Isoelectric Focusing in Miniaturized Gels: Application to GC, PI, Tf and ORM Subtyping in Central Spain
Since the introduction of isoelectric focusing (IEF) for the analysis of protein polymorphisms of forensic science interest, several technical modifications have been developed to improve its reproducibility and resolution capacity. These include: IEF in a mixture of “separators” and carrier ampholytes (separator-IEF; SIEF) (Caspers et al. 1977; Gill et al. 1984); IEF in immobilized pH gradients (IEF-IPG) (Bjellquist et al. 1982; Cleve et al. 1982) and IEF in IPG-CA supplemented gels (hybrid-IEF; HIEF) (Atland et al. 1985).
KeywordsSucrose Phenol Urea Electrophoresis Polyacrylamide
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