A Sensitive Colorimetric Assay for Protein Phosphatase Activity

  • T. P. Geladopoulos
  • T. G. Sotiroudis
  • A. E. Evangelopoulos
Conference paper
Part of the NATO ASI Series book series (volume 56)

Abstract

Phosphorylation of proteins is a posttranslational modification of major significance in cellular regulation (Krebs & Beavo 1979). Protein kinases catalyze the addition to, and phosphatases the removal of phosphate groups from serine, threonine, tyrosine and other amino acid residues (Boyer & Krebs, 1986). We present here a simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (Baykov et al. 1988). The method is described in detail elsewhere (Geladopoulos et al. 1991).

Keywords

Hydrolysis Albumin Tyrosine Acidity Serine 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • T. P. Geladopoulos
    • 1
  • T. G. Sotiroudis
    • 1
  • A. E. Evangelopoulos
    • 1
  1. 1.Institute of Biological ResearchThe National Hellenic Research FoundationAthensGreece

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