Microcarrier Cultures of Endothelial Cells

  • R. Spahr
  • H. M. Piper


Microcarrier cultures of endothelial cells have a number of interesting applications. With the use of microcarriers the surface for growing anchorage-dependent cells can be enlarged in a given volume of culture medium. Therefore, microcarrier cultures are an ideal system to produce large quantities of cultured cells. Cells cultured on microcarriers can also be easily transferred from one experimental system to another without trypsinization, e.g., from two separate cultures of endothelial and smooth muscle cells into one defined coculture of both cell types [2]. Furthermore, packed columns of endothelial cells grown on microcarriers represent a useful model for studies on endothelial metabolism requiring a large amount of endothelial material. Such a column can be perfused in the same manner as an isolated organ.


Polystyrene Bead Spinner Flask Newborn Calf Serum Microcarrier Culture Porcine Aortic Endothelial Cell 
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© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • R. Spahr
  • H. M. Piper

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