Abstract
Receptor-mediated endocytosis is a process in which macromolecules (ligands) first bind to specific receptors at the plasma membrane. These ligand-receptor complexes are subsequently transported into the cell within vesicles which pinch off from the plasma membrane (for reviews see Goldstein et al., 1985; VanDeurs et al., 1989). Except for the mature red blood cell, all eucaryotic cells are involved in endocytosis. Among the many different types of molecules that enter the cell by means of receptor-mediated endocytosis are growth factors (such as epidermal growth factor and insulin like growth factor), hormones (such as insulin and luteinizing hormone), transport proteins (such as transferrin for iron transport, low-density lipoprotein for cholesterol transport, and transcobalamin II for vitamin B12 transport), proteins which are destined for degradation (such as a2 macroglycoprotein and asialoglycoproteins), and proteins which have to be transported across a polarized cell (such as polymeric immunoglobulin A). In addition to the uptake of these physiological molecules, toxins and viruses also enter cells by receptor-mediated endocytosis. Each ligand-receptor complex follows a distinct intracellular transport route, which is dependent on the final destination of the ligand. However, the initial events for endocytosis of most receptor-ligand complexes appears to be identical.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anderson, R.G.W., and I. Orci. 1988. A view of acidic intracellular compartments. J. Cell Biol. 106: 539–543.
Ashwell G, and A.G. Morell. 1974. The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol 41: 99–128.
Beardmore, J., K.E. Howell, K. Miller, and C.R. Hopkins. 1986. Isolation of an endocytic compartment from A431 cells using a density modification procedure employing a receptor-specific monoclonal antibody complexed with colloidal gold. J. Cell Sci. 87: 495–506.
Braulke, T., C. Gartung, A. Hasilik, and K. von Figura. 1987. Is movement of mannose 6-phosphate-specific receptor triggered by binding of lysosomal enzymes? J. Cell Biol. 104: 1935–1742.
Bretscher, M.S., and B.M.F. Pearse. 1984. Coated pits in action. Cell 38: 3–4.
Bridges, K., J. Harford, G. Ashwell, and R.D. Klausner. 1982. Fate of receptor and ligand during endocytosis of asialoglycoproteins by isolated hepatocytes. Proc. Natl. Acad. Sci. USA. 79: 350–354.
Carpentier J.L., P. Gordon, R.G.W. Anderson, J.L. Goldstein, M.S. Brown, S. Cohen, and L. Orci. 1982. Colocalization of 125I-epidermal growth factor and ferritin-low density lipoprotein in coated pits: A quantitative electron microscopic study in normal and mutant human fibroblasts. J. Cell Biol. 95: 73–77.
Ciechanover, A., A.L. Schwartz, and H.F. Lodish. 1983b. The asialoglycoprotein receptor internalizes and recycles independently of the transferrin and insulin receptors. Cell 32: 267–275.
Courtoy, P.J., J. Quintart and P. Baudhuin. 1984. Shift of equilibrium density induced by 3,3′-diaminobenzidine cytochemistry: A new procedure for the analysis and purification of peroxidase-containing organelles. J. Cell Biol. 98:870–876.
Courtoy, P. J., J. Quintart, J. N. Limet, C. de Roe, and P. Baudhuin. 1985. Polymeric IgA and galactose-specific pathways in rat hepatocytes: evidence for intracellular ligand sorting. in Endocytosis, 163–194. I. Pastan and M. C. Willingham, editors. Plenum press, New York.
Dautry-Versat, A., A. Ciechanover, and H.F. Lodish. 1983. pH and the recycling of transferrin receptor during receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA. 80: 2258–2262.
Fishman, J.B., and R.E. Fine. 1987. A trans Golgi-derived exocytic coated vesicle can contain both newly synthesized Cholinesterase and internalized transferrin. Cell 48: 157–164.
Geuze, H.J., J. W. Slot, G. J. Strous, H. F. Lodish, and A. L. Schwartz. 1983a. Intracellular site of asialoglycoprotein receptor-ligand uncoupling: double-label immunoelectron microscopy during receptor-mediated endocytosis. Cell 32: 277–287.
Geuze H.J., J. W. Slot, G. J. Strous, and A. L. Schwartz. 1983b. The pathway of the asialoglycoprotein-ligand during receptor-mediated endocytosis: a mophological study with colloidal gold/ligand in the human hepatoma cell line, HepG2. Eur. J. Cell Biol. 32: 38–44.
Geuze, H. J., J. W. Slot, G. J. Strous, J. Peppard, K. von Figura, A. Hasilik, and A. L. Schwartz. 1984a. Intracellular receptor sorting during endocytosis: comparative immunoelectron microscopy of multiple receptors in rat liver. Cell. 37: 195–204.
Geuze, H.J., J.W. Slot, G.J. Strous, J.P. Luzio, and A.L. Schwartz. 1984b. A Cyclohexamide-resistant pool of receptors for asialoglycoproteins and mannose 6-phosphate residues in the Golgi complex of rat hepatocytes. EMBO J. 3: 2677–2685.
Geuze, H.J., J.W. Slot, G J. A.M. Strous, A. Hasilik, and K. von Figura. 1985. Possible pathways for lysosomal enzyme delivery. J. Cell Biol. 101: 2253–2262.
Geuze, H.J., J.W. Slot, and A.L. Schwartz. 1987. Membranes of sorting organelles display lateral heterogeneity in receptor distribution. J. Cell Biol. 104: 1715–1723.
Geuze, H.J., W. Stoorvogel, G.J. Strous, J.W. Slot, J.E. Bleekemolen, and I. Mellman. 1988. Sorting of mannose 6-phosphate receptors and lysosomal membrane proteins in endocytic vesicles. J. Cell Biol. 107: 2491–2501.
Goldstein J.L., M.S. Brown, R.G.W. Anderson, D.W. Russell, and W.J. Schneider. 1985. Receptor-mediated endocytosis: concepts emerging from the LDL receptor system. Annu. Rev. Cell Biol. 1: 1–39.
Graham RC Jr., and M.J. Karnovsky. 1966. The early stages of absorbtion of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J. Hist. Chem. 14: 291–302.
Griffiths, G., B. Hoflack, K. Simons, I. Mellman and S. Kornfeld. 1988. The mannose 6-phosphate receptor and the biogenesis of lysosomes. Cell 52: 329–341.
Helmy, S., K. Porter-Jordan, E.A. Dawidowicz, P. Pilch, A.L. Schwartz, and R.E. Fine. 1986. Separation of endocytic from exocytic coated vesicles using a novel Cholinesterase mediated density shift technique. Cell 44: 497–506.
Hoflack, B., and S. Kornfeld. 1985. Purification and characterization of a cation-dependent mannose 6-phosphate receptor from murine P3 88D1 macrophages and bovine liver. J. Biol. Chem. 260: 12008–12014.
Hopkins, C.R., and I.S. Trowbridge. 1983. Internalization and processing of transferrin and the transferrin receptor in human carcinoma cells A431. J. Cell Biol. 97: 508–521.
Kiess, W., G.D. Blickenstaff, M.M. Sklar, C.L. Thomas, S.P. Nissley, and G.G. Sahagian. 1988. Biochemical evidence that the type II insulin-like growth factor receptor is identical to the cation-independent mannose 6-phosphate receptor. J. Biol. Chem. 263: 9339–9344.
Klausner R.D., G. Ashwell, J. van Renswoude, J.B. Harford, and K.R. Bridges. 1983. Binding of apotransferin to K562 cells: explanation of the transferrin cycle. Proc. Natl. Acad. Sci. USA 80: 2263–2266.
Marsh, M, and A. Helenius. 1980. Adsorbtive endocytosis of Semliki Forest Virus. J. Mol. Biol. 142: 439–454.
Mellman, I., R. Fuchs, and A. Helenius. 1986. Acidification of the endocytic and exocytic pathways. Annu. Rev. Biochem. 55: 663–700.
Pastan I. H., and M.C. Willingham. 1981. Receptor-mediated endocytosis of hormones in cultured cells. Ann. Rev. Physiol. 43: 239–250.
Pfeffer, S.R. 1987. The endosomal concentration of a mannose 6-phosphate receptor is unchanged in the absence of ligand synthesis. J. Cell Biol. 105: 229–234.
Rudnick, G. 1986. ATP-driven H+ pumping into intracellular organelles. Annu. Rev. Physiol. 48: 403–415.
Sahagian, G.G., J.J. Distler, and G.W. Jourdian. 1982. Characterization of a membrane-associated receptor from bovine liver that binds phosphomannosyl residues of bovine testicular galactosidase. Proc. Natl. Acad. Sci. USA. 78: 4289–4293.
Schwartz, A.L., S.E. Fridovich, and H.F. Lodish. 1982. Kinetics of internalization and recycling of the asialoglycoprotein receptor in a hepatoma cell line. J. Biol. Chem. 257: 4230–4237.
Simmons, C.F. and A.L. Schwartz. 1984. Cellular pathways of galactose-terminal ligand movement in a cloned human hepatoma cell line. Mol. Pharmacol. 26: 509–519.
Stoorvogel, W., H.J. Geuze, and G.J. Strous. 1987. Sorting of endocytosed transferrin and asialoglycoprotein occurs immediately after internalization in HepG2 cells. J. Cell Biol. 104: 1261–1268.
Stoorvogel, W., H.J. Geuze, J.M. Griffith, and G.J. Strous. 1988. The pathways of endocytosed transferrin and secretory protein are connected in the trans-Golgi reticulum. J. Cell Biol. 106: 1821–1829.
Stoorvogel, W., H.J. Geuze, J.M. Griffith, A.L. Schwartz, and G.J. Strous. 1989. Relations between the intracellular pathways of the receptors for transferrin, asialoglycoprotein, and mannose 6-phosphate in human hepatoma cells. J. Cell Biol. 108: 2137–2148.
Tolleshaug, H., T. Berg, and K. Holte. 1980. Kinetics of internalization and degradation of asialoglycoproteins in isolated rat hepatocytes. Eur. J. Cell Biol. 23: 104–109.
Tycko, B., and F.R. Maxfield. 1982. Rapid acidification of endocytotic vesicles containing 2-macroglobulin. Cell 28: 634–651.
Van den Bosch, R.A., H.J. Geuze, and G.J. Strous. 1986. Presence of the asialoglycoprotein receptor in the Golgi complex in the absence of protein synthesis. Exptl. Cell Res. 162: 231–242.
VanDeurs B., O.W. Petersen, S. Olsnes, and K. Sandvig. 1989. The ways of endocytosis. Int. Rev. Cytol. 117, 131–177.
Von Figura, K., V. Gieselmann, and A. Hasilik. 1984. Antibody to mannose 6- phosphate specific receptor induces receptor deficiency in human fibroblasts. EMBO J. 3: 1281–1286.
Wileman, T., C. Harding, and P. Stahl. 1985. Receptor-mediated endocytosis. Biochem. J. 232: 1–14.
Willingham M.C., and I. Pastan. 1980. The receptosome: An internal intermediate organelle of receptor-mediated endocytosis in cultured fibroblasts. Cell 21: 67–77.
Willingham, M.C., I.H. Pastan, G.G. Sahagian, G.W. Jourdian, and E.F. Neufeld. 1981. Morphologic study of the internalization of a lysosomal enzyme by the mannose 6-phosphate receptor in cultured Chines hamster ovary cells. Proc. Natl. Acad. Sci. USA. 78: 6967–6971.
Yamashiro, D.J., S.R. Fluss, and F.R. Maxfield. 1983. Acidification of endocytic vesicles by an ATP-dependent proton pump. J. Cell Biol. 97: 929–934.
Yamashiro, D.J., and F.R. Maxfield. 1984. Acidification of endocytic compartments and the intracellular pathways of ligands and receptors. J. Cell. Biochem. 26: 231–246.
Zijderhand-Bleekemolen, J.E., A.L. Schwartz, J.W. Slot, G.J. Strous, and H.J. Geuze. 1987. Ligand and weak base-induced redistribution of asialoglycoprotein receptors in hepatoma cells. J. Cell Biol. 104: 1647–1654.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Stoorvogel, W. (1991). Protein Sorting in Endosomes. In: Ross, E.M., Wirtz, K.W.A. (eds) Biological Signal Transduction. NATO ASI Series, vol 52. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75136-3_35
Download citation
DOI: https://doi.org/10.1007/978-3-642-75136-3_35
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-75138-7
Online ISBN: 978-3-642-75136-3
eBook Packages: Springer Book Archive