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Effect of Human Recombinant Interleukin-3 in Long-term Bone Marrow Culture

  • R. Haas
  • D. Schwarz
  • S. Hohaus
  • E. Ogniben
  • G. Wulf
  • W. Hunstein
Conference paper

Abstract

Proliferation and differentiation of human stem cells depend on an appropriate microenvironment in the bone marrow where hemopoietic growth factors function as humoral messengers within a heterogeneous network of accessory cells. Various culture systems have been established for the evaluation of hemopoietic stem cells and accessory cells [1]. By the development of an adherent stromal cell layer, the human long-term bone marrow culture (LTBMC) for hemopoietic progenitor cells [2] supports self-renewal of the myeloid progenitor population. The stromal monolayer is formed by adipocytes, endothelial cells, macrophages, bone marrow fibroblasts, and blanket cells [3]. The extracellular matrix, which is essentially composed of fibronectin, laminin, collagen, and glycosaminoglycans, is able to absorb and compartmentalize the soluble hemopoietic growth factors within the stromal microenvironment [4]. Interleukin-3 (IL-3) is a growth factor involved in the regulation of hemopoiesis, not only stimulating myeloid, erythroid, mega-karyocyte, and lymphoid but also multipotential hemopoietic stem cells [5, 6]. The purpose of our study was to investigate the effect of recombinant human IL-3 (rhuIL-3) in human LTBMC with respect to its proliferative and differentiative capacity.

Keywords

Accessory Cell Nonadherent Cell Myeloid Progenitor Hemopoietic Progenitor Hemopoietic Progenitor Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag, Berlin Heidelberg 1990

Authors and Affiliations

  • R. Haas
  • D. Schwarz
  • S. Hohaus
  • E. Ogniben
  • G. Wulf
  • W. Hunstein

There are no affiliations available

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