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Bluetongue Viruses pp 21–41Cite as

Bluetongue Virus Structural Components

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 162))

Abstract

The structural components of bluetongue virus (BTV), the prototype of the orbivirus genus, has been the subject of a number of reviews (Verwoerd et al. 1979; Gorman and Taylor 1985; Spence et al. 1984). The main features can be summarized as follows: BTV is an icosahedral-shaped particle consisting of a segmented double-stranded RNA genome encapsidated in a double-layered protein coat. Removal of the outer protein layer activates a viral-associated RNA polymerase which transcribes the ten genome segments into 10 mRNAs which are in turn translated into at least seven structural and three nonstructural proteins. The characteristic features of these different structural components will be reviewed in this chapter. The key to the elucidation of the structural characteristics has been the ability to isolate and purify large amounts of BTV.

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References

  • Almeida JD, Bradburne AF, Wreghitt TG (1979) The effect of sodium thiocyanate on virus structure. J Med Virol 4: 269–277

    PubMed  CAS  Google Scholar 

  • Appleton JA, Letchworth GJ (1983) Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286–299

    PubMed  CAS  Google Scholar 

  • Bellamy AR, Shapiro RE, August JT, Joklik WK (1967) Studies on reovirus RNA. I. Characterization of reovirus genome RNA. J Mol Biol 29: 1–17

    PubMed  CAS  Google Scholar 

  • Borden EC, Shope RE, Murphy FA (1971) Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus—a new taxonomic group. Physicochemical and serological studies. J Gen Virol 13: 261–271

    PubMed  CAS  Google Scholar 

  • Boyle JF, Holmes KV (1986) RNA-binding proteins of bovine rotavirus. J Virol 58: 561–568

    PubMed  CAS  Google Scholar 

  • Bremer CW (1976) A gel electrophoretic study of the protein and nucleic acid components of African horsesickness virus. Onderstepoort J Vet Res 43: 193–200

    PubMed  CAS  Google Scholar 

  • Cowley JA, Gorman BM (1987) Genetic reassortants for identification of the genome segment coding for the bluetongue virus hemagglutinin. J Virol 61(7): 2304–2306

    PubMed  CAS  Google Scholar 

  • Cowley JA, Gorman BM (1989) Cross-neutralization of genetic reassortants of bluetongue virus serotypes 20 and 21. Vet Microbiol 19: 37–51

    PubMed  CAS  Google Scholar 

  • De Villiers E-M (1974) Comparison of the capsid polypeptides of various bluetongue virus serotypes. Intervirology 3: 47–53

    PubMed  Google Scholar 

  • Eaton BT, Hyatt AD (1989) Association of bluetongue virus with the cytoskeleton. Subcell Biochem 15:229–269

    Google Scholar 

  • Eaton BT, Hyatt AD, White JR (1987) Association of bluetongue virus with the cytoskeleton. Virology 157: 107–116

    PubMed  CAS  Google Scholar 

  • Eaton BT, Hyatt AD, White JR (1988) Localization of the nonstructural protein NS1 in bluetongue virus-infected cells and its presence in virus particles. Virology 163: 527–537

    PubMed  CAS  Google Scholar 

  • Els HJ (1973) Electron microscopy of bluetongue virus RNA. Onderstepoort J Vet Res 40: 73–76

    PubMed  CAS  Google Scholar 

  • Els HJ, Verwoerd DW (1969) Morphology of bluetongue virus. Virology 38: 213–219

    PubMed  CAS  Google Scholar 

  • Foster NM, Alders MA, Walton TE (1978) Continuity of the dsRNA genome of bluetongue virus. Curr Microbiol 1: 171–174

    CAS  Google Scholar 

  • Fukusho A, Ritter GD, Roy P (1987) Variation in the bluetongue virus neutralization protein VP2. J Gen Virol 68: 2967–2973

    PubMed  CAS  Google Scholar 

  • Ghiasi H, Purdy MA, Roy P (1985) The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17. Virus Res 3:181–190

    PubMed  CAS  Google Scholar 

  • Ghiasi H, Fukusho A, Eshita Y, Roy P (1987) Identification and characterization of conserved and variable regions in the neutralization VP2 gene of bluetongue virus. Virology 160: 100–109

    PubMed  CAS  Google Scholar 

  • Gorman BM, Taylor J (1985) Orbiviruses. In: Fields BN, Knipe DM, Chanock RM, Melnick, JL, Roizman B, Shope RE (eds) Virology, Raven Press New York, pp 907–925

    Google Scholar 

  • Gorman BM, Taylor J, Walker PJ, Davidson WL, Brown F (1981) Comparisons of bluetongue type 20 with certain viruses of the bluetongue and eubenangee serological groups of orbiviruses. J Gen Virol 57: 251–261

    PubMed  CAS  Google Scholar 

  • Gould AR (1987) The complete nucleotide sequence of bluetongue virus serotype 1 RNA 3 and a comparison with other geographic serotypes from Australia, South Africa and the United States of America, and with other orbivirus isolates. Virus Res 7: 169–183

    PubMed  CAS  Google Scholar 

  • Gould AR (1988a) Conserved and non-conserved regions of the outer coat protein, VP2, of the Australian bluetongue serotype 1 virus, revealed by sequences comparison to the VP2 of North American BTV serotype 10. Virus Res 9: 145–158

    CAS  Google Scholar 

  • Gould AR (1988b) Nucleotide sequence of the Australian bluetongue virus serotype 1 RNA segment 10. J Gen Virol 69: 945–949

    CAS  Google Scholar 

  • Gould AR (1988c) The use of recombinant DNA probes to group and type orbiviruses. A comparison of Australian and South African isolates. Arch Virol 99: 205–220

    CAS  Google Scholar 

  • Gould AR, Pritchard LI (1988) The complete nucleotide sequence of the outer coat protein, VP5, of the Australian bluetongue virus (BTV) serotype 1 reveals conserved and non-conserved sequences. Virus Res 9: 285–292

    PubMed  CAS  Google Scholar 

  • Gould AR, Hyatt AD, Eaton BT (1988) Morphogenesis of a bluetongue virus variant with an amino acid alteration at a neutralization site in the outer coat protein, VP2. Virology 165: 23–32

    PubMed  CAS  Google Scholar 

  • Grubman MJ, Appleton JA, Letchworth III GJ (1983) Identification of bluetongue virus type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity. Virology 131: 355–366

    PubMed  CAS  Google Scholar 

  • Gumm ID, Newman JFE (1982) The preparation of purified bluetongue virus group antigen for use as a diagnostic reagent. Arch Virol 72: 83–93

    PubMed  CAS  Google Scholar 

  • Hall SJ, Van Dijk AA, Huismans H (1989) Complete nucleotide sequence of gene segment 8 encoding non-structural protein NS2 of South African bluetongue virus serotype 10. Nucleic Acids Res 17: 457

    PubMed  CAS  Google Scholar 

  • Hübschle OJB (1980) Bluetongue virus hemagglutination and its inhibition by specific sera. Arch Virol 64: 133–140

    PubMed  Google Scholar 

  • Hübschle OJB, Yang C (1983) Purification of the group-specific antigen of bluetongue virus by chromatofocussing. J Virol Methods 6: 171–178

    PubMed  Google Scholar 

  • Huismans H (1979) Protein synthesis in bluetongue virus-infected cells. Virology 92: 385–396

    PubMed  CAS  Google Scholar 

  • Huismans H, Howell PG (1973) Molecular hybridization studies on the relationships between different serotypes of bluetongue virus and on the difference between the virulent and attenuated strains of the same serotype. Onderstepoort J Vet Res 40: 93–104

    PubMed  CAS  Google Scholar 

  • Huismans H, Verwoerd DW (1973) Control of transcription during the expression of the bluetongue virus genome. Virology 52: 81–88

    PubMed  CAS  Google Scholar 

  • Huismans H, Joklik WK (1976) Reovirus-coded polypeptides in infected cells: Isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA respectively. Virology 70:411–424

    PubMed  CAS  Google Scholar 

  • Huismans H, Els HJ (1979) Characterization of the tubules associated with the replication of three different orbiviruses. Virology 92: 397–406

    PubMed  CAS  Google Scholar 

  • Huismans H, Bremer CW (1981) A comparison of an Australian bluetongue virus isolate (CSIRO 19) with other bluetongue virus serotypes by cross-hybridization and cross-immune precipitation. Onderstepoort J Vet Res 48: 59–67

    PubMed  CAS  Google Scholar 

  • Huismans H, Erasmus BJ (1981) Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort J Vet Res 48: 51–58

    PubMed  CAS  Google Scholar 

  • Huismans H, Cloete M (1987) A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specified RNA. Virology 158: 373–380

    PubMed  CAS  Google Scholar 

  • Huismans H, Bremer CW, Barber TL (1979) The nucleic acid and proteins of epizootic haemorrhagic disease virus. Onderstepoort J Vet Res 46: 95–104

    PubMed  CAS  Google Scholar 

  • Huismans H, Van der Walt NT, Cloete M, Erasmus BJ (1983) The biochemical and immunological characterization of bluetongue virus outer capsid polypeptides. In: Compans RW, Bishop DHL (eds) Double-stranded RNA viruses. Elsevier New York, pp 165–172

    Google Scholar 

  • Huismans H, Cloete M, Le Roux A (1987a) The genetic relatedness of a number of individual cognate genes of viruses in the bluetongue and closely related serogroups. Virology 161: 421–428

    CAS  Google Scholar 

  • Huismans H, Van der Walt NT, Cloete M, Erasmus BJ (1987b) Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheep. Virology 157: 172–179

    CAS  Google Scholar 

  • Huismans H, Van Dijk AA, Bauskin AR (1987c) In vitro phosphorylation and purification of a nonstructural protein of bluetongue virus with affinity for single-stranded RNA. J Virol 61: 3589–3595

    CAS  Google Scholar 

  • Huismans H, Van Dijk AA, Els HJ (1987d) Uncoating of parental bluetongue virus to core and subcore particles in infected L cells. Virology 157: 180–188

    CAS  Google Scholar 

  • Hyatt AD, Eaton BT (1988) Ultrastructural distribution of the major capsid proteins within bluetongue virus and infected cells. J Gen Virol 69: 805–815

    PubMed  CAS  Google Scholar 

  • Imai M, Akatani K, Ikegami N, Furuichi Y (1983) Capped and conserved terminal structures in human rotavirus genome double-stranded RNA segments. J Virol 47: 125–136

    PubMed  CAS  Google Scholar 

  • Inamaru S, Roy P (1987) Production and characterization of the neutralization antigen VP2 of bluetongue virus serotype 10 using a baculovirus expression vector. Virology 157: 472–479

    Google Scholar 

  • Kahlon J, Sugiyama K, Roy P (1983) Molecular basis of bluetongue virus neutralization. J Virol 48: 627–632

    PubMed  CAS  Google Scholar 

  • Kapuler AM (1970) An extraordinary temperature dependance of the reovirus transcriptase. Biochemistry 9: 4453–4457

    PubMed  CAS  Google Scholar 

  • Kiuchi A, Rao CD, Roy P (1983) Analyses of bluetongue viral RNA sequences. In: Compans RW, Bishop DHL (eds) Double-stranded RNA viruses. Elsevier, New York, pp 55–64

    Google Scholar 

  • Kowalik TF, Li JKK (1987) The genetic relatedness of United States prototype bluetongue viruses by RNA/RNA hybridization. Virology 158: 276–284

    PubMed  CAS  Google Scholar 

  • Lecatsas G (1968) Electron microscopic study of the formation of bluetongue virus. Onderstepoort J Vet Res 35: 139–149

    PubMed  CAS  Google Scholar 

  • Lee JW, Roy P (1986) Nucleotide sequence of a cDNA clone of RNA segment 10 of bluetongue virus (serotype 10). J Gen Virol 67: 2833–2837

    PubMed  CAS  Google Scholar 

  • Lee J, Roy P (1987) Complete sequence of the NS1 gene (M6 RNA) of US bluetongue virus serotype 10. Nucleic Acids Res 15: 7207

    PubMed  CAS  Google Scholar 

  • Letchworth GJ, Appleton JA (1983) Heterogeneity of neutralization-related epitopes within a bluetongue virus serotype. Virology 124: 300–307

    PubMed  CAS  Google Scholar 

  • Martin SA, Zweerink HJ (1972) Isolation and characterization of two types of bluetongue virus particles. Virology 50: 495–506

    PubMed  CAS  Google Scholar 

  • Mecham JO, Dean VC, Jochim MM (1986) Correlation of serotype specificity and protein structure of the five U.S. serotypes of bluetongue virus. J Gen Virol 67: 2617–2624

    PubMed  CAS  Google Scholar 

  • Mertens PPC, Sangar DV (1985) Analysis of the terminal sequences of the genome segments of four orbiviruses. Virology 140: 55–67

    PubMed  CAS  Google Scholar 

  • Mertens PPC, Brown F, Sangar DV (1984) Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135: 207–217

    PubMed  CAS  Google Scholar 

  • Mertens PPC, Burroughs JN, Anderson J (1987a) Purification and properties of virus particles, infectious subviral particles, and cores of bluetongue virus serotypes 1 and 4. Virology 156: 375–386

    Google Scholar 

  • Mertens PPC, Pedley S, Cowley J, Burroughs JN (1987b) A comparison of six different bluetongue virus isolates by cross-hybridization of the dsRNA genome segments. Virology 161: 438–447

    CAS  Google Scholar 

  • Mertens PPC, Pedley S, Cowley J, Burroughs JN, Corteyn AH, Jeggo MH, Jennings DM, Gorman BM (1989) Analysis of the roles of bluetongue virus outer capsid protein VP2 and VP5, in determination of virus serotype. Virology 170: 561–565

    PubMed  CAS  Google Scholar 

  • Oberst RD, Stott JL, Blanchard-Channell M, Osburn BI (1987) Genetic reassortment of bluetongue virus serotype 11 strains in the bovine. Vet Microbiol 15: 11–18

    PubMed  CAS  Google Scholar 

  • Oellerman RA, Els HJ, Erasmus BJ (1970) Characterization of African horsesickness virus. Arch Ges Virusforsch 29: 163–174

    Google Scholar 

  • Palmer EL, Martin ML, Murphy FA (1977) Morphology and stability of infantile gastroenteritis virus: comparison with reovirus and bluetongue virus. J Gen Virol 35: 403–414

    Google Scholar 

  • Pedley S, Mohamed MEH, Mertens PPC (1988) Analysis of genome segments from six different isolates of bluetongue virus using RNA-RNA hybridisation: a generalised coding assignment for bluetongue viruses. Virus Res 10: 381–390

    PubMed  CAS  Google Scholar 

  • Purdy MA, Ritter GD, Roy P (1986) Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10. J Gen Virol 67: 957–962

    PubMed  CAS  Google Scholar 

  • Rao CD, Kiuchi A, Roy P (1983) Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus. J Virol 46: 378–383

    PubMed  CAS  Google Scholar 

  • Ritter GD, Roy P (1988) Genetic relationships of bluetongue virus serotypes isolated from different parts of the world. Virus Res 11: 33–47

    PubMed  CAS  Google Scholar 

  • Romantschuk M, Olkkonen VM, Bamfort DH (1988) The nucleocapsid of bacteriophage Φ6 penetrates the host cytoplasmic membrane. EMBO J 7: 1821–1829

    PubMed  CAS  Google Scholar 

  • Roy P (1989) Bluetongue virus genetics and genome structure. Virus Res 13: 179–206

    PubMed  CAS  Google Scholar 

  • Roy P, Ritter Jr, GD, Akashi H, Collison E, Inaba Y (1985) A genetic probe for identifying bluetongue virus infections in vivo and in vitro. J Gen Virol 66: 1613–1619

    PubMed  CAS  Google Scholar 

  • Roy P, Fukusho A, Ritter GD, Lyon D (1988) Evidence for genetic relationship between RNA and DNA viruses from the sequence homology of a putative polymerase gene of bluetongue virus with that of vaccinia virus: conservation of RNA polymerase genes from diverse species. Nucleic Acids Res 16: 11759–11767

    PubMed  CAS  Google Scholar 

  • Shatkin AJ (1976) Capping of eukaryotic mRNAs, Cell 9: 645–653

    PubMed  CAS  Google Scholar 

  • Shatkin AJ, Sipe JD (1968) RNA polymerase activity in purified reovirus. Proc Natl Acad Sci USA 61: 1462–1469

    PubMed  CAS  Google Scholar 

  • Shatkin AJ, Furuichi Y, LaFiandra AJ, Yamakawa M (1983) Initiation of mRNA synthesis and 5′-terminal modification of reovirus transcripts. In: Compans RW, Bishop DHL (eds) Double-stranded RNA viruses. Elsevier, New York, pp 43–54

    Google Scholar 

  • Skehel JJ, Joklik WK (1969) Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores. Virology 39: 822–831

    PubMed  CAS  Google Scholar 

  • Smith RE, Furuichi Y (1980) Gene mapping of cytoplasmic polyhedrosis virus of silkworm by the full-length mRNA prepared under optimized conditions of transcription in vitro. Virology 103: 279–290

    PubMed  CAS  Google Scholar 

  • Spence RP, Moore NF, Nuttall PA (1984) The biochemistry of orbiviruses. Arch Virol 82: 1–18

    PubMed  CAS  Google Scholar 

  • Squire KRE, Chuang RY, Obburn BI, Knudson DL, Doi RH (1983) Rapid methods for comparing the double-stranded RNA genome profiles of bluetongue virus. Vet Microbiol 8: 543–553

    PubMed  CAS  Google Scholar 

  • Squire KRE, Chuang RY, Dunn SJ, Dangler CA, Falbo MT, Chuang LF, Osburn BI (1986) Multiple bluetongue virus-cloned genetic probes: application to diagnostics and bluetongue virus genetic relationships. Am J Vet Res 47: 1785–1788

    PubMed  CAS  Google Scholar 

  • Stanley NF (1967) Reoviruses. Br Med Bull 23: 150–154

    PubMed  CAS  Google Scholar 

  • Tokuhisa S, Inaba Y, Miura Y, Sato K (1981) Salt-dependent hemagglutination with bluetongue virus. Arch Virol 70: 75–78

    PubMed  CAS  Google Scholar 

  • Tsai KS, Karstad L (1970) Epizootic hemorrhagic disease virus of deer: an electron microscopic study. Can J Microbiol 16: 427–432

    PubMed  CAS  Google Scholar 

  • Unger RE, Chuang RY, Chuang LF, Osburn BI, Doi RH (1988a) The cloning of full-length genome segments 2, 5, 6 and 8 of bluetongue virus (BTV) serotype 17 and studies of their genetic relatedness to United States BTV serotypes. Virology 167: 296–298

    CAS  Google Scholar 

  • Unger RE, Chuang RY, Chuang LF, Doi RH, Osburn BI (1988b) Comparison of dot-blot and Northern blot hybridizations in the determination of genetic relatedness of United States bluetongue virus serotypes. J Virol Methods 22: 273–282

    CAS  Google Scholar 

  • Urakawa T, Roy P (1988) Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10. J Virol 62: 3919–3927

    PubMed  CAS  Google Scholar 

  • Van der Walt NT (1980) A haemagglutination and haemagglutination inhibition test for bluetongue virus. Onderstepoort J Vet Res 47: 113–117

    PubMed  Google Scholar 

  • Van Dijk AA, Huismans H (1980) The in vitro activation and further characterization of the bluetongue virus-associated transcriptase. Virology 104: 347–356

    PubMed  Google Scholar 

  • Van Dijk AA, Huismans H (1982) The effect of temperature on the in vitro transcription reaction of bluetongue virus, epizootic haemorrhagic disease virus and African horsesickness virus. Onderstepoort J Vet Res 49: 227–232

    PubMed  Google Scholar 

  • Van Dijk AA, Huismans H (1987) The identification of factors capable of reversing the core-mediated inhibition of the bluetongue virus transcriptase. Onderstepoort J Vet Res 54: 629–633

    PubMed  Google Scholar 

  • Van Dijk AA, Huismans H (1988) In vitro transcription and translation of bluetongue virus mRNA. J Gen Virol 69: 573–581

    PubMed  Google Scholar 

  • Verwoerd DW (1969) Purification and characterization of bluetongue virus. Virology 38: 203–212

    PubMed  CAS  Google Scholar 

  • Verwoerd DW, Huismans H (1972) Studies on the in vitro and the in vivo transcription of the bluetongue virus genome. Onderstepoort J Vet Res 39: 185–192

    PubMed  CAS  Google Scholar 

  • Verwoerd DW, Els HJ, De Villiers E-M, Huismans H (1972) Structure of the bluetongue virus capsid. J Virol 10: 783–794

    PubMed  CAS  Google Scholar 

  • Verwoerd DW, Huismans H, Erasmus BJ (1979) Orbiviruses. In: Fraenkel-Conrat H, Wagner RR (eds) Comprehensive virology vol 14. Plenum, New York pp 285–345

    Google Scholar 

  • Wade-Evans AM, Pan ZQ, Mertens PPC (1988) In vitro expression analysis of a cDNA clone of genome segment 5 from bluetongue virus, serotype 1 from South Africa. Virus Res 11: 227–240

    PubMed  CAS  Google Scholar 

  • Waldvogel AS, Stott JL, Squire KRE, Osburn BI (1986) Strain-dependent virulence characteristics of bluetongue virus serotype 11. J Gen Virol 67: 765–769

    PubMed  CAS  Google Scholar 

  • Whistler T, Newman JFE (1986) Peptide mapping of the group-specific antigens from the Australian bluetongue virus (BTV-20) and serotypes from Southern Africa and North America. Vet Microbiol 11: 13–24

    PubMed  CAS  Google Scholar 

  • Wilson L, Mesa I (1973) The mechanism of colchicine. J Cell Biol 58: 709–719

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, University of Pretoria, Pretoria, 0002, South Africa

    H. Huismans

  2. Biochemistry Section, Veterinary Research Institute, Onderstepoort , 0110, South Africa

    A. A. Van Dijk

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  1. H. Huismans
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  2. A. A. Van Dijk
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Editors and Affiliations

  1. Dept. of Environmental Health Sciences, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, 35294, USA

    Polly Roy MSc, Ph.D.

  2. Agricultural Research Service, Arthropod-borne Animal Diseases Research Laboratory, United States Dept. of Agriculture, Laramie, WY82071-3965, USA

    Barry M. Gorman MSc, Ph.D.

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Huismans, H., Van Dijk, A.A. (1990). Bluetongue Virus Structural Components. In: Roy, P., Gorman, B.M. (eds) Bluetongue Viruses. Current Topics in Microbiology and Immunology, vol 162. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75247-6_2

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