Thermal Transitions in Cardiac Troponin and its Subunits
Cardiac troponin (Tn) takes part in the Ca2+-regulation of the cardiac muscle contraction. It consists of three subunits : troponin C (TnC)-Ca2+-binding protein, troponin I (TnI)-inhibitor of actomyosin ATPase and troponin T (TnT)-tropomyosin binding protein. However, it is still far from clear which changes occur in the conformation of subunits upon their interaction with each other. The parameters of thermal transitions of proteins reflect the changes in their structure. In the present work we studied the thermal denaturation of Tn subunits and their binary and ternary complexes in the presence and absence of Ca2+ by means of intrinsic fluorescence and microcalorimetry.
KeywordsThermal Denaturation Cardiac Troponin Thermal Transition Wavelength Shift Tryptophan Fluorescence
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