Advertisement

VNTR Polymorphism: Reproducibility in techniques and interpretation

  • G. Holmlund
  • B. Lindblom
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 3)

Abstract

Variable number of tandem repeat (VNTR) markers, first described by Wyman and White (1980), are highly polymorphic DNA regions of great value for paternity and forensic investigations. The polymorphism is displayed by the relative migration of individual bands, alleles, according to the number of repeats. Some highly polymorphic VNTR systems include alleles so close to each other in size that they are difficult to distinguish in RFLP. In order to determine the technical reproducibility and the reliability of the interpretation of such systems duplicate DNA samples on parallel electrophoresis were analyzed with the probe pYNH24. The size of the fragments was calculated and the results compared.

Keywords

Size Marker Relative Migration Forensic Investiga Urea Method Striction Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Lindblom B, Holmlund G (1988) Rapid DNA Purification for restriction fragment length polymorphism analysis. Gene Analysis Techniques 5: 97–101PubMedCrossRefGoogle Scholar
  2. Nakamura Y, Gillilan S, O’Connell P, Leppert M, Lathrop GM, Lalouel J-M and White R (1987) Isolation and mapping of a polymorphic DNA sequence pYNH24 on chromosome 2 (D2S44). Nucleic Acids Research 15: 10073PubMedCrossRefGoogle Scholar
  3. Wyman AR and White R (1980) A highly polymorphic locus in human DNA. Proceedings of the National Academy of Sciencies 77: 6754–6758CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • G. Holmlund
    • 1
  • B. Lindblom
    • 1
  1. 1.State Institute for Blood Group SerologyUniversity HospitalLinköpingSweden

Personalised recommendations