Skip to main content
SpringerLink
Log in

The Use of DNA Precipitation Assay for Evaluating DSB Induced by High and Low LET Radiations: Comparison with Sedimentation Results

  • Conference paper
Book cover The Early Effects of Radiation on DNA

Part of the book series: NATO ASI Series ((ASIH,volume 54))

Abstract

The assays used for measurements of DNA double strand breaks (dsb), such as neutral sucrose gradient or filter elution, need sophisticated and expensive equipments as well as a great deal of time. Recently, Olive (1988) has proposed a relatively simple method for detecting dsb-related damage: a DNA precipitation assay in non-denaturing conditions. The principle of this method is that the treatment of cells with sodium dodecyl sulphate and KC1 causes cell proteins to precipitate, trapping long pieces of DNA. If breaks are introduced by radiation, DNA fragments remain in the supernatant.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Olive P L (1988) DNA precipitation assay: A rapid and simple method for detecting DNA damage in mammalian cells. Env Mol Mut 11:487–495

    Article  CAS  Google Scholar 

  • Thacker J, Stretch A, Goodhead D T (1982) The mutagenicity of a particles from plutonium-238. Rad Res 92:343–352

    Article  CAS  Google Scholar 

  • Thacker J, Stretch A, Stephens M A (1979) Mutation and inactivation of cultured mammalian cells exposed to beams of accelerated heavy ions 11. Chinese hamster V79 cells. Int J Rad Biol 36:137–148

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto FRAE – CNR, Via de Castagnoli, 1, 40126, Bologna, Italy

    G. Simone

  2. ISS, Rome, Italy

    M. Belli, F. Ianzini & M. A. Tabocchini

  3. MRC, Chilton, UK

    D. T. Goodhead & T. J. Jenner

Authors
  1. M. Belli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. T. Goodhead
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Ianzini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. J. Jenner
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Simone
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. A. Tabocchini
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Medical Research Council, Radiobiology Unit, Chilton, Didcot, Oxon, OX11 ORD, UK

    E. M. Fielden  & P. O’Neill  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Belli, M., Goodhead, D.T., Ianzini, F., Jenner, T.J., Simone, G., Tabocchini, M.A. (1991). The Use of DNA Precipitation Assay for Evaluating DSB Induced by High and Low LET Radiations: Comparison with Sedimentation Results. In: Fielden, E.M., O’Neill, P. (eds) The Early Effects of Radiation on DNA. NATO ASI Series, vol 54. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75148-6_32

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-75148-6_32

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75150-9

  • Online ISBN: 978-3-642-75148-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation