A Sensitive Colorimetric Assay for Protein Phosphatase Activity
Phosphorylation of proteins is a posttranslational modification of major significance in cellular regulation (Krebs & Beavo 1979). Protein kinases catalyze the addition to, and phosphatases the removal of phosphate groups from serine, threonine, tyrosine and other amino acid residues (Boyer & Krebs, 1986). We present here a simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (Baykov et al. 1988). The method is described in detail elsewhere (Geladopoulos et al. 1991).
KeywordsMalachite Green Ammonium Molybdate Protein Phosphatase Activity Phosphate Determination Bovine Brain Extract
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- Boyer PD, Krebs EG (eds) (1986) The Enzymes, vol XVII, Academic Press Inc Buss JE, Stull JT (1983) Meth Enzymol 99: 7–14Google Scholar
- Buss JE, Stull JT (1983) Meth Enzymol 99:7–14Google Scholar
- Geladopoulos TP, Sotiroudis TG, & Evangelopoulos AE (1991) Anal Biochem (in press)Google Scholar
- Weller M (ed.) (1979) Protein Phosphorylation, Pion Limited, LondonGoogle Scholar