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The Use of DNA Precipitation Assay for Evaluating DSB Induced by High and Low LET Radiations: Comparison with Sedimentation Results

  • M. Belli
  • D. T. Goodhead
  • F. Ianzini
  • T. J. Jenner
  • G. Simone
  • M. A. Tabocchini
Part of the NATO ASI Series book series (volume 54)

Abstract

The assays used for measurements of DNA double strand breaks (dsb), such as neutral sucrose gradient or filter elution, need sophisticated and expensive equipments as well as a great deal of time. Recently, Olive (1988) has proposed a relatively simple method for detecting dsb-related damage: a DNA precipitation assay in non-denaturing conditions. The principle of this method is that the treatment of cells with sodium dodecyl sulphate and KC1 causes cell proteins to precipitate, trapping long pieces of DNA. If breaks are introduced by radiation, DNA fragments remain in the supernatant.

Keywords

Alpha Particle Sodium Dodecyl Sulphate Initial Yield Chinese Hamster Cell Energy Cyclotron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Olive P L (1988) DNA precipitation assay: A rapid and simple method for detecting DNA damage in mammalian cells. Env Mol Mut 11:487–495CrossRefGoogle Scholar
  2. Thacker J, Stretch A, Goodhead D T (1982) The mutagenicity of a particles from plutonium-238. Rad Res 92:343–352CrossRefGoogle Scholar
  3. Thacker J, Stretch A, Stephens M A (1979) Mutation and inactivation of cultured mammalian cells exposed to beams of accelerated heavy ions 11. Chinese hamster V79 cells. Int J Rad Biol 36:137–148CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • M. Belli
    • 2
  • D. T. Goodhead
    • 3
  • F. Ianzini
    • 2
  • T. J. Jenner
    • 3
  • G. Simone
    • 1
  • M. A. Tabocchini
    • 2
  1. 1.Istituto FRAE – CNRBolognaItaly
  2. 2.ISSRomeItaly
  3. 3.MRCChiltonUK

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