Rapid Preparation of DNA for RFLP Analysis and DNA Fingerprinting
The standard procedure for preparation of eucaryotic DNA includes several time consuming steps such as digestion of proteins with proteinase K and subsequent extractions of aminoacids and protein fragments with phenol (Maniatis 1982). The method described here is based on protein disintegration with SDS-urea (Meinke 1974) instead of proteinase K.
KeywordsSucrose Phenol Urea EDTA Bromide
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- Maniatis T (1982) In: Maniatis T, Fritsch EF and Sambrook J (eds) Molecular cloning, Cold Spring Harbor Laboratory, New York, pp 458–459Google Scholar