Abstract
Proliferation of human cancer cell lines in vitro is stimulated by various polypeptide growth factors [1–3]. Sensitivity to polypeptide growth factors has also been reported for primary human tumors in vitro. At present, epidermal growth factor (EGF), insulin, and transferrin are most frequently used for in vitro experiments. However, published reports on the effects of these peptides on growth of clonogenic tumor cells from primary human specimens are controversial [4–6]. For their studies, most investigators have used a modified soft agar clonogenic assay using a Petri dish method as first published by Hamburger and Salmon [7]. The purpose of our present study was to determine the stimulatory effects of EGF, insulin, and transferrin using a recently developed capillary cloning system for solid human tumors [8–10]. To define the relative potency of these growth factors, we performed a head-to-head comparison and also determined the optimal concentrations for stimulation of clonogenic cancer cells.
This work was supported in part by a grant from the Verein zur Förderung der Tumorzentren in Niedersachsen and by Deutsche Forschungsgemeinschaft grant Ha 1347/2-1
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© 1990 Springer-Verlag, Berlin Heidelberg
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Depenbrock, H. et al. (1990). Growth Factor-Dependent Stimulation of Primary Human Tumor Cells. In: Freund, M., Link, H., Welte, K. (eds) Cytokines in Hemopoiesis, Oncology, and AIDS. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75510-1_24
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DOI: https://doi.org/10.1007/978-3-642-75510-1_24
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