Pgp-1 (Ly 24) As a Marker of Murine Memory T Lymphocytes
It is generally accepted that the primary immune response to an antigen involves the formation of long-lived, antigen-specific memory cells, in addition to effector cells. These memory cells are thought to be responsible for the heightened immune response that follows secondary exposure to an antigen. As the secondary antibody response is generally characterized by the production of increased levels of antibodies having higher avidity for antigen (Eisen and Siskind 1964), it has been proposed that immunologic memory at the B-cell level includes both an increased frequency of antigen-reactive lymphocytes and a greater mean avidity of their antigen receptors (Andersson 1970; Celada 1971; Davie and Paul 1972). In the case of T-cell responses, little is known about the formation of memory cells during immunization. There is evidence that the frequency of antigen-specific T lymphocytes may increase after immunization (MacDonald et al. 1980). In addition, indirect evidence has been provided showing that T cells in immunized animals bear higher avidity antigen receptors compared with normal animals (MacDonald et al. 1982; Marrack et al. 1983). However, detailed analysis of memory T cells has been hampered by the lack of suitable surface markers allowing identification and isolation of these cells. As discussed below, recent studies from our laboratory indicate that murine memory T cells can be identified phenotypically by the surface marker Pgp-1. A more comprehensive review of T-cell memory will be presented elsewhere (Cerottini and MacDonald 1989).
KeywordsMajor Histocompatibility Complex Limit Dilution Analysis Sperm Whale Myoglobin Cytolytic Capacity Fetal Thymocyte
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