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Rapid Preparation of DNA for RFLP Analysis and DNA Fingerprinting

  • G. Holmlund
  • P. Hyltén
  • K. Friberg
  • B. Lindblom
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 3)

Abstract

The standard procedure for preparation of eucaryotic DNA includes several time consuming steps such as digestion of proteins with proteinase K and subsequent extractions of aminoacids and protein fragments with phenol (Maniatis 1982). The method described here is based on protein disintegration with SDS-urea (Meinke 1974) instead of proteinase K.

Keywords

Boric Acid Size Marker RFLP Analysis Protein Fragment Subsequent Extraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Lindblom B, Holmlund G (1988) Rapid DNA purification for restriction fragment length polymorphism analysis. Gene Analysis Techniques 5:97–101.PubMedCrossRefGoogle Scholar
  2. Maniatis T (1982) In: Maniatis T, Fritsch EF and Sambrook J (eds) Molecular cloning, Cold Spring Harbor Laboratory, New York, pp 458–459Google Scholar
  3. Meinke W, Goldstein DA and Hall MR (1974) Rapid isolation of mouse DNA from cells in tissue culture. Analythical Biochemistry 58: 82–88CrossRefGoogle Scholar
  4. Nakamura Y, Gillilan S, O’Connell P, Leppert M, Lathrop GM, Lalouel J-M and White R (1987) Isolation and mapping of a polymorphic DNA sequence NH24 on chromosome 2 (D2S44). Nucleic Acids Research 15: 10073PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • G. Holmlund
    • 1
  • P. Hyltén
    • 1
  • K. Friberg
    • 1
  • B. Lindblom
    • 1
  1. 1.State Institute for Blood Group SerologyUniversity HospitalLinköpingSweden

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