Identification of F13A*7 as a Population Specific Marker

  • S. A. Miller
  • D. D. Dykes
  • M. S. Schanfield
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 3)

Abstract

Factor XIII(F13), a precursor of fibrinoligase, is a tetramer with 2A and 2B subunits. F13B functions as a carrier and regulator of the enzymatically active F13A subunit. The two most common alleles are F13A*1 and F13A*2 with all other alleles appearing as rare or uncommon in most population groups reported in the 1iterature. (Board, 1979; Olaissen et al., 1983; Dykes et al., 1986). Ten different genetic variants of F13A have been reported using isoelectric focusing (IEF) techniques (Dykes et al, 1988). F13A can only be detectable in plasma samples, therefore it cannot be used in blood stain analysis. In parentage testing the exclusion probability of F13A=0.15. For this study we tested F13A on Whites and Blacks using IEF separation detected by immunoblotting. This report identifies the allele F13A*7 to be more specific for Black populations.

Keywords

Sucrose Agarose BCIP 

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References

  1. Board PG (1979) Genetic polymorphism of the A subunit of human coagulation Factor XIII. Am J Hum Genet 31: 116–124.PubMedGoogle Scholar
  2. Dykes DD, Miller SA, Polesky H (1986) Gene frequency of F13A and F13B in U.S. Whites, Blacks, Amerindians and Mexican-Americans. Advances in Forensic Haemogenetics 1, Springer-Verlag, Berlin Heidelberg.Google Scholar
  3. Dykes DD, Graham K, Johnson K, Miller S, Mount M, Schoener C, Polesky H (1988) Incidence of rare variants among serum proteins and RBC enzymes in U.S. Whites and Blacks. Advances in Forensic Haemogenetics 2. Springer-Ver1ag, Berlin Heidelberg.Google Scholar
  4. Olaissen B, Siverts A, Gedde-Dahl T Jr, Jonassen R, Teisberg P (1983) Coagulation factor 13A and B polymorphisms, referate-band des 10. Int Kongresses der Ges for Forensische B1utgruppen-kunde, München, p 459–465.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • S. A. Miller
    • 1
  • D. D. Dykes
    • 1
  • M. S. Schanfield
    • 1
  1. 1.Analytical Genetic Testing CenterDenverUSA

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