Analysis of Autoantigenic Determinants of Thyroid Peroxidase Antigen, Generated Using Polymerase Chain Reaction
Immune reactivity manifests itself in the form of B cell, that is antibody and T cell reactivity. In autoimmune disease, immune reactivity is directed towards particular target antigens (Weetman & McGregor, 1984). It has been difficult to experimentally study a large proportion of autoimmune disease due to the lack of knowledge on the nature of the autoantigen or, in cases where the autoantigen is known, the lack of available purified antigen. This lack of antigen may be due to the difficulty in purifying the material or difficulty in obtaining sufficient human tissue for biochemical purifications.
KeywordsUrea Glutathione Agarose Retina Polypeptide
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- Banga, J.P., Tomlinson, R.W.S., Doble, N., Odell, E. McGregor, A.M. (1989). Thyroid microsomal/thyroid peroxidase autoantibodies show discrete patterns of cross-reactivity to myeloperoxidase, lactoperoxidase and horseradish peroxidase. Immunol. 67; 197–204Google Scholar
- Collison, K.S., Banga, J.P., Barnett, P.S., Kung, A.W.C. McGregor, A.M. (1989). Activation of the thyroid peroxidase gene in human thyroid cells: Effect of thyrotropin, forskolin and phorbol ester. J. Mol. Endocrinol. 3 ; 1–5Google Scholar
- Davis, L.G., Dibner, M.D. Battey, J.F. (1986). Basic Methods in Molecular Biology. Elsevier; New York, Amsterdam, LondonGoogle Scholar
- Doble, N., Banga, J.P., Pope, R., Lalor, E., Kilduff, P. McGregor, A.M. (1988). Autoantibodies to the thyroid microsomal/thyroid peroxidase antigen are polyclonal and directed to several distinct antigenic sites. Immunol. 64; 23–29Google Scholar
- Maniatis, T., Fritsch, E.F. Sambrook, J. (1982). Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar