, E.W., Henon, P.R. (eds) Peripheral Blood Stem Cell Autografts. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75717-4_10

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-75717-4_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75719-8

  • Online ISBN: 978-3-642-75717-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation