Advertisement

The mom Operon of Bacteriophage MU is Regulated by a Combination of Transcriptional and Translational Controls

  • F. G. Wulczyn
  • F. Schmidt
  • A. Seiler
  • R. Kahmann
Conference paper
Part of the NATO ASI Series book series (volume 49)

Abstract

The mom operon of bacteriophage Mu has proven to be an attractive model system for the study of prokaryotic gene regulation. Of particular interest is the integration of transcriptional and post-transcriptional controls in the regulation of mom gene expression. mom regulation has been recently reviewed (Kahmann and Hattman, 1987), in this chapter we will focus on progress made in the last few years. Transcriptional regulation will be briefly discussed, followed by a more comprehensive treatment of post-transcriptional control. New data on the mRNA sites involved in post-transcriptional regulation will be presented, and the overexpression and purification of the regulatory Com protein will be described.

Keywords

Fusion Plasmid Tris Hydrochloride GATC Site Phage Development Attractive Model System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Belasco J G, Higgins CF (1988) Mechanisms of mRNA decay in bacteria: a perspective. Gene 72: 15–23PubMedCrossRefGoogle Scholar
  2. Bolker M, Kahmann R (1989) The Escherichia coli protein OxyR discriminates between methylated and unmethylated states of the phage Mu mom promoter. EMBO 8: 2403–2410Google Scholar
  3. Bolker M, Wulczyn F G, and Kahmann R (1989) Role of bacteriophage Mu C protein in activation of the mom gene promoter. J Bacteriol 171: 2019–2027PubMedGoogle Scholar
  4. Brot N, Caldwell P, Weissbach H (1980) Autogenous control of the Escherichia coli ribosomal protein L10 synthesis in vitro. Proc Natl Acad Sci 77: 2592–2595PubMedCrossRefGoogle Scholar
  5. Cole J R, Nomura M (1986) Changes in the half-life of ribosomal protein messenger RNA caused by translational repression. J Mol Biol 188: 383–392PubMedCrossRefGoogle Scholar
  6. Fling S, Gregerson D (1986) Peptide and protein molecular weight determination using a high- molarity Tris buffer system without Urea. Anal Biochem 155: 83–88PubMedCrossRefGoogle Scholar
  7. Gorski K, Roch J-M, Prentki P, and Krisch H M (1985) The stability of bacteriophage T4 gene 32 mRNA: a 5’ leader sequence that can stabilize mRNA transcripts. Cell 43: 461–469PubMedCrossRefGoogle Scholar
  8. Gralla J, Steitz J, Crothers D (1974) Direct physical evidence for secondary structure in an isolated fragment of R17 bacteriophage mRNA. Nature 248: 204–208PubMedCrossRefGoogle Scholar
  9. Hattman S, Ives J, (1984) SI nuclesae mapping of the phage Mu mom promoter: a model for the regulation of mom expression. Gene 29: 185–198PubMedCrossRefGoogle Scholar
  10. Hattman S, Ives J, Wall L, and Marie S (1987) The bacteriophage Mu com gene appears to specify a translation factor required for mom gene expression. Gene 55: 345–351PubMedCrossRefGoogle Scholar
  11. Kahmann R (1983) Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu. Cold Spring Harbor Symp Quant Biol 47: 639–646PubMedGoogle Scholar
  12. Kahmann R and Hattman S (1987) Regulation and expression of the mom gene. In: Symonds N et al (eds) Phage Mu.Cold Spring Harbor, N.Y., Cold Spring Harbor Laboratory, pp 93–110Google Scholar
  13. Kahmann R, Seiler A, Wulczyn F G, and Pfaff E (1985) The mom gene of bacteriophage Mu: a unique regulatory scheme to control a lethal function. Gene 39: 61–70PubMedCrossRefGoogle Scholar
  14. Klippel A, Mertens G, Patchinsky T, Kahmann R (1988) The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9. EMBO J 7: 1229–1237PubMedGoogle Scholar
  15. Nilsson G, Belasco J G, Cohen S N, and von Gabain A (1987) Effect of premature termination of translation on mRNA stability depends on the site of release. Proc Natl Acad Sci USA 84: 4890–4894PubMedCrossRefGoogle Scholar
  16. Schauder B, Blocker H, Frank R, McCarthy J (1987) Inducible expression vectors incorporating the E. coli atpE translational initiation region. Gene 52: 279–283Google Scholar
  17. Seiler A, Blocker H, Frank R, and Kahmann R (1986) The mom gene of bacteriophage Mu: the mechanism of methylation-dependent expression. EMBO J 5: 2719–2728PubMedGoogle Scholar
  18. Toussaint A (1976) The DNA modification function of temperate phage Mu-1. Virology 70: 17–27PubMedCrossRefGoogle Scholar
  19. Toussaint A (1977) The modification function of bacteriophage Mu-1 requires both a bacterial and a phage function. J Virol 23: 825–826PubMedGoogle Scholar
  20. Wulczyn F G, and Kahmann R (1987) Post-transcriptional regulation of the bacteriophage Mu mom gene by the com gene product. Gene 51: 139–147PubMedCrossRefGoogle Scholar
  21. Wyckoff E, Sampson L, Hayden M, Parr R, Huang W M, and Casjens S (1986) Plasmid vectors useful in the study of translation initiation signals. Gene 43: 281–286PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • F. G. Wulczyn
    • 1
  • F. Schmidt
    • 1
  • A. Seiler
    • 1
    • 2
  • R. Kahmann
    • 1
  1. 1.Institut für Genbiologische Forschung Berlin GmbHGermany
  2. 2.Max Planck Institut für molekülare GenetikGermany

Personalised recommendations