The Use of DNA Precipitation Assay for Evaluating DSB Induced by High and Low LET Radiations: Comparison with Sedimentation Results
The assays used for measurements of DNA double strand breaks (dsb), such as neutral sucrose gradient or filter elution, need sophisticated and expensive equipments as well as a great deal of time. Recently, Olive (1988) has proposed a relatively simple method for detecting dsb-related damage: a DNA precipitation assay in non-denaturing conditions. The principle of this method is that the treatment of cells with sodium dodecyl sulphate and KC1 causes cell proteins to precipitate, trapping long pieces of DNA. If breaks are introduced by radiation, DNA fragments remain in the supernatant.