Ribonucleases: Diversity and Regulation

  • Murray P. Deutscher
  • Jiren Zhang
Conference paper
Part of the NATO ASI Series book series (volume 49)


The complexity of RNA metabolism has become much more apparent in recent years. First of all, it is now clear that there are many more types of RNA molecules present in cells than the original classes of rRNA, tRNA and mRNA. Secondly, most, if not all, of these RNA molecules are initially synthesized as precursors that must be processed to generate the mature, functional species. In addition, some of these functional RNAs undergo other turnover or modification reactions that further alter their structure. Finally, RNA molecules are ultimately degraded, and these degradative reactions proceed at different rates among classes of RNA molecules and even among members of the same class. These latter findings add an additional level of complexity to RNA metabolism because they imply that regulatory processes may be involved in the differential stability of RNA molecules.


mRNA Stability Specific Cleavage Multicopy Plasmid Differential Stability Polynucleotide Phosphorylase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Amitsur M, Morad I and Kaufmann G (1989) In vitro reconstitution of anticodon nuclease from components encoded by phage T4 and Escherichia coli CTr5X. EMBOJ. 8: 2411–2415Google Scholar
  2. Apirion D (1974) The fate of mRNA and rRNA in Escherichia coli. Brookhaven Symp. Biol. 26: 286–306Google Scholar
  3. Bardwell JCA, Regnier P, Chen SM, Nakamura Y, Grunberg-Monago M and Court DL (1989) Autoregulation of RNase III operon by mRNA processing. EMBO J. 8: 3401–3407PubMedGoogle Scholar
  4. Bechhofer DH and Zen KH (1989) Mechanism of erythromycin-induced ermC mRNA stability in Bacillus subtilis. J. Bacteriol. 171: 5803–5811PubMedGoogle Scholar
  5. Belasco JG and Higgins CF (1989) Mechanisms of mRNA decay in bacteria: A perspective. Gene 72: 15–23CrossRefGoogle Scholar
  6. Beppu T and Arima K (1969) Induction by mercuric ion of extensive degradation of cellular ribonucleic acid in Escherichia coli. J. Bacteriol. 98: 888–897PubMedGoogle Scholar
  7. Bernstein P and Ross J (1989) Poly(A), poly(A) binding protein and the regulation of mRNA stability. TIBS 14: 373–377PubMedGoogle Scholar
  8. Cannistraro VJ and Kenneil D (1989) Purification and characterization of RNase M and mRNA degradation in Escherichia coli. Eur. J. Biochem. 181: 363–370PubMedCrossRefGoogle Scholar
  9. Chen CYA, Beatty JT, Cohen SN and Belasco JG (1988) An intercistronic stem- loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability. Cell 52: 609–619PubMedCrossRefGoogle Scholar
  10. Cudny H, Zaniewski R and Deutscher MP (1981) iL SQii RNase D: catalytic properties and substrate specificity. J. Biol. Chem. 256: 5633–5637Google Scholar
  11. Deutscher MP (1984) Processing of tRNA in prokaryotes and eukaryotes. Crit. Rev. Biochem. 17: 45–71CrossRefGoogle Scholar
  12. Deutscher MP (1985) E. coli RNases: Making sense of alphabet soup. Cell 40: 731–732Google Scholar
  13. Deutscher MP, Marlor CW and Zaniewski R (1985) RNase T is responsible for the end-turnover of tRNA in fL GQli. Proc. Natl. Acad. Sei. U.S.A. 82: 6427–6430Google Scholar
  14. Deutscher MP, Marshall GT and Cudny H (1988) RNase PH: A new phosphate- dependent nuclease distinct from polynucleotide Phosphorylase. Proc. Nat. Acad. Sei. USA 85: 4710–4714Google Scholar
  15. Deutscher MP (1988) The metabolic role of RNases. TIBS 13: 136–139PubMedGoogle Scholar
  16. Donovan WP and Kushner SR (1986) Polynucleotide Phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia COJiK-12. Proc. Natl. Acad. Sei. U.S.A. 83: 120–124Google Scholar
  17. Gilson E, Clement JM, Perrin D and Hofnung M (1987) Palindromic units: a case of highly repetitive DNA sequences in bacteria. Trends in Gen. 3: 226–230CrossRefGoogle Scholar
  18. Gold L (1988) Posttranscriptional regulatory mechanisms in Escherichia coli. Ann. Rev. Biochem. 57: 199–233PubMedCrossRefGoogle Scholar
  19. Hartley RW (1989) Barnase and barstar: two small proteins to fold and fit together. TIBS 14: 450–454PubMedGoogle Scholar
  20. Hayashi MN and Hayashi M (1985) Cloned DNA sequences that determine mRNA stability of bacteriophage 174 in vivo are functional. Nucleic Acid Res. 13: 5937–5948PubMedCrossRefGoogle Scholar
  21. Ito R and Ohnishi Y (1983) The roles of RNA polymerase and RNase I in stable RNA degradation in fL CQJi carrying the srnB gene. Biochim. Biophys. Acta 739: 27–34PubMedGoogle Scholar
  22. King TC, Sirdeskmukh R and Schlessinger D (1986) Nucleolytic processing of RNA transcripts in procaryotes. Microbiol. Rev. 50: 428–451Google Scholar
  23. Lennette ET, Meyhack B and Apirion D (1972) A mutation affecting degradation of stable RNA in Escherichia coli. FEBS Lett. 21: 286–288CrossRefGoogle Scholar
  24. Lundberg U, Melefors O and von Gabain A (To be published) Purification and characterization of a novel endoribonuclease controlling mRNA stability in coli. EMBOJ.Google Scholar
  25. Malter JS (1989) Identification of a AUUUA-specific messenger RNA binding protein. Science 246: 664–666PubMedCrossRefGoogle Scholar
  26. Mullner EW, Neupert B and Kuhn LC (1989) A specific mRNA binding factor regulates the iron-dependent stability of cytoplasmic transferrin receptor mRNA. Cell 58: 373–382PubMedCrossRefGoogle Scholar
  27. Newbury SF, Smith NH and Higgins CF (1987) Differential mRNA stability controls relative gene expression within a polycistronic operon. Cell 51: 1131–1143PubMedCrossRefGoogle Scholar
  28. Nilsson G, Lundberg U and von Gabain A (1988)]n vivo and in vitro identity of site specific cleavages in the 5 non-coding region of ompA and bla mRNA in Escherichia coli. EMBOJ. 7: 2269–2275.Google Scholar
  29. Ohnishi Y and Schlessinger D (1972) Total breakdown of ribosomal and transfer RNA in a mutant of Escherichia coli. Nature New Biol. 238: 228–231Google Scholar
  30. Plunkett III G and Echols H (1989) Retroregulation of the bacteriophage lambda int gene: Limited secondary degradation of the RNase Ill-processed transcript. J. Bacteriol. 171: 588–592PubMedGoogle Scholar
  31. Portier C, Dondon L, Grunberg-Manago M and Regnier P (1987) The first step in the functional inactivation of the EfioJi polynucleotide Phosphorylase messenger is a ribonuclease III processing at the 5 end. EMBO J. 6: 2165–2170PubMedGoogle Scholar
  32. Shaw G and Kamer R (1986) A conserved AU sequence from the 3 untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46: 659–667PubMedCrossRefGoogle Scholar
  33. Schedl P, Roberts J and Primakoff P (1976) In vitro processing of E. coli tRNA precursors. Cell 8:581–594 Stevens A and Maupin MK (1987) A 5-3 exoribonuclease of human placental nuclei: purification and substrate specificity. Nucleic Acids Res. 15: 695–708Google Scholar
  34. Takata R, Mukai T and Hori K (1987) RNA processing by RNase III is involved in the synthesis of Escherichia coli polynucleotide Phosphorylase. Mol. Gen. Genet. 209: 28–32Google Scholar
  35. Tomlins RL and Ordal ZJ (1971) Precursor ribosomal RNA and ribosome accumulation in vivo during recovery of S. typhimurium from thermal injury. J. Bacteriol. 107: 134–142PubMedGoogle Scholar
  36. Uzan M, Favre R and Brody E (1988) A nuclease that cuts specifically in the ribosome binding site of some T4 mRNAs. Proc. Natl. Acad. Sei. U.S.A. 85: 8895–8899Google Scholar
  37. Zhang J and Deutscher MP (1988a) Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D. J. Bacteriol. 170: 522–527PubMedGoogle Scholar
  38. Zhang J and Deutscher MP (1988b) Transfer RNA is a substrate for RNase D in vivo. J. Biol. Chem. 263: 17909–17912PubMedGoogle Scholar
  39. Zhang J and Deutscher MP (1988c) Escherichia coli RNase D: sequencing of the rnd structural gene and purification of the overexpressed protein. Nucleic Acids Res. 16: 6265–6278PubMedCrossRefGoogle Scholar
  40. Zhang J and Deutscher MP (1989) Analysis of the upstream region of the E. QQÜ IDd gene encoding RNase D. J. Biol. Chem. 264: 18228–18233Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Murray P. Deutscher
    • 1
  • Jiren Zhang
    • 1
  1. 1.Department of BiochemistryUniversity of Connecticut Health Center FarmingtonUSA

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