Multiple sources of sn-1,2-diacylglycerol in mitogen-stimulated Swiss 3T3 cells; evidence for activation of phosphoinositidase C and PtdChophospholipase D

  • Simon J. Cook
  • Susan Palmer
  • Robin Plevin
  • Michael J. O. Wakelam
Conference paper
Part of the NATO ASI Series book series (volume 52)

Abstract

A number of growth factors, exemplified by bombesin, bind to receptors and stimulate the hydrolysis of PtdIns(4,5)P2 to generate Ins(1,4,5)P3 and sn-1,2-diacylglycerol (DG) (reviewed by Berridge, 1987). These two molecules are potent second messengers: Ins(1,4,5)P3 binds to a specific receptor, probably on the E.R., stimulating the release of sequestered calcium into the cytosol (reviewed by Berridge & Irvine, 1989) whilst DG remains within the inner leaflet of the plasma membrane where it binds to and activates protein kinase C (reviewed by Parker et al., 1989). This pathway is implicated in the regulation of a number of the early events associated with entry into the cell cycle including increases in pHi (Hesketh et al., 1986), induction of the expression of c-fos and c-myc proto-oncogenes (Rozengurt & Sinnet-Smith, 1987) and activation of the ribosomal protein S6 kinase (Susa et al., 1989). However PtdIns(4,5)P2 hydrolysis is not a pre-requisite for stimulation of cell proliferation by all growth factors, e.g. EGF in Swiss 3T3 cells (Whitman & Cantley, 1988). In addition, it should be noted that the increase in Ins(1,4,5)P3 is essentially transient, as is the associated increase in [Ca2+]i, declining towards control within 1–2 minutes whereas DNA synthesis requires the continued presence of the mitogen for up to 8–10 hours.

Keywords

Hydrolysis Superoxide Prostaglandin Cytosol Choline 

Abbreviations

Cho

choline

ChoP

phosphocholine

DG

sn-1,2-diacylglycerol

GroPCho

glycerophosphocholine

PKC

protein kinase C

PLC

phospholipase C

PLD

phospholipase D

PMA

phorbol myristate acetate

PPH

PtdOH phosphohydrolase

PtdBut

phosphatidylbutanol

PtdOH

phosphatidic acid

PtdCho

phosphatidylcholine

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Simon J. Cook
    • 1
  • Susan Palmer
    • 1
  • Robin Plevin
    • 1
  • Michael J. O. Wakelam
    • 1
  1. 1.Molecular Pharmacology Group, Institute of BiochemistryUniversity of GlasgowGlasgowScotland, UK

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