Analysis of PCR Products (pMCT118) by Polyacrylamide Gel Electrophoresis

  • B. Budowle
  • A. M. Giusti
  • R. C. Allen
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 3)


Identity tests rely on the genetic differences among individuals. The most informative genetic markers for the genetic characterization of people are variable number of tandem repeat (VNTR) loci (Nakamura, et al 1987). The detection of VNTRs is made possible by restriction fragment length polymorphism (RFLP) analysis via Southern Blotting (Southern, 1975). The RFLP procedure, although extremely effective for VNTR analyses, is time consuming and requires isotopic detection to type VNTRs in 10–50 ng of human genomic DNA samples (Budowle and Baechtel, 1989). Additionally, due to the inability of the technology to resolve discrete alleles of the VNTR loci that provide the greatest sensitivity of detection, more elaborate statistical analyses are required than for traditional genetic marker systems.


Restriction Fragment Length Polymorphism Restriction Fragment Length Polymorphism Analysis Restriction Fragment Length Polymorphism Typing Polymerase Chain Reaction Amplification Product Informative Genetic Marker 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • B. Budowle
    • 1
  • A. M. Giusti
    • 1
  • R. C. Allen
    • 2
  1. 1.Laboratory DivisionFSRTC, FBI AcademyQuanticoUSA
  2. 2.Department of PathologyMUSCCharlestonUSA

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