About a Controversy Concerning the Existence of a Mitogen-Responsive S6-Kinase (Late-Eluting from Deae-Sephacel)
Phosphorylase kinase and the first phosphorylation cascade were discovered by tackling intracellular signal flux from the distal end of a signalling pathway, i.e. by asking for cause, not effect. During the last decade a comparable approach has been successfully applied in studies of anabolic signal flux towards the ribosomal protein S6. The approach utilizes the fact that serum (Lastick et al., 1977), epidermal growth factor (EGF), insulin and other anabolic agents (Thomas et al., 1982) - and also certain transforming proteins, notably of the tyrosine kinase family, - elicit phosphorylation of several S6 serines.
KeywordsFiltration Lymphoma EDTA Tyrosine Serine
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