Analysis of PCR Products (pMCT118) by Polyacrylamide Gel Electrophoresis
Identity tests rely on the genetic differences among individuals. The most informative genetic markers for the genetic characterization of people are variable number of tandem repeat (VNTR) loci (Nakamura, et al 1987). The detection of VNTRs is made possible by restriction fragment length polymorphism (RFLP) analysis via Southern Blotting (Southern, 1975). The RFLP procedure, although extremely effective for VNTR analyses, is time consuming and requires isotopic detection to type VNTRs in 10–50 ng of human genomic DNA samples (Budowle and Baechtel, 1989). Additionally, due to the inability of the technology to resolve discrete alleles of the VNTR loci that provide the greatest sensitivity of detection, more elaborate statistical analyses are required than for traditional genetic marker systems.
KeywordsRestriction Fragment Length Polymorphism Restriction Fragment Length Polymorphism Analysis Restriction Fragment Length Polymorphism Typing Polymerase Chain Reaction Amplification Product Informative Genetic Marker
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- Budowle, B., Baechtel, F. S. (1989) Modification to improve the effectiveness of restriction fragment length polymorphism typing. Appl. Theor. Electrol (in press).Google Scholar
- Hochstrasser, D., Patchornik, A., Merril, C. (1988) Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining. Anal. Biochem. 173: 412–423.Google Scholar
- Horn, G. T., Richards, B., Klinger, K. W. (1989) Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. Nuc. Acids Res. 17: 2140.Google Scholar
- Kasai, K., Nakamura, Y., White. R. (1989) Amplification of VNTR locus by the polymerase chain reaction (PCR). Proceedings of an International Symposium on the Forensic Aspects of DNA Analysis, Governmental Printing Office, Washington, D. C. (in press).Google Scholar
- Nakamura, Y., Carlson, M, Krapcho, K., White, R. (1988) Isolation and mapping of a polymorphic DNA sequence (pMCT118) on Chromosome lp (D1S80). Nuc. Acids Res. 16: 9364.Google Scholar
- Southern E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517.Google Scholar