Subsets of Rat CD4+ T Cells Defined by Their Differential Expression of Variants of the CD45 Antigen: Developmental Relationships and In Vitro and In Vivo Functions

  • F. Powrie
  • D. Mason
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 159)


Thymus-derived lymphocytes have been shown to mediate a wide range of immunological functions both as direct effectors in cell-mediated immunity and as helper or inducer cells for B cells, macrophages, bone marrow cells and other T cells. In addition, the lymphokines that they produce have been shown to act not only on leucocytes but also on non-bone marrow derived cells. This wide range of T-cell functions has only recently been fully appreciated, but early work using alloantisera in mice established that T cells with different functional specializations could be distinguished phenotypically on the basis of whether or not they expressed what came to be called the CD8 antigen (Cantor and Boyse 1975).


CD45 Molecule Mixed Lymphocyte Culture Thoracic Duct Lymph Lymphokine Production Secondary Antibody Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arthur RP, Mason D (1986) T cells that help B cell responses to soluble antigen are distinguishable from those producing interleukin 2 on mitogenic or allogeneic stimulation. J Exp Med 163: 774–786PubMedCrossRefGoogle Scholar
  2. Barclay AN, Jackson DI, Willis AC, Williams AF (1987) Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequence near the NH2- terminus. EMBO J 6: 1259–1264PubMedGoogle Scholar
  3. Bell EB, Sparshott SM, Drayson MT, Ford WL (1987) The stable and permanent expansion of functional T lymphocytes in athymic nude rats after a single injection of mature T cells. J Immunol 139:1379–1384PubMedGoogle Scholar
  4. Bellgrau D, Smilik D, Wilson DB (1981) Induced tolerance in F1 rats to anti-major histocompatibility complex receptors on parental T cells. J Exp Med 153: 1660–1665PubMedCrossRefGoogle Scholar
  5. Beverley PCL (1986/87) Human T cell subsets. Immunol Lett 14: 263–267CrossRefGoogle Scholar
  6. Boom WH, Liano D, Abbas AK (1988) Heterogeneity of helper/inducer T lymphocytes II. Effects of interleukin-4 and interleukin 2-producing T cell clones on resting B lymphocytes. J Exp Med 167:1350–1363PubMedCrossRefGoogle Scholar
  7. Cantor H, Boyse EA (1975) Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med 141: 1390–1399PubMedCrossRefGoogle Scholar
  8. Cherwinski H, Schumacher JH, Brown KD, Mossman TR (1987) Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Thl and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays and monoclonal antibodies. J Exp Med 166:1229–1244PubMedCrossRefGoogle Scholar
  9. Cobbold S, Hale G, Waldmann H (1987) Non lineage, LFA-1 family and leukocyte common antigens: new and previously defined clusters. In: McMichael AJ (ed) Leukocyte typing III. Oxford University Press, Oxford, pp 788–807Google Scholar
  10. Dalchau R, Kirkley J, Fabre JW (1980) Monoclonal antibody to a human leukocyte specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol 10: 737–744PubMedCrossRefGoogle Scholar
  11. Dallman MJ (1982) Studies on the cellular basis of skin allograft rejection in the rat. D Phil thesis, OxfordGoogle Scholar
  12. Fabre JW, Williams AF (1977) Quantitative serological analysis of a rabbit anti-rat lymphocyte serum and preliminary biochemical characterization of the major antigen recognised. Transplantation 23:349–359PubMedCrossRefGoogle Scholar
  13. Ford WL, Burr W, Simonsen M (1970) A lymph node weight assay for the graft-versus-host reactivity of rat lymphoid cells. Transplantation 10: 258–266PubMedCrossRefGoogle Scholar
  14. Harp JA, Davis BS, Ewald SJ (1984) Inhibition of T cell responses to alloantigens and polyclonal mitogens by Ly-5 antisera. J Immunol 133: 10–15PubMedGoogle Scholar
  15. Inaba K, Steinman RM (1984) Resting and sensitized T lymphocytes exhibit distinct stimulatory (antigen-presenting cell) requirements for growth and lymphokine release. J Exp Med 160: 1717–1735PubMedCrossRefGoogle Scholar
  16. Joling P, Tielen FJ, Vaessen LMB, Huijbregts JMA, Rozing J (1985) New markers on T cell subpopulations defined by monoclonal antibodies. Transplant Proc 17: 1857–1863Google Scholar
  17. Killar L, MacDonald G, West J, Woods A, Bottomly K (1987) Cloned, la-restricted T cells that do not produce interleukin 4 (IL4)/B cell stimulatory factor 1 (BSF-1) fail to help antigen-specific B cells. J Immunol 138: 1674–1679PubMedGoogle Scholar
  18. Lelchuk R, Playfair JHL (1985) Serum IL-2 inhibitor in mice. I. Increase during infection. Immunology 56: 113–118PubMedGoogle Scholar
  19. Mason DW, Simmonds SJ (1988) The autonomy of CD8 + T cell in vitro and in vivo. Immunology 65: 249–257PubMedGoogle Scholar
  20. Mason DW, Brideau RJ, McMaster WR, Webb M, White RAH, Williams AF (1980) Monoclonal antibodies that define T-lymphocyte subsets in the rat. In: Kennett RH, McKern TJ, Bechtol KB (eds) Monoclonal antibodies. Plenum, New York, pp 251–273Google Scholar
  21. Mason DW, Penhale WJ, Sedgwick JD (1987) Preparation of lymphocyte subpopulations. In: Klaus GGB (ed) Lymphocytes, a practical approach. IRL Press, Oxford, pp 35–54Google Scholar
  22. Metcalf D (1984) The hemopoietic colony stimulating factors. Elsevier, Amsterdam, p 33Google Scholar
  23. Morimoto C, Letvin NL, Distaso JA, Aldrich WR, Schlossman SF (1985) The isolation and characterization of the human suppressor inducer T cell subset. J Immunol 134: 1508–1515PubMedGoogle Scholar
  24. Nelson DS, Sneider C (1974) Effect of normal mouse serum on mouse lymphocyte transformation in vitro. Eur J Immunol 4: 79–86PubMedCrossRefGoogle Scholar
  25. Paterson DJ, Jefferies WA, Green JR, Brandon MR, Corthesy P, Puklavec M, Williams AF (1987) Antigens of activated rat T lymphocytes including a molecule of 50 000 M r detected only on CD4 positive T blasts. Mol Immunol 24: 1281–1290PubMedCrossRefGoogle Scholar
  26. Pingel JR, Thomas ML (1989) Evidence that the leukocyte-common antigen is required for antigen- induced T lymphocyte proliferation. Cell 58: 1055–1065PubMedCrossRefGoogle Scholar
  27. Ralph SJ, Thomas ML, Morton CC, Trowbridge IS (1987) Structural variants of human T200 glycoprotein (leukocyte-common antigen). EMBO J 6: 1251–1257PubMedGoogle Scholar
  28. Reinherz, EL, Schlossman SF (1980) The differentiation and function of human T lymphocytes. A review. Cell 19: 821–827PubMedCrossRefGoogle Scholar
  29. Saga Y, Tung JS, Shen FW, Boyse EA (1987) Alternative use of 5’ exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages. Proc Natl Acad Sci USA 84: 5364–5368PubMedCrossRefGoogle Scholar
  30. Smith KA (1984) Interleukin 2. Annu Rev Immunol 2: 319–333PubMedCrossRefGoogle Scholar
  31. Sparrow RL, McKenzie IFC (1983) A function for human T200 in natural killer cytolysis. Transplantation 36: 166–171PubMedCrossRefGoogle Scholar
  32. Spickett GP, Brandon MR, Mason DW, Williams AF, Woollett GR (1983) MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen. J Exp Med 158: 795–810PubMedCrossRefGoogle Scholar
  33. Standring R, McMaster WR, Sunderland CA, Williams AF (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens. Eur J Immunol 8: 832–839PubMedCrossRefGoogle Scholar
  34. Streuli M, Hall LR, Saga Y, Schlossman SF, Saito H (1987a) Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J Exp Med 166:1548–1566PubMedCrossRefGoogle Scholar
  35. Streuli M, Matsuyama T, Morimoto C, Schlossman SF, Saito H (1987b) Identification of the sequence required for expression of the 2H4 epitope on the human leukocyte common antigens. J Exp Med 166: 1567–1572PubMedCrossRefGoogle Scholar
  36. Streuli M, Morimoto C, Schrieber M, Schlossman SF, Saito H (1988) Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual leukocyte-common antigens. J Immunol 141: 3910–3914PubMedGoogle Scholar
  37. Takeuchi T, Rudd CE, Schlossman SF, Morimoto C (1987) Induction of suppression following autologous mixed lymphocyte reaction; role of a novel 2H4 antigen. Eur J Immunol 17: 97–103PubMedCrossRefGoogle Scholar
  38. Terry LA, Brown MH, Beverley PCL (1988) The monoclonal antibody UCHLI, recognises a 180 000 MW component of the human leukocyte-common antigen, CD45. Immunology 64: 331–336PubMedGoogle Scholar
  39. Thomas ML, Barclay AN, Gagnon J, Williams AF (1985) Evidence from cDNA clones that the rat leukocyte common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80 000 M r Cell 41: 83–93PubMedCrossRefGoogle Scholar
  40. Thomas ML, Reynolds PJ, Chain A, Ben-Neriah Y, Trowbridge IS (1987) B-cell variant of mouse T200 (Ly-5): evidence for alternative in RNA splicing. Proc Natl Acad Sci USA 84: 5360–5363PubMedCrossRefGoogle Scholar
  41. Tonks NK, Charbonneau H, Diltz CD, Fischer EH, Walsh KA (1988) Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase. Biochem 27: 8695–8701CrossRefGoogle Scholar
  42. Trowbridge IS (1978) Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. J Exp Med 148: 313–322PubMedCrossRefGoogle Scholar
  43. Trowbridge IS, Nilsen-Hamilton M, Hamilton RT, Bevan MJ (1977) Preliminary characterization of two thymus-dependent xenoantigens from mouse lymphocytes. Biochem J 163: 211–217PubMedGoogle Scholar
  44. Williams AF, Galfré G, Milstein C (1977) Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell 12: 663–673PubMedCrossRefGoogle Scholar
  45. Woollett GR, Barclay AN, Puklavec M, Williams AF (1985) Molecular and antigenic heterogeneity of the rat leucocyte-common antigen from thymocytes and T and B lymphocytes. Eur J Immunol 15: 168–173PubMedCrossRefGoogle Scholar
  46. Yakura H, Shen FW, Bourcet E, Boyse EA (1983) On the function of Ly-5 in the regulation of antigen- driven B cell differentiation. Comparison and contrast with Lyb-2. J Exp Med 157: 1077–1088PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • F. Powrie
    • 1
  • D. Mason
    • 1
  1. 1.Medical Research Council Cellular Immunology UnitSir William Dunn School of Pathology, University of OxfordOxfordUK

Personalised recommendations