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Adult Ventricular Rat Heart Muscle Cells

  • H. M. Piper
  • A. Volz
  • P. Schwartz

Abstract

Isolated and cultured cardiomyocytes have facilitated a large variety of experimental approaches which either require cardiomyocyte material in great purity or direct experimental access to single cardiomyocytes. Techniques for the isolation of cardiomyocytes as well as those for primary cultures of such cells have been difficult to establish, due to a number of reasons including (a) In heart tissue the muscle cells are firmly connected to each other by intercalated discs [20] and the extracellular matrix network [57]; these connections are difficult to cleave without injuring the cells. (b) The cardiomyocyte is a large, polygonal, rigid cell that is more easily damaged by mechanical impact than are many other cell types. (c) The adult cardiomyocyte does not divide [40], thus the number of cells initially isolated cannot be increased in cultures [47].

Keywords

Fetal Calf Serum Bovine Serum Albumin Solution Intercalate Disc Adult Cardiomyocytes Rapid Attachment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • H. M. Piper
  • A. Volz
  • P. Schwartz

There are no affiliations available

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