Abstract
Bacterial restriction endonucleases recognize specific, short (4–8 base pair) nucleotide sequences in DNA. They bind at or near these sequences, then cleave DNA to generate either blunt-end or cohesive-end double-strand breaks. The role of DNA double-strand breaks in chromosome damage has long been a matter of some controversy (see Bryant 1988). By introducing restriction endonucleases into cells and examining their cytogenetic effects, investigators have now been able to define a more precise role for DNA double-strand breaks in chromosome damage and repair. In this report we review current methods for introducing restriction enzymes into cells, and the effects of restriction enzymes on the induction of DNA double-strand breaks, chromosome aberrations, and sister chromatid exchange (SCE).
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© 1990 Springer-Verlag Berlin Heidelberg
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Morgan, W.F., Winegar, R.A. (1990). The Use of Restriction Endonucleases to Study the Mechanisms of Chromosome Damage. In: Obe, G., Natarajan, A.T. (eds) Chromosomal Aberrations. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75682-5_8
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DOI: https://doi.org/10.1007/978-3-642-75682-5_8
Publisher Name: Springer, Berlin, Heidelberg
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