The Enzymology of Poxvirus DNA Replication
- 61 Downloads
DNA replication is arguably the most fundamental of biological processes and serves as a pivotal element in the poxvirus life cycle. Replication generates progeny genomes and initiates the transition to late gene expression. Unraveling the mechanism of poxvirus replication and identifying the panoply of enzymatic activities which it requires has been a focus of much recent research. The intent of this chapter is to move beyond previous reviews (McFadden and Dales 1982; Holowczak 1983) and focus on viral proteins whose participation in DNA replication is presumed on the basis of genetic or biochemical data. Implicit in this discussion was the choice of a broad definition of DNA replication, one which centers on DNA synthesis but also encompasses regulation of the topology of genomic DNA and its assembly into a nucleoprotein complex.
KeywordsVaccinia Virus Thymidine Kinase Ribonucleotide Reductase Nonpermissive Temperature Late Gene Expression
Unable to display preview. Download preview PDF.
- Bernad A, Zaballos A, Salas M, Blanco L (1987) Structural and functional relationships between prokaryotic and eukaryotic DNA polymerases. EMBOJ 6: 4219–4225Google Scholar
- Fogelsong PD, Bauer WR (1984) Effects of ATP and inhibitory factors on the activity of vaccinia virus type I topoisomerase. J Virol 49: 1–8Google Scholar
- Hanggi M, Bannwarth W, Stunnenberg HG (1986) Conserved TAAAT motif in vaccinia virus late promoters: overlapping TATA box and site of transcription initiation EMBOJ 5: 1071–1076Google Scholar
- Holowczak JA (1983) Poxvirus DNA replication. In: Becker Y (ed) Replication of viral and cellular genomes. Martinus Nijhoff Boston pp 205–236Google Scholar
- Larder BA, Kemp SD, Darby G (1987) Related functional domains in virus DNA polymerases. EMBOJ 6: 169–175Google Scholar
- McFadden G, Dales S (1982) Organization and replication of poxvirus DNA. In: Kaplan AS (ed) Organization and replication of viral DNA. CRC Boca Raton pp 173–190Google Scholar
- Moss B, Cooper N (1982) Genetic evidence for vaccinia virus encoded DNA polymerase: isolation of phosphonoacetic acid resistant enzyme from the cytoplasm of cells infected with mutant virus. J Virol 673–678Google Scholar
- Niles EG, Condit RC, Caro P, Davidson K, Matusick L, Seto J (1986) Nucleotide sequence and genetic map of the 16kb vaccinia virus HindIII D fragment. J Virol 153: 96–112Google Scholar
- Rosemond-Hornbeak H, Moss B (1973) Phosphoprotein components of vaccinia virus. J Virol 11: 961–970Google Scholar
- Sridhar P, Condit RC (1983) Selection for ts mutations in specific vaccinia virus genes: isolation of a phosphonoacetic acid-resistant, temperature sensitive virus mutant. J Virol 128: 444–457Google Scholar
- Wong SW, Wahl AF, Yuan PM, Arai N, Pearson BE, Arai K-I, Korn D, Hunkapiller MW, Wang TS-F (1988) Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases. EMBOJ 7: 37–47Google Scholar