A Sensitive Colorimetric Assay for Protein Phosphatase Activity
Phosphorylation of proteins is a posttranslational modification of major significance in cellular regulation (Krebs & Beavo 1979). Protein kinases catalyze the addition to, and phosphatases the removal of phosphate groups from serine, threonine, tyrosine and other amino acid residues (Boyer & Krebs, 1986). We present here a simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (Baykov et al. 1988). The method is described in detail elsewhere (Geladopoulos et al. 1991).
KeywordsHydrolysis Albumin Tyrosine Acidity Serine
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