Isoelectric Focusing in Miniaturized Gels: Application to GC, PI, Tf and ORM Subtyping in Central Spain

  • A. Alonso
  • G. Visedo
  • M. Sancho
  • J. Fernández-Piqueras
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 3)

Abstract

Since the introduction of isoelectric focusing (IEF) for the analysis of protein polymorphisms of forensic science interest, several technical modifications have been developed to improve its reproducibility and resolution capacity. These include: IEF in a mixture of “separators” and carrier ampholytes (separator-IEF; SIEF) (Caspers et al. 1977; Gill et al. 1984); IEF in immobilized pH gradients (IEF-IPG) (Bjellquist et al. 1982; Cleve et al. 1982) and IEF in IPG-CA supplemented gels (hybrid-IEF; HIEF) (Atland et al. 1985).

Keywords

Sucrose Phenol Urea Electrophoresis Polyacrylamide 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. Alonso
    • 1
  • G. Visedo
    • 2
  • M. Sancho
    • 1
  • J. Fernández-Piqueras
    • 2
  1. 1.ToxicologiaInstituto NacionalMadridSpain
  2. 2.Dpto. Biología, Facultad de Ciencias, C-15Universidad Autónoma de MadridMadridSpain

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