Culture Conditions Affect Differently the Translation of Individual Escherichia Coli mRNAs

  • N. Jacques
  • M. Chevrier-Miller
  • J. Guillerez
  • M. Dreyfus
Conference paper
Part of the NATO ASI Series book series (volume 49)


Individual bacterial mRNAs are translated at very different rates (Ray and Pearson, 1975; McCarthy, et al., 1985). Extensive work during the last decade has revealed that these unequal performances largely stem from sequence differences in the region contacted by the ribosome during initiation (Ribosome Binding Site or RBS: see Steitz, 1969). Thus, the nature of the initiation codon, the length of the Shine-Dalgarno sequence (SD), and the spacing between them, all contribute to the translational efficiency (Gold, 1988). Additional RBS sequence elements, less characterized to date, are probably also important (McCarthy et al., 1985; Petersen et al., 1988). Finally, the extent of secondary structure around RBSs profoundly affects their efficiencies (Gold, 1988; de Smit and van Duin, 1990). However, the role of these different elements has not been investigated as a function of growth conditions. The concentration of ribosomes, factors, precursors, etc, vary according to the metabolic state of the cell (Bremer and Dennis, 1987), and in vivo such changes affect differently the translational yields from individual mRNAs (Gualerzi et al., 1988). Should this also occur in vivo, then the translational yields from individual mRNAs would be expected to change according to growth conditions.


Elongation Rate Hybrid Gene Ribosome Binding Site Fusidic Acid mRNA Secondary Structure 
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  1. Adhya S and Gottesman M, (1978) Control of transcription termination, Ann. Rev. Biochem. 47: 967–996PubMedCrossRefGoogle Scholar
  2. Bingham A., Fulford F, Murray P., Dreyfus M., and Busby S, (1988) Translation initiation in the Escherichia Coli galE gene, in Genetics of Translation, Tuite H.F., Picard M., and Bolotin-Fukuhara M., Eds, Springer-Verlag, Berlin, pp 307–316Google Scholar
  3. Bremer H and Dennis PP, (1987) Modulation of the chemical composition and other parameters of the cell by growth rate, in Escherichia Coli and Salmonella Typhimurium: Cellular and molecular biology, FC Neidhardt, et al. eds., ASM, Washington DC, pp 1527–1542Google Scholar
  4. Busby S and Dreyfus M, (1983) Segment-specific mutagenesis of the regulatory region of the galactose operon, Gene 21: 123–133CrossRefGoogle Scholar
  5. Cashel M and Rudd KE, (1987) The stringent response, in Escherichia Coli and Salmonella Typhimurium: Cellular and molecular biology, F.C. Neidhardt, et al. eds, ASM, Washington DC, pp 1410–1438Google Scholar
  6. Dreyfus M, (1988) What constitute the signal for the initiation of protein synthesis on E. Coli mRNAs ? J. Mol. Biol. 204: 79–94Google Scholar
  7. Gold L, (1988) Posttranscriptionnal regulation mechanisms in E. coli, Ann. Rev. Biochem. 57: 199–233PubMedCrossRefGoogle Scholar
  8. Gualerzi CO, Calogero RA, Canonaco RA, Brombach M and Pon CL, (1988) Selection of mRNA by ribosomes during procaryotic translational initiation, in Genetics of Translation, Tuite H.F., Picard M., and Bolotin-Fukuhara M,. Eds, Springer-Verlag, Berlin, pp 317–330Google Scholar
  9. Hall MN, Gabay J, Débarbouillé M and Schwartz M, (1982) A role for mRNA secondary structure in the control of translation initiation, Nature 295: 616–618PubMedCrossRefGoogle Scholar
  10. Jaurin B, Grundstrôm T, Edlund T, and Normark S, (1981) The E. coli 6-lactamase attenuator mediates growth rate-dependent regulation, Nature 290: 221–225PubMedCrossRefGoogle Scholar
  11. Jones WR, Barcak GJ, and Wolf RE, (1990) Altered growth-rate dependent regulation of 6-Phosphogluconate deshydrogenase (…), J. Bact 172: 1197–1205PubMedGoogle Scholar
  12. Kenneil DE, (1986) The instability of mRNAs in bacteria, in Maximizing gene expression,W. Reznikoff and L. Gold Eds., Butterworth, USA, pp 101–142Google Scholar
  13. Lindahl L, Archer RH, McCormick JR, Freedman LP and Zengel JM, (1989) Translational coupling of the two proximal genes in the S10 ribosomal protein operon, J. Bact. 171: 2639–2645PubMedGoogle Scholar
  14. Lovett PS, (1990) Translational attenuation as the regulator of inducible cat genes, J. Bact 172: 1–6PubMedGoogle Scholar
  15. McCarthy JEG, Schairer HU and Sebald W, (1985) Translational initiation frequency of atp genes from E. coli: identification of an intercistronic sequence that enhances translation, EMBO J., 4: 519–526PubMedGoogle Scholar
  16. Nomura M, Bedwell DM, Yamagishi M, Cole JR and Kolb JM, (1987) RNA polymerase and regulation of RNA synthesis in E. coli: RNA polymerase concentration, stringent control, and ribosome feedback regulation, in RNA polymerase and the regulation of transcription W. Reznikoff, et al. eds. Elsevier pp 137–149Google Scholar
  17. Petersen GB, Stockwell PA, and Hill DF, (1988) mRNA recognition in E. coli: a possible second site of interaction with 16S ribosomal RNA, EMBO J., 7:3957– 3962Google Scholar
  18. Raibaud O, Mock M, and Schwartz M, (1984) A technique for integrating any DNA fragment into the chromosome of E. coli, Gene 29: 231–241PubMedCrossRefGoogle Scholar
  19. Ray PN and Pearson ML, (1975) Functional inactivation of bacteriophage λ morphogenetic gene mRNA, Nature 253: 647–650PubMedCrossRefGoogle Scholar
  20. de Smit MH and van Duin J, (1990) Control of procaryotic translational initiation by mRNA secondary structure, Prog. Nucl. Acid Res. and Mol. Biol. 38: in pressGoogle Scholar
  21. Steitz JA, (1969) Polypeptide chain initiation: nucleotide sequences of the three ribosome binding sites in Bacteriophage R17 RNA, Nature 224: 957–964PubMedCrossRefGoogle Scholar
  22. Studier FW and Moffat BA, (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes, J. Mol. Biol. 189: 113–130PubMedCrossRefGoogle Scholar
  23. Ulmann A, Joseph E, and Danchin A, (1979) Cyclic AMP as a modulator of polarity in polycistronic transcriptional units, Proc. Natl. Acad. Sci. USA 76: 3194–3197CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • N. Jacques
    • 1
  • M. Chevrier-Miller
    • 1
  • J. Guillerez
    • 1
  • M. Dreyfus
    • 1
  1. 1.Laboratoire de Génétique MoléculaireEcole Normale SupérieureParisFrance

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