Analysis of Autoantigenic Determinants of Thyroid Peroxidase Antigen, Generated Using Polymerase Chain Reaction

  • J. Paul Banga
  • Philip S. Barnett
  • David L. Ewins
  • Martin J. Page
  • Alan M. McGregor
Conference paper
Part of the NATO ASI Series book series (volume 38)

Abstract

Immune reactivity manifests itself in the form of B cell, that is antibody and T cell reactivity. In autoimmune disease, immune reactivity is directed towards particular target antigens (Weetman & McGregor, 1984). It has been difficult to experimentally study a large proportion of autoimmune disease due to the lack of knowledge on the nature of the autoantigen or, in cases where the autoantigen is known, the lack of available purified antigen. This lack of antigen may be due to the difficulty in purifying the material or difficulty in obtaining sufficient human tissue for biochemical purifications.

Keywords

Urea Glutathione Agarose Retina Polypeptide 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • J. Paul Banga
    • 1
  • Philip S. Barnett
    • 1
  • David L. Ewins
    • 1
  • Martin J. Page
    • 2
  • Alan M. McGregor
    • 1
  1. 1.Department of MedicineKing’s College School of Medicine & DentistryLondonUK
  2. 2.Department of Molecular BiologyWellcome Research LaboratoriesBeckenham, KentUK

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