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Microcarrier Cultures of Endothelial Cells

  • R. Spahr
  • H. M. Piper

Abstract

Microcarrier cultures of endothelial cells have a number of interesting applications. With the use of microcarriers the surface for growing anchorage-dependent cells can be enlarged in a given volume of culture medium. Therefore, microcarrier cultures are an ideal system to produce large quantities of cultured cells. Cells cultured on microcarriers can also be easily transferred from one experimental system to another without trypsinization, e.g., from two separate cultures of endothelial and smooth muscle cells into one defined coculture of both cell types [2]. Furthermore, packed columns of endothelial cells grown on microcarriers represent a useful model for studies on endothelial metabolism requiring a large amount of endothelial material. Such a column can be perfused in the same manner as an isolated organ.

Keywords

Polystyrene Bead Spinner Flask Newborn Calf Serum Microcarrier Culture Porcine Aortic Endothelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • R. Spahr
  • H. M. Piper

There are no affiliations available

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