Thermal Transitions in Cardiac Troponin and its Subunits

  • L. A. Morozova
  • V. L. Shnyrov
  • N. B. Gusev
  • E. A. Permyakov
Part of the NATO ASI Series book series (volume 56)

Abstract

Cardiac troponin (Tn) takes part in the Ca2+-regulation of the cardiac muscle contraction. It consists of three subunits : troponin C (TnC)-Ca2+-binding protein, troponin I (TnI)-inhibitor of actomyosin ATPase and troponin T (TnT)-tropomyosin binding protein. However, it is still far from clear which changes occur in the conformation of subunits upon their interaction with each other. The parameters of thermal transitions of proteins reflect the changes in their structure. In the present work we studied the thermal denaturation of Tn subunits and their binary and ternary complexes in the presence and absence of Ca2+ by means of intrinsic fluorescence and microcalorimetry.

Keywords

Enthalpy EDTA Tyrosine Electrophoresis CaCl 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Morozova L.A., Gusev N.B., Shnyrov V.L., Permyakov E.A. (1988) Investigation of the physicochemical properties of troponin I an T from cardiac and skeletal muscles by methods of protein fluorescence and calorimetry (in Russian), Biokhimiya 53: 531–540Google Scholar
  2. Perry S.V., Cole H.A. (1974) Phosphorylation of troponin and the effect of the interactions between the components of the complex. Biochem. J. 141: 733–743PubMedGoogle Scholar
  3. Tsukui R., Ebashi S. (1973) Cardiac troponin. J. Biochem. 7: 1119–1121Google Scholar
  4. Wilkinson J.M. (1974) The preparation and properties of the components of troponin B. Biochim. Biophys. Acta 359: 379–388.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • L. A. Morozova
    • 1
  • V. L. Shnyrov
    • 1
  • N. B. Gusev
    • 2
  • E. A. Permyakov
    • 1
  1. 1.Institute of BiophysicsUSSR Academy of SciencesPushchino, Moscow RegionUSSR
  2. 2.Moscow State UniversityMoscowUSSR

Personalised recommendations