VNTR Polymorphism: Reproducibility in techniques and interpretation
Variable number of tandem repeat (VNTR) markers, first described by Wyman and White (1980), are highly polymorphic DNA regions of great value for paternity and forensic investigations. The polymorphism is displayed by the relative migration of individual bands, alleles, according to the number of repeats. Some highly polymorphic VNTR systems include alleles so close to each other in size that they are difficult to distinguish in RFLP. In order to determine the technical reproducibility and the reliability of the interpretation of such systems duplicate DNA samples on parallel electrophoresis were analyzed with the probe pYNH24. The size of the fragments was calculated and the results compared.
KeywordsMigration Urea Electrophoresis
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