About a Controversy Concerning the Existence of a Mitogen-Responsive S6-Kinase (Late-Eluting from Deae-Sephacel)

  • O. H. W. Martini
  • A. Lawen
  • M. Burger
Conference paper
Part of the NATO ASI Series book series (volume 56)


Phosphorylase kinase and the first phosphorylation cascade were discovered by tackling intracellular signal flux from the distal end of a signalling pathway, i.e. by asking for cause, not effect. During the last decade a comparable approach has been successfully applied in studies of anabolic signal flux towards the ribosomal protein S6. The approach utilizes the fact that serum (Lastick et al., 1977), epidermal growth factor (EGF), insulin and other anabolic agents (Thomas et al., 1982) - and also certain transforming proteins, notably of the tyrosine kinase family, - elicit phosphorylation of several S6 serines.


Filtration Lymphoma EDTA Tyrosine Serine 


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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • O. H. W. Martini
    • 1
  • A. Lawen
  • M. Burger
  1. 1.Biochemisches InstitutUniversität zu KölnKölnGermany

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