Abstract
Histamine stimulation of phosphoinositide hydrolysis was measured in primary cultures of neurons obtained from cerebrum of fetal rats of 16 days gestation. In cells prelabeled with (3H)inositol, histamine in the presence of lithium induces accumulation of (3H)inositol monophosphate linearly for 30–40min. The effect is concentration-dependent (Emax=149±9% over basal, EC50=5,8±0,9 μM, Hill coefficient=0,99±0,07, n=3) and shows pharmacological characteristics typical of an H1-mediated response. The histamine effect increases with neuronal differentiation during the first week in culture and stabilizes thereafter. In contrast to astrocytes where the histamine response is abolished when Ca2+ is omitted from the incubation medium, in neurons it is necessary to quelate all the remnant ion to prevent the effect. Furthermore, the response to a depolarizing concentration of K+ is additive to that of histamine and the stimulation by the amine is not affected by omission of Na+, or by incubation with the voltage-dependent Na+ channel blocker tetrodotoxin. All these observations indicate that the H1-receptor-stimulated inositol phosphate formation in neurons is direct and not a consequence of a histamine-induced depolar ization, increased Ca2+ permeability or release of other neurotransmitters.
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References
Agulló L, Picatoste F, García A (1990) Histamine stimulation of cyclic AMP accumulation in astrocyte-enriched and neuro nal primary cultures from rat brain J Neurochem 55:1592–1598
Alexander SPH, Hill SJ, Kendall DA (1990) Differential effects of elevated calcium ion concentrations on inositol phospholipid responses in mouse and rat cerebral cortical slices Biochem Pharmacol 40:1793–1799
Arbonés L, Picatoste F, García A (1988) Histamine H1-receptors mediate phosphoinositide hydrolysis in astrocyte-enriched primary cultures Brain Res 450:144–152
Arbonés L, Picatoste F, García A (1990) Histamine stimulates glycogen breakdown and increases 45Ca2+ permeability in rat astrocytes in primary culture Mol Pharmacol 37:921–927
Auvinen S, Panula P (1988) Development of histamine-immunore-active neurons in the rat brain J Comp Neurobiol 276:289–303
Carswell H, Galione AG, Young JM (1987) Differential effect of temperature on histamine- and carbachol-stimulated inositol phospholipid breakdown in slices of guinea-pig cerebral cor tex Br J Pharmacol 90:175–182
Claro E, Arbonés L, García A, Picatoste F (1986) Phosphoinositide hydrolysis mediated by histamine H1-receptors in rat brain cortex Eur J Pharmacol 123:187–196
Claro E, García A, Picatoste F (1987) Histamine-stimulated phosphoinositide hydrolysis in developing rat brain Mol Pharmacol 32:384–390
Claro E, García A, Picatoste F (1989) Carbachol and histamine stimulation of guanine-nucleotide-dependent phosphoinositide hydrolysis in rat brain cortical membranes Biochem J 261: 29–35
Dillon-Carter O, Chuang D (1989) Homologous desensitization of muscarinic cholinergic, histaminergic, adrenergic, and sero tonergic receptors coupled to phospholipase C in cerebellar granule cells J Neurochem 52:598–603
Eberhard DA, Holz RW (1988) Intracellular Ca2+ avtivates phospholipase C Trends Neurosci 11:517–520
García-Ladona J, Claro E, García A, Picatoste F (1987) Denerva tion supersensitivity of histamine H1-receptors in rat brain cortex Neurosci 284P
Gonzales RA, Crews FT (1988) Differential regulation of phosphoinositide phosphodiesterase activity in brain membranes by guanine nucleotides and calcium J Neurochem 50:1522–1528
Heacock AM, Fisher SK, Agranoff BW (1987) Enhanced coupling of neonatal muscarinic receptors in rat brain to phosphoinositide turnover J Neurochem 48:1904–1911
Hill SJ (1990) Distribution, properties and functional characteristics of three classes of histamine receptors Pharmacol Rev 42:45–83
Hill SJ, Kendall DA (1989) Cross-talk between different receptor-effector systems in the mammalian CNS Cell Signal 1:135–141
Hill SJ, Young JM (1980) Histamine H1-receptors in the brain of the guinea-pig and the rat: differences in ligand binding properties and regional distribution Br J Pharmacol 68: 687–696
Kendall DA, Nahorski SR (1984) Inositol phospholipid hydrolysis in rat cerebral cortical slices.II Calcium requirement J Neurochem 42:1388–1394
Palacios JM, Wamsely JK, Kuhar MJ (1981) The distribution of histamine H1-receptors in the rat brain: an autoradiographic study Neuroscience 6:15–37
Pearce B, Morrow C, Murphy S (1986) Receptor-mediated inositol phospholipid hydrolysis in astrocytes Eur J Pharmacol 121: 231–243
Tran VT, Chang RSL, Snyder SH (1978) Histamine H1-receptors identified in mammalian brain membranes with (3H)mepyramine Proc Natl Acad Sci USA 75:6290–6294
Tran VT, Freeman AD, Chang RSL, Snyder SH (1980) Ontogenic development of histamine H1-receptor binding in rat brain J Neurochem 34:1609–1613
Weiss S, Schmidt BH, Sebben M, Kemp DE, Bockaert J, Sladeczek F (1988) Neurotransmitter-induced inositol phosphate formation in neurons in primary culture J Neurochem 50:1425–1433
Whitworth P, Kendall DA (1988) Lithium selectively inhibits muscarinic receptor-stimulated inositol tetrakisphosphate accumulation in mouse cerebral cortex slices J Neurochem 51:258–265
Xu J, Chuang DM (1987) Serotonergic, adrenergic and histaminerg ic receptors coupled to phospholipase C in cultured cerebellar granule cells of rats Biochem Pharmacol 36:2353–2358
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© 1991 Springer-Verlag Berlin Heidelberg
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Arbonés, L., García, A. (1991). Histamine H1-Receptor-Mediated Breakdown of Inositol Phospholipids in Primary Neuronal Cultures. In: Ross, E.M., Wirtz, K.W.A. (eds) Biological Signal Transduction. NATO ASI Series, vol 52. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75136-3_28
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DOI: https://doi.org/10.1007/978-3-642-75136-3_28
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